本文關(guān)鍵詞： 自主跑輪運動 神經(jīng)發(fā)生 神經(jīng)細胞黏附因子(NCAM) 干細胞因子(SCF) 學(xué)習(xí)記憶 胰島素樣生長因子(IGF-1) 血管內(nèi)皮生長因子(VEGF)　出處：《華東師范大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的：適宜的運動訓(xùn)練能夠提高機體的學(xué)習(xí)記憶能力,但其具體機制目前還不是完全清楚。神經(jīng)發(fā)生是指神經(jīng)元的生成,其過程包括神經(jīng)元細胞增殖、遷移、分化與存活等幾個關(guān)鍵步驟,每個步驟都可能會影響到新生細胞和新生神經(jīng)元的最終數(shù)目。海馬內(nèi)神經(jīng)發(fā)生可能促進機體學(xué)習(xí)記憶能力的提高。目前,大量實驗研究表明,運動訓(xùn)練能夠促進海馬神經(jīng)發(fā)生,進而提高機體的學(xué)習(xí)記憶能力。NCAM與SCF是海馬內(nèi)與神經(jīng)發(fā)生密切相關(guān)的因子,可以作為海馬神經(jīng)發(fā)生的標(biāo)志性因子。本文采用Morris水迷宮及實時定量PCR的方法就8周自主跑輪運動對幼齡小鼠學(xué)習(xí)記憶及其與海馬神經(jīng)發(fā)生密切相關(guān)基因mRNA表達的影響進行研究,探討神經(jīng)發(fā)生在運動訓(xùn)練促進學(xué)習(xí)記憶能力方面的作用及意義。 研究方法：經(jīng)過篩選,選出12只小鼠作為研究對象,隨機分成安靜對照組(C組,n=6)和跑輪運動組(R組,n=6)。R組小鼠每天進行自主跑輪運動,連續(xù)8周。實驗進行到第8周時,對全部小鼠進行Morris水迷宮實驗測試,檢測它們的空間學(xué)習(xí)與記憶能力。測試完成24小時后對全部小鼠采用斷頸法處死,取出其海馬組織冷凍、待測。用RT-PCR檢測兩組小鼠海馬NCAM、SCF、IGF-1、 VEGF mRNA的表達水平。 實驗結(jié)果： (1)通過Morris水迷宮測試發(fā)現(xiàn),同安靜對照組小鼠相比,跑輪運動組小鼠在學(xué)習(xí)能力以及記憶測試中的表現(xiàn)更優(yōu)秀。 (2)8周自主跑輪運動后,跑輪運動小鼠海馬NCAM mRNA的表達均顯著高于安靜對照組小鼠(p0.05)。 (3)8周自主跑輪運動后,跑輪運動小鼠海馬SCF mRNA的表達均顯著高于安靜對照組小鼠(p0.05)。 (4)8周自主跑輪運動后,跑輪運動組小鼠海馬IGF-1mRNA的表達顯著高于安靜對照組小鼠(p0.01)。 (5)8周自主跑輪運動后,跑輪運動組小鼠海馬VEGF mRNA的表達顯著高于安靜對照組小鼠(p0.05)。 結(jié)論： (1)8周自主跑輪運動能夠促進幼齡小鼠空間學(xué)習(xí)和記憶能力。 (2)8周自主跑輪運動后,跑輪運動組小鼠海馬內(nèi)NCAM和SCFmRNA表達的增加,推測跑輪運動組小鼠海馬內(nèi)神經(jīng)發(fā)生多于安靜組小鼠,提示八周自主跑輪運動可能促進小鼠海馬神經(jīng)發(fā)生,進而提示,8周自主跑輪運動對小鼠學(xué)習(xí)記憶功能的促進作用可能與海馬神經(jīng)發(fā)生有關(guān)。 (3)8周自主跑輪運動能促進小鼠海馬內(nèi)IGF-lmRNA的表達水平,提示8周自主跑輪運動可能通過提高小鼠海馬內(nèi)IGF-lmRNA的表達水平進而促進小鼠海馬神經(jīng)發(fā)生。 (4)8周自主跑輪運動能促進小鼠海馬內(nèi)VEGFmRNA的表達水平,提示8周自主跑輪運動可能通過提高小鼠海馬內(nèi)VEGFmRNA的表達水平進而促進小鼠海馬神經(jīng)發(fā)生。
[Abstract]:Objective: appropriate exercise training can improve the ability of learning and memory, but its specific mechanism is not completely clear. Neurogenesis refers to the formation of neurons, including neuronal cell proliferation. Migration, differentiation, survival and other key steps, each step may affect the final number of newborn cells and neurons. Hippocampal neurogenesis may promote the improvement of the ability of learning and memory. A large number of experimental studies have shown that exercise training can promote hippocampal neurogenesis and then improve the learning and memory ability of the body. NCAM and SCF are closely related to neurogenesis in the hippocampus. Morris water maze and real time quantitative PCR were used to study the learning and memory of young mice and their close relationship with hippocampal nerve. The effect of related gene mRNA expression was studied. To explore the role and significance of neurogenesis in promoting learning and memory ability in sports training. Methods: after screening, 12 mice were selected and randomly divided into two groups: group C (control group) and group R (running wheel exercise group). All the mice in the control group were tested by Morris water maze test at the 8th week. The ability of spatial learning and memory was measured. 24 hours after the test, all mice were killed by cervical amputation method, and their hippocampal tissue was frozen and tested. RT-PCR was used to detect NCAM in hippocampus of both groups. The expression level of VEGF mRNA and IGF-1. Experimental results: 1) the results of Morris water maze test showed that the mice in wheel running group performed better in learning ability and memory than those in quiet control group. The expression of NCAM mRNA was significantly higher in the hippocampus of the mice after 8 weeks of independent wheel running than that in the quiet control group (P 0.05). The expression of SCF mRNA was significantly higher in the hippocampus of the mice after 8 weeks of independent wheel running than that in the quiet control group (P 0.05). After 8 weeks of independent wheel running, the expression of IGF-1mRNA in hippocampus of mice in the wheel running group was significantly higher than that in the quiet control group (p0.01). The expression of VEGF mRNA in the hippocampus of the mice in the wheel running group was significantly higher than that in the quiet control group after 8 weeks of independent wheel running. Conclusion: The 8 week independent wheel running can promote the spatial learning and memory ability of young mice. The expression of NCAM and SCFmRNA in the hippocampus of the mice in the wheel running group increased after 8 weeks of independent wheel running exercise. It is speculated that the hippocampal nerve in the running wheel exercise group was more than that in the quiet group. The results suggested that the 8-week self-running wheel movement might promote the development of hippocampal nerve in mice, and further suggested that the promoting effect of 8-week self-running wheel exercise on the learning and memory function of mice might be related to the development of hippocampal nerve. Eight weeks of independent wheel running could promote the expression of IGF-lmRNA in hippocampus of mice. The results suggest that 8 weeks independent running wheel exercise may promote the development of hippocampal nerve by increasing the expression of IGF-lmRNA in hippocampus of mice. Self-running wheel movement for 8 weeks could promote the expression of VEGFmRNA in hippocampus of mice. The results suggest that 8 weeks independent running wheel exercise may promote the development of hippocampal nerve by increasing the expression of VEGFmRNA in hippocampus of mice.
【學(xué)位授予單位】：華東師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：G804;R87

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