本文選題：交變電場(chǎng)　切入點(diǎn)：癌細(xì)胞　出處：《內(nèi)蒙古大學(xué)》2012年博士論文　論文類型：學(xué)位論文

【摘要】：近年來,電場(chǎng)作用下細(xì)胞(特別是癌細(xì)胞)的生物學(xué)效應(yīng)和外加電場(chǎng)的參數(shù)選擇正在成為人們研究的熱點(diǎn)。盡管這些研究非常的細(xì)致,但是忽略了癌細(xì)胞和正常細(xì)胞之間存在著巨大的熱力學(xué)差別。細(xì)胞作為一個(gè)熱力學(xué)系統(tǒng),熵產(chǎn)生是必不可免的。癌細(xì)胞和正常細(xì)胞結(jié)構(gòu)上存在著巨大的差異,通過理論計(jì)算可知在沒有外場(chǎng)的作用下,癌細(xì)胞內(nèi)部的熵產(chǎn)生明顯高于正常細(xì)胞,兩者之間的差值大約在2.7×10-16J/(K·s)～2.2×10-14J/(K·s)的范圍內(nèi)。熵流的方向是從癌細(xì)胞到正常細(xì)胞。但是在一定外場(chǎng)的作用下,正常細(xì)胞內(nèi)部的熵產(chǎn)生高于癌細(xì)胞,可能阻止甚至改變熵流的方向。熵流伴隨著信息流。這就可能提供了-種治療癌癥的方法。對(duì)交變電場(chǎng)作用下細(xì)胞內(nèi)部的熵產(chǎn)生進(jìn)行理論研究發(fā)現(xiàn)細(xì)胞內(nèi)部的熵產(chǎn)生隨電場(chǎng)強(qiáng)度的增加單調(diào)上升,電場(chǎng)強(qiáng)度在5-40V/cm范圍內(nèi)可能達(dá)到治療要求。由此可見,在實(shí)驗(yàn)上研究細(xì)胞內(nèi)部的熵產(chǎn)生是非常重要的。但是由于細(xì)胞體積小并且在培養(yǎng)基中,實(shí)驗(yàn)上測(cè)量細(xì)胞內(nèi)部的熵產(chǎn)生是非常困難的。 本文利用細(xì)胞在電場(chǎng)和環(huán)境中溫度變化以及此過程中細(xì)胞的熱量變化,提出一種新的測(cè)量細(xì)胞熵產(chǎn)生的方法,設(shè)計(jì)了測(cè)量交變電場(chǎng)作用下細(xì)胞內(nèi)部熵產(chǎn)生的實(shí)驗(yàn)裝置。為了消除不確定因素,實(shí)驗(yàn)時(shí)間選為300s,并且使用的溫度傳感器可以滿足測(cè)量要求,重復(fù)實(shí)驗(yàn)每次都使用新的細(xì)胞,時(shí)間上盡量選為連續(xù)的兩天。 測(cè)量和比較了交變電場(chǎng)作用下兩對(duì)來自人同一組織的癌細(xì)胞(MDA-MB-231人乳腺癌細(xì)胞和SMMC-7721人肝癌細(xì)胞)和正常細(xì)胞(MCF10A人乳腺上皮正常細(xì)胞和HL-7702人肝正常細(xì)胞)的熵產(chǎn)生。結(jié)果表明：這種方法能夠非常有效的測(cè)量交變電場(chǎng)作用下細(xì)胞內(nèi)部的熵產(chǎn)生。電場(chǎng)強(qiáng)度在5-40V/cm的范圍內(nèi)交變電場(chǎng)引起的癌細(xì)胞內(nèi)部熵產(chǎn)生隨著場(chǎng)強(qiáng)的增加單調(diào)上升,而正常細(xì)胞內(nèi)部的熵產(chǎn)生是非單調(diào)上升的,電場(chǎng)強(qiáng)度在在5-30V/cm的范圍內(nèi)出現(xiàn)了一個(gè)峰值。電場(chǎng)強(qiáng)度在在5-25V/cm的范圍內(nèi),電場(chǎng)引起的癌細(xì)胞內(nèi)部熵產(chǎn)生和正常細(xì)胞的比值(MDA-MB-231/MCF10A和SMMC-7721/HL-7702)明顯小于1。這對(duì)理論估算提供了一定的實(shí)驗(yàn)支持,并為進(jìn)一步研究?jī)深惣?xì)胞的熱力學(xué)差別提供依據(jù)。 對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行了誤差分析,單因素方差分析。結(jié)果表明：誤差遠(yuǎn)小于實(shí)驗(yàn)數(shù)據(jù),無電場(chǎng)作用和電場(chǎng)作用下細(xì)胞具有明顯的統(tǒng)計(jì)意義。實(shí)驗(yàn)過程中環(huán)境溫度變化大約為0.8%,在熵產(chǎn)生計(jì)算中完全可以忽略。 我們提供了一個(gè)實(shí)驗(yàn)上研究細(xì)胞熱力學(xué)性質(zhì)非常有效的手段,并且可以進(jìn)一步研究交變電場(chǎng)作用下正常細(xì)胞和癌細(xì)胞的差異。從實(shí)驗(yàn)結(jié)果來看,電場(chǎng)強(qiáng)度在5-25V/cm的范圍內(nèi),交變電場(chǎng)作用下,正常細(xì)胞內(nèi)部的熵產(chǎn)生要高于癌細(xì)胞內(nèi)部的熵產(chǎn)生,并且癌細(xì)胞和正常細(xì)胞內(nèi)部交變電場(chǎng)引起的熵產(chǎn)生差值達(dá)到了10-14J/(k·s),這已經(jīng)達(dá)到了無外場(chǎng)時(shí)癌細(xì)胞和正常細(xì)胞內(nèi)部熵產(chǎn)生的差值,這有可能阻止甚至反轉(zhuǎn)癌細(xì)胞和正常細(xì)胞之間熵流和信息流方向的要求,從而達(dá)到治療的要求,這將為治療癌癥提供了一個(gè)新的線索和依據(jù)。 本工作的創(chuàng)新之處包括：提出了一個(gè)實(shí)驗(yàn)上測(cè)量活細(xì)胞內(nèi)部熵產(chǎn)生的方法；測(cè)量和比較了交變電場(chǎng)作用下兩對(duì)來自同一組織的癌細(xì)胞和正常細(xì)胞內(nèi)部的熵產(chǎn)生；這將提供了一個(gè)實(shí)驗(yàn)上研究細(xì)胞熱力學(xué)性質(zhì)非常有效的手段。
[Abstract]:In recent years, the cells under the action of the electric field (especially cancer cells) selection is becoming a hot research field and the external parameters of the biological effects. Although the research is very detailed, but ignored the existence of a huge difference between the cancer cells and normal cells. Cells as a thermodynamic system, entropy generation is necessary free. Cancer cells and normal cells structure there is a huge difference, theoretical calculation shows that in the absence of external field, was significantly higher than that of normal cells to produce cancer cells within the entropy, the difference between the two is about 2.7 * 10-16J/ (K - s) ~ 2.2 * 10-14J/ (K - s) range. Entropy flow direction is to the normal cells from cancer cells. But in external field, higher than that of cancer cells to produce normal cell internal entropy, may prevent and even change the direction of entropy flow. The entropy flow along with the flow of information on this. Possible methods for the treatment of cancer. Provide a theoretical study found that with the electric field intensity increased monotonically increasing cell internal entropy generation of internal cells under alternating electric field entropy, electric field intensity may reach the treatment requirements in the range of 5-40V/cm. Thus, the research on cell internal inspection of the entropy generation is very important but because the cells in the medium and small size, measuring cell internal entropy experiment is very difficult.
