探針?biāo)幬锓ㄔu價黃草烏酒對大鼠肝臟CYP1A2、CYP3A1和CYP2E1的影響
發(fā)布時間：2018-01-08 17:37
本文關(guān)鍵詞：探針?biāo)幬锓ㄔu價黃草烏酒對大鼠肝臟CYP1A2、CYP3A1和CYP2E1的影響 出處：《河南科技大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年04期 論文類型：期刊論文
更多相關(guān)文章： 黃草烏 酒精 Cocktail探針?biāo)幬?/b> CYP UPLC-MS/MS
【摘要】：目的探討黃草烏酒對大鼠CYP450亞型(CYP1A2、CYP3A1、CYP2E1)的代謝活性的影響。方法采用Cocktail探針法(咖啡因、咪達(dá)唑侖和氯唑沙宗),利用超高效液相色譜—串聯(lián)質(zhì)譜(UPLC-MS/MS)測定黃草烏酒對大鼠CYP450亞型活性影響。將大鼠隨機(jī)分為5組,分別為空白對照組、白酒組、單草烏組、低草烏酒組和高草烏酒組,各組動物連續(xù)灌胃給藥14 d,于第15 d腹腔注射Cocktail探針?biāo)幰?并于腹腔注射探針?biāo)幰呵昂蟛煌瑫r間點(diǎn)斷尾采血。使用UPLC-MS/MS測定各探針?biāo)幬锏难帩舛?評價CYP450亞型的代謝活性。結(jié)果氯唑沙宗的半衰期(T1/2)均低于空白對照組(P0.05),表明酒精、黃草烏和黃草烏酒對CYP2E1均有明顯的誘導(dǎo)作用。與空白對照組相比,酒精對CYP1A2和CYP3A1的誘導(dǎo)不明顯。與白酒組相比,黃草烏酒對CYP3A1和CYP2E1的誘導(dǎo)以及對CYP1A2的抑制作用均無顯著性差異。結(jié)論黃草烏與酒精合用后可誘導(dǎo)CYP2E1的活性,發(fā)生藥物間相互作用,增加黃草烏對大鼠的肝毒性。
[Abstract]:Objective to investigate the effect of Huangcaowu wine on the metabolic activity of CYP1A2CY CYP2E1, a subtype of CYP450 in rats. Methods the method of Cocktail probe (caffeine) was used. Midazolam and chlorzoxazone were used to determine the effect of Huangcaowu wine on the activity of CYP450 subtype in rats by UPLC-MS / MS. The rats were randomly divided into 5 groups. The rats were divided into three groups: blank control group, liquor group, single Aconitum group, low Caowu liquor group and high Caowu liquor group. The animals in each group were intraperitoneally injected with Cocktail probe solution for 14 days. Blood samples were collected at different time points before and after intraperitoneal injection of probe solution. Blood concentration of each probe was measured by UPLC-MS/MS. Results the half-life of clozoxazone was significantly lower than that of control group (P 0.05), indicating alcohol. Compared with the blank control group, the induction of CYP1A2 and CYP3A1 by alcohol was not obvious, and that of the liquor group was higher than that of the liquor group. There was no significant difference in the induction of CYP3A1 and CYP2E1 and the inhibitory effect on CYP1A2. Conclusion the activity of CYP2E1 can be induced by the combination of Acanthopsis flavescens and alcohol. To increase the hepatotoxicity of Atractylodes flavescens to rats by drug interaction.
【作者單位】： 昆明醫(yī)科大學(xué)法醫(yī)學(xué)院;昆明醫(yī)科大學(xué)第一附屬醫(yī)院;
【基金】：云南省應(yīng)用基礎(chǔ)研究基金資助項(xiàng)目(2013FB118) 云南省教育廳科學(xué)研究基金(2014Y161)
【分類號】：D919
【正文快照】： However,the inductions of alcohol were notobvous on CYP1A2 and CYP3A1.Compared withthe white spirit group,the inductions of aconitumvilmorimianum kom liquor on CYP3A1 andCYP2E1 were no significant,as well as theinhibition on CYP2E1.Conclusion Aconitumvil，
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