【摘要】：目的:探討環(huán)境內(nèi)分泌干擾物全氟辛磺酸(perfluorooctane suifonate,PFOS)對小鼠小膠質(zhì)瘤細胞BV-2的損傷作用及其炎性反應(yīng)。方法:利用體外培養(yǎng)的小膠質(zhì)細胞,給予不同濃度和作用時間的PFOS進行染毒,通過MTT法檢測BV-2細胞活力;實時定量PCR的方法觀察誘導(dǎo)型一氧化氮合酶(inducible nitric oxide synthesis,i NOS)、白介素(interleukin,IL)-6和腫瘤壞死因子(tumor necrosis factor,TNF)-αm RNA的表達。ELISA法檢測炎性因子IL-6,免疫蛋白印跡法檢測核轉(zhuǎn)錄因子kappa B(nuclear factor-kappa B,NF-κB)等信號蛋白表達情況。結(jié)果:不同濃度PFOS對BV-2細胞染毒12、24 h后,細胞活力下降,引起明顯的細胞損傷。ELISA結(jié)果顯示,PFOS作用BV-2 24 h后,細胞炎性因子IL-6分泌增加。此外,PFOS還可引起i NOS、IL-6基因表達上調(diào),而TNF-α表達有下降趨勢。PFOS與細胞孵育后,炎性通路NF-κB明顯激活,表現(xiàn)為磷酸化程度升高。且隨染毒濃度的增加,p-NF-κB的表達有上升趨勢;此外,隨染毒時間的增加,p-NF-κB的表達亦有上升趨勢。結(jié)論:PFOS可引起B(yǎng)V-2細胞活力降低,激活NF-κB通路,促進炎性因子的釋放,該途徑可為闡明PFOS引起的神經(jīng)系統(tǒng)損傷的分子機制提供理論依據(jù)。
[Abstract]:Aim: to investigate the damage and inflammatory response of environmental endocrine disruptor perfluorooctanesulfonic acid (perfluorooctane suifonate,PFOS) to mouse microglioma cell line BV-2. Methods: microglia cultured in vitro were exposed to PFOS at different concentrations and time, and the activity of BV-2 cells was detected by MTT assay. The expression of (inducible nitric oxide synthesis,i NOS), IL-6 and (tumor necrosis factor,TNF-偽 m RNA was observed by real-time quantitative PCR, and the expression of kappa B (nuclear factor-kappa B, NF-魏 B and other signal proteins were detected by enzyme-linked immunosorbent assay (Elisa). Results: BV-2 cells were exposed to different concentrations of PFOS for 12 hours, and the cell activity decreased after 24 hours, resulting in obvious cell injury. Elisa results showed that the secretion of inflammatory factor IL-6 increased after PFOS treatment with BV-2 for 24 hours. In addition, PFOS could up-regulate the expression of I NOS,IL-6 gene, but the expression of TNF- 偽 decreased. After incubation with cells, the inflammatory pathway NF- kappa B was significantly activated, showing an increase in the degree of phosphorylation. With the increase of exposure concentration, the expression of p 鈮，

本文編號：2520826