發(fā)布時(shí)間：2018-03-04 12:23

  本文選題：基因敲除　切入點(diǎn)：延遲性肌肉酸痛　出處：《中國(guó)康復(fù)醫(yī)學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的:探討MG53敲除對(duì)骨骼肌損傷的影響,研究MG53在延遲性肌肉酸痛期是否具有減輕骨骼肌膜損傷的作用。方法:8周齡雄性C57小鼠84只,根據(jù)基因型分為兩大組,野生型(WT)組和敲除型(KO)組,每一大組按照取材時(shí)間點(diǎn)又分為7個(gè)小組,共14組,即WT組(WC、WE0、WE2、WE12、WE24、WE48、WE72)和KO組(KC、KE0、KE2、KE12、KE24、KE48、KE72),每組6只。在離心運(yùn)動(dòng)后相應(yīng)時(shí)間點(diǎn)內(nèi)眥取血,脫頸處死小鼠,取左腿股直肌用于Western blot實(shí)驗(yàn),右側(cè)用于形態(tài)學(xué)觀察。全自動(dòng)生化分析儀檢測(cè)血清肌酸激酶(CK),透射電鏡觀察14個(gè)組大鼠骨骼肌超微結(jié)構(gòu)的變化,Western blot檢測(cè)MG53蛋白水平。結(jié)果:KE0和KE2組CK較安靜組非常顯著性升高(P0.01),KE24和WE0組CK較安靜組顯著升高(P0.05),同一時(shí)間點(diǎn)CK值相比,KE0較WE0顯著升高,KE24較WE24顯著升高(P0.05);骨骼肌超微結(jié)構(gòu)隨時(shí)程變化較為明顯,KO組在運(yùn)動(dòng)后損傷較WT組更為嚴(yán)重,呈現(xiàn)出損傷早、恢復(fù)慢的特點(diǎn),KE24損傷最為嚴(yán)重,且KE72仍然沒(méi)有恢復(fù)到安靜水平;WT組MG53蛋白在運(yùn)動(dòng)后即刻達(dá)到峰值并顯著高于安靜組(P0.05),在24h出現(xiàn)第二次升高(P0.05)。結(jié)論:離心運(yùn)動(dòng)后KO組小鼠骨骼肌損傷較WT組更為嚴(yán)重,表明MG53能夠明顯減輕骨骼肌在延遲性肌肉酸痛期的損傷,WT組MG53蛋白在離心運(yùn)動(dòng)后即刻和24h顯著升高,提示其在延遲性肌肉酸痛期骨骼肌損傷的修復(fù)中發(fā)揮了重要作用。
[Abstract]:Objective: to investigate the effects of MG53 knockout on skeletal muscle injury and whether MG53 can alleviate skeletal muscle membrane damage during delayed muscle soreness. Methods: 84 male C57 mice aged 8 weeks were divided into two groups according to their genotypes. Each group was divided into 7 groups according to the time point of sampling, which was divided into 14 groups according to the time of sampling, that is, WT group (WT group) and KO group (KCU KE0KE12KE24WE4W4W72) and KO group (KE2KE12KE24KE24KE42), each group (6 rats) were killed at the corresponding time point after centrifugation exercise, and the mice were killed at the corresponding time points after centrifugation. The left leg rectus femoris muscle was used in Western blot experiment. The right side was used for morphologic observation. The serum creatine kinase (CK) was detected by automatic biochemical analyzer. The ultrastructure of skeletal muscle in 14 groups was observed by transmission electron microscope. The level of MG53 protein was detected by blot in 14 groups. Results the CK of the two groups were higher than that of the quiet group. P0.01KE24 and WE0 group were significantly higher than that of quiet group (P 0.05), CK value of KE0 was significantly higher than that of WE0 at the same time, KE24 was significantly higher than WE24 (P 0.05), and the ultrastructure of skeletal muscle was more serious in KO group than in WT group, and the ultrastructure of skeletal muscle was more serious than that of WT group at the same time point, and the changes of ultrastructure of skeletal muscle in KO group were more serious than that in WT group. The damage of KE24 was the most serious, showing the characteristics of early injury and slow recovery. And KE72 still did not return to quiet level. The MG53 protein of WT group reached the peak immediately after exercise and was significantly higher than that of rest group (P0.05), and the second increase of P0.05A was found in 24 h. Conclusion: the skeletal muscle injury in KO group is more serious than that in WT group after centrifugal exercise. The results indicated that MG53 could significantly reduce the injury of skeletal muscle during delayed muscle soreness. MG53 protein in WT group increased significantly immediately and 24 hours after eccentric exercise, suggesting that MG53 protein may play an important role in the repair of skeletal muscle injury in delayed muscle soreness period.
【作者單位】： 北京體育大學(xué)運(yùn)動(dòng)人體科學(xué)學(xué)院;國(guó)家體育總局運(yùn)動(dòng)醫(yī)學(xué)研究所;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(31371205)
【分類號(hào)】：R87

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