本文選題：姜黃素　切入點(diǎn)：力竭運(yùn)動(dòng)　出處：《湖南師范大學(xué)》2012年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的：探討姜黃素對(duì)力竭運(yùn)動(dòng)大鼠腎臟損傷的保護(hù)作用及其機(jī)制。 方法：SPF級(jí)健康雄性SD大鼠56只,體重210±10g,按隨機(jī)數(shù)字法分為正常對(duì)照組(C)、力竭模型組(E)、力竭+氯沙坦組(陽(yáng)性對(duì)照組)(LE)、力竭+低劑量姜黃素組(CMN1)、力竭+中劑量姜黃素組(CMN2)、力竭+高劑量組姜黃素(CMN3)、姜黃素對(duì)照組(CMN),每組8只。力竭+姜黃素組每天灌胃不同劑量姜黃素溶液(低劑量100mg/kg、中劑量200mg/kg、高劑量300mg/kg);陽(yáng)性對(duì)照組每天氯沙坦灌胃20mg/kg;姜黃素對(duì)照組灌胃劑量為200mg/kg/d;正常對(duì)照組常規(guī)條件下飼養(yǎng),正常飲食,不做任何運(yùn)動(dòng)；各運(yùn)動(dòng)組進(jìn)行力竭運(yùn)動(dòng)訓(xùn)練,每周記錄各組大鼠體重,4周后,各組大鼠取血清、血漿、腎臟組織低溫保存,HE染色光鏡下觀察腎臟形態(tài)結(jié)構(gòu)變化,分光光度法檢測(cè)血清和腎臟組織BUN、Cr、SOD及MDA的含量,采用雙抗體一步夾心法酶聯(lián)免疫吸附(ELISA)檢測(cè)血清及腎組織中TNF-α、 IL-1β、IL-18、TGF-β的含量,比色法檢測(cè)caspase-3的活性。 結(jié)果： (1)姜黃素可明顯延長(zhǎng)力竭運(yùn)動(dòng)大鼠力竭運(yùn)動(dòng)持續(xù)時(shí)間,與力竭模型組相比差異具有統(tǒng)計(jì)學(xué)意義(p0.05) (2)與力竭模型組相比,應(yīng)用姜黃素后,血清及腎組織中MDA、BUN、Cr含量明顯降低(p0.05), SOD(?)舌性顯著升高(p0.05)；三種不同劑量姜黃素組比較,中劑量組(200mg/kg)對(duì)BUN、Cr、SOD及MDA影響效果優(yōu)于低、高劑量組(p0.05)；陽(yáng)性對(duì)照組與中劑量姜黃素組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)。 (3)姜黃素干預(yù)后,大鼠腎組織及血液中TNF-α、IL-1β、IL-18、TGF-β及caspase3水平較力竭模型組顯著降低(p0.05)；中劑量組(200mg/kg)與低、高劑量組相比均有差異,對(duì)TNF-α、IL-1β、IL-18、 TGF-β及caspase3影響效果顯著優(yōu)于二組(p0.05)；陽(yáng)性對(duì)照組與中劑量組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)。 (4)光學(xué)顯微鏡下觀察腎組織切片,姜黃素干預(yù)的力竭運(yùn)動(dòng)大鼠腎臟腎損傷程度均比力竭模型組減輕。 結(jié)論： (1)姜黃素可顯著延長(zhǎng)力竭持續(xù)時(shí)間。 (2)姜黃素可能通過(guò)提高抗氧化酶活性,清除自由基等作用緩解大鼠力竭運(yùn)動(dòng)造成的腎臟損傷。 (3)姜黃素可能通過(guò)抑制促炎癥相關(guān)因子TNF-α、IL-1β、IL-18的生成,拮抗促纖維生長(zhǎng)因子TGF-β的表達(dá),抑制caspase-3活性,對(duì)力竭運(yùn)動(dòng)大鼠腎損傷起保護(hù)作用。 (4)中劑量的姜黃素(200mg/kg)較其他兩種劑量有更明顯的腎臟保護(hù)作用。
[Abstract]:Objective: to investigate the protective effect and mechanism of curcumin on kidney injury in exhaustive exercise rats. Methods 56 healthy male Sprague-Dawley rats of SPF grade, The body weight was 210 鹵10 g. The rats were randomly divided into three groups: normal control group (Con), exhausted model group (EJ), exhausted losartan group (positive control group), exhausted curcumin group (low dose curcumin group), exhausted middle dose curcumin group (CMN2), exhausted high dose group (curcumin CMN3). Different doses of curcumin solution (100mg / kg, middle dose 200mg / kg, high dose 300mg / kg) were given daily in the exhausted curcumin group, 20 mg / kg daily in the positive control group, 20 mg / kg in the curcumin control group, and 200 mg / kg / d in the curcumin control group. The normal control group was fed under normal conditions. Every exercise group had exhaustive exercise training. After 4 weeks of exercise, the rats in each group were collected serum, plasma and kidney tissue to observe the changes of renal morphology under light microscope with HE staining. The contents of BUNC and MDA in serum and renal tissue were detected by spectrophotometry. The levels of TNF- 偽-尾 IL-1 尾 IL-18 TGF- 尾 and the activity of caspase-3 in serum and renal tissue were detected by double antibody one step sandwich enzyme-linked immunosorbent assay (Elisa). Results:. 1) curcumin significantly prolonged the duration of exhaustive exercise in rats, and the difference was statistically significant compared with the exhaustive model group (P 0.05). (2) compared with the exhaustive model group, the content of MDA-BUNCr in serum and renal tissue decreased significantly after curcumin administration. Compared with three different doses of curcumin, the effect of middle dose group (200 mg 路kg ~ (-1)) on MDA and SOD was better than that of high dose group (P 0.05), but there was no significant difference between positive control group and middle dose group (P 0.05). (3) after curcumin intervention, the levels of TNF- 偽 IL-1 尾 IL-18TGF- 尾 and caspase3 in renal tissue and blood of rats were significantly lower than those of exhausted model group, and the levels of TNF- 偽 IL-1 尾 IL-18TGF- 尾 and caspase3 in middle dose group were significantly lower than those in low dose group and high dose group. The effect on TNF- 偽 IL-1 尾 IL-18, TGF- 尾 and caspase3 was significantly better than that in the two groups (P 0.05), but there was no significant difference between the positive control group and the middle dose group (P 0.05). (4) the renal tissue sections were observed under optical microscope. The degree of kidney injury in rats treated with curcumin was less than that in exhausted model group. Conclusion:. Curcumin significantly prolonged the exhaustion duration. 2) curcumin may alleviate the kidney injury induced by exhaustive exercise by increasing the activity of antioxidant enzymes and scavenging free radicals. 3) curcumin may inhibit the expression of TGF- 尾, inhibit the expression of TGF- 尾 and inhibit the activity of caspase-3 by inhibiting the production of TNF- 偽 and IL-1 尾 and IL-18. Curcumin may play a protective role on renal injury in rats after exhaustive exercise. The middle dose of curcumin 200 mg / kg had a more obvious renal protective effect than the other two doses.
【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R87

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