The temperature in the electric field and the environment changes the cell and the heat change of the cells in this process, we proposed a new method to measure the cell entropy, the entropy measurement experimental device inside the cell under alternating electric field generated by the design. In order to eliminate the uncertain factors, experimental time is 300s, and can use the temperature sensor meet the measurement requirements, repeat the experiment every time a new cell, the time to choose for two consecutive days.
The measurement and comparison of the alternating electric field of two people from the same tissue of cancer cells (human breast cancer cell line MDA-MB-231 and SMMC-7721 in human hepatocellular carcinoma cells and normal cells (MCF10A) in normal human mammary epithelial cells and HL-7702 normal liver cells) of the entropy production. The results show that this method can measure the alternating electric field inside the cell entropy a very effective role in cancer cells. Under the internal entropy alternating electric field strength in the range of 5-40V/cm produced by electric field with the field strength increased monotonically increased, while normal cells within the entropy production is non monotonic, the electric field strength in the range of 5-30V/cm appeared a peak electric field intensity on the 5-25V/cm. Within the range of the ratio of cancer cells within the electric field produced by entropy and normal cells (MDA-MB-231/MCF10A and SMMC-7721/HL-7702) was less than 1. of the theoretical estimation provides a real The support is supported and provides a basis for further study of the thermodynamic differences between the two types of cells.
The data were analyzed by error analysis, single factor analysis of variance. The results show that the error is much smaller than the experimental data, with statistical significant cells without the electric field and the electric field. The environment temperature changes during the process of experiment is about 0.8%, completely can be ignored in the calculation of entropy.
We provide a very effective means to study the properties of cells in a thermodynamic experiment, and the differences can be further studied under alternating electric field of normal and cancer cells. From the experimental results, the electric field strength in the range of 5-25V/cm, the alternating electric field, is higher than that inside the cancer cells produce normal cell internal entropy entropy that difference reached 10-14J/ and cancer cells and normal cells within the alternating electric field induced entropy (k - s), it has already reached the difference between cancer cells and normal cells within the field entropy, which can prevent and even reverse between cancer cells and normal cells of entropy and information flow direction requirements. To achieve the treatment requirements, which will provide a new clue and basis for the treatment of cancer.
The innovation of our work includes: we propose a method for the measurement of living cells internal entropy generation; measurement and comparison of two internal entropy from the same tissue of cancer cells and normal cells under alternating electric field; this will provide an experimental study on the thermal and mechanical properties of cells are effective.

【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2012
【分類號(hào)】：R730.55;O441.4

【共引文獻(xiàn)】

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