本文關(guān)鍵詞： 跑臺(tái)運(yùn)動(dòng) 睡眠剝奪 焦慮行為 學(xué)習(xí)記憶 氧化應(yīng)激 c-fos　出處：《西安體育學(xué)院學(xué)報(bào)》2016年05期 　論文類(lèi)型：期刊論文

【摘要】：目的觀察4周中等強(qiáng)度跑臺(tái)運(yùn)動(dòng)預(yù)干預(yù)對(duì)睡眠剝奪大鼠焦慮樣行為及空間學(xué)習(xí)記憶能力、血清皮質(zhì)酮含量和前額葉皮質(zhì)(prefrontal cortex,PFC)氧化應(yīng)激水平及c-fos表達(dá)的影響。方法將40只大鼠隨機(jī)分為4組,即正常對(duì)照組、運(yùn)動(dòng)組、睡眠剝奪組(sleep deprivation,SD)及睡眠剝奪運(yùn)動(dòng)組(SD+E)。除正常對(duì)照組及睡眠剝奪組大鼠,運(yùn)動(dòng)組大鼠進(jìn)行4周跑臺(tái)運(yùn)動(dòng)預(yù)干預(yù),運(yùn)動(dòng)結(jié)束后睡眠剝奪組及睡眠剝奪運(yùn)動(dòng)組大鼠睡眠剝奪建立大鼠72h SD模型(小平臺(tái)水環(huán)境法),然后運(yùn)用開(kāi)場(chǎng)實(shí)驗(yàn)及明暗箱探索實(shí)驗(yàn)評(píng)估所有大鼠焦慮樣行為,八臂迷宮實(shí)驗(yàn)測(cè)試大鼠空間學(xué)習(xí)記憶能力;行為學(xué)測(cè)試結(jié)束后檢測(cè)所有大鼠血清皮質(zhì)酮及前額葉皮質(zhì)自由基水平,用免疫組織化學(xué)結(jié)合圖像半定量方法計(jì)算前額葉皮質(zhì)c-fos神經(jīng)元的分布。結(jié)果 (1)與正常對(duì)照組比較,開(kāi)場(chǎng)實(shí)驗(yàn)(open field test,OFT)測(cè)試中,SD組大鼠穿越格數(shù)(crossing numbers)、直立次數(shù)(rearing numbers)及中央格停留時(shí)間百分比均顯著減少,糞便顆粒增多;明暗箱探索實(shí)驗(yàn)中(Light-dark,LD),SD組大鼠明箱停留時(shí)間及穿越次數(shù)均顯著減少;八臂迷宮實(shí)驗(yàn)(eight arm maze tests,ERM)中SD組大鼠學(xué)習(xí)記憶能力下降,血清皮質(zhì)酮水平顯著增高,前額葉皮質(zhì)SOD及GSH-px活性顯著下降,MDA含量增加,c-fos表達(dá)顯著增強(qiáng)。(2)與SD組大鼠比較,OFT測(cè)試中,SD+E組大鼠穿越格數(shù)、直立次數(shù)及中央格停留時(shí)間百分比均顯著增加,糞便顆粒減少;LD探索實(shí)驗(yàn)中,SD+E組大鼠明箱停留時(shí)間顯著延長(zhǎng),ERM實(shí)驗(yàn)中大鼠學(xué)習(xí)記憶能力顯著改善;SD+E組大鼠血清皮質(zhì)酮水平顯著下降,前額葉皮質(zhì)SOD活性顯著上調(diào),GSHpx活性無(wú)顯著性差異,MDA含量下降,c-fos表達(dá)顯著下降。結(jié)論結(jié)果顯示,跑臺(tái)運(yùn)動(dòng)預(yù)干預(yù)可以減弱睡眠剝奪對(duì)學(xué)習(xí)記憶的影響、改善睡眠剝奪大鼠焦慮樣行為,可能與跑臺(tái)運(yùn)動(dòng)改善HPA軸功能失調(diào)、增強(qiáng)前額葉皮質(zhì)抗氧化能力及下調(diào)前額葉皮質(zhì)c-fos的過(guò)度表達(dá)有關(guān)。
[Abstract]:Objective to observe the effects of 4 weeks of moderate treadmill exercise on anxiety like behavior and spatial learning and memory ability in sleep deprived rats. The effects of serum corticosterone content, prefrontal cortex prefrontal cortex-PFC-) oxidative stress level and c-fos expression in 40 rats were randomly divided into 4 groups: normal control group and exercise group. Sleep deprivation group (SD) and sleep deprivation exercise group (SD). Except normal control group and sleep deprivation group, rats in exercise group were pretreated with running platform exercise for 4 weeks. After exercise, rats in sleep deprivation group and sleep deprivation exercise group established SD model for 72 hours (small platform water environmental law), and then used open field experiment and light and dark box exploration experiment to evaluate the anxiety behavior of all rats. Eight arm labyrinth test was used to test the spatial learning and memory ability of rats, and the levels of serum corticosterone and free radical in prefrontal cortex of all rats were measured after behavioral test. The distribution of c-fos neurons in prefrontal cortex was calculated by immunohistochemistry and image semi-quantitative method. In the open field test (OFT) test, the SD rats in the SD group significantly decreased the number of crossing numbers and the percentage of residence time of the central lattice, and the fecal particles increased. The staying time and crossing times of SD group were significantly decreased, while the learning and memory ability of SD group was decreased and the serum corticosterone level was significantly increased in eight arm labyrinth experiment (eight arm labyrinth experiment). The activity of SOD and GSH-px in prefrontal cortex decreased significantly. The expression of c-fos in prefrontal cortex was significantly increased. (2) compared with SD group, the number of traverses, the number of upright times and the percentage of residence time of central lattice in SD E group were significantly increased compared with those in SD group. In the experiment of dejecta granule reduction and LD exploration, the rats in SD E group significantly prolonged the residence time of the open box in ERM experiment. The learning and memory ability of rats in SD E group was significantly improved, and the serum corticosterone level in SD E group was significantly decreased. There was no significant difference in the activity of SOD in prefrontal cortex. The expression of c-fos decreased significantly. Conclusion the results showed that the effects of sleep deprivation on learning and memory were attenuated by treadmill exercise. The improvement of anxiety like behavior in sleep deprived rats may be related to the improvement of HPA axis dysfunction in treadmill movement, the enhancement of antioxidant capacity in prefrontal cortex and the down-regulation of c-fos expression in prefrontal cortex.
【作者單位】： 中北大學(xué)體育學(xué)院;太原大學(xué)體育學(xué)院;山西醫(yī)科大學(xué)汾陽(yáng)學(xué)院;山西體育職業(yè)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(30671019)
【分類(lèi)號(hào)】：R87

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