目的:探討亞砷酸鈉（Na As O₂）、巰基丙酮酸硫轉(zhuǎn)移酶（MST）基因過表達(dá)對SH-SY5Y細(xì)胞的作用及機(jī)制。方法:將空載體轉(zhuǎn)染細(xì)胞、MST過表達(dá)細(xì)胞各分為對照組、Na As O₂組、TUDCA（內(nèi)質(zhì)網(wǎng)應(yīng)激阻斷劑）組、Na As O₂聯(lián)合TUDCA組,采用MTT、Western blot和酶活性檢測等方法觀察細(xì)胞活力、Bax、Bcl-2凋亡相關(guān)蛋白表達(dá)及Caspase-3活性。結(jié)果:50μmo L/L Na As O₂暴露24 h顯著降低空轉(zhuǎn)組細(xì)胞活力和Bcl-2蛋白水平（P <0.01）,增加Bax蛋白表達(dá)及Caspase-3活性（P <0.01）; TUDCA預(yù)處理可以逆轉(zhuǎn)以上變化,Bcl-2的蛋白表達(dá)則在MST基因過表達(dá)細(xì)胞砷暴露后發(fā)生顯著增加,但可被TUDCA所拮抗（P <0.01）。結(jié)論:內(nèi)質(zhì)網(wǎng)應(yīng)激可能參與砷、內(nèi)源性H2S對SH-SY5Y細(xì)胞損傷的影響。 

【文章來源】：貴州醫(yī)科大學(xué)學(xué)報. 2020,45(11)

【文章頁數(shù)】：5 頁

【部分圖文】：

空轉(zhuǎn)組細(xì)胞活力在Na As O2和TUDCA處理后的變化

空轉(zhuǎn)組Bcl-2和Bax表達(dá)在Na As O2和TUDCA處理后的變化

空轉(zhuǎn)組Caspase-3活性在Na As O2和TUDCA處理后的變化

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