補(bǔ)糖對急性運(yùn)動大鼠心肌單磷酸腺苷活化蛋白激酶激活的影響
發(fā)布時(shí)間：2019-04-30 15:01
【摘要】：目的通過測量大鼠急性運(yùn)動后心肌單磷酸腺苷活化蛋白激酶(AMP-activated protein kinase,AMPK)活性和糖原含量的變化,探討補(bǔ)糖對運(yùn)動心肌AMPK激活的影響。方法大鼠進(jìn)行急性耐力運(yùn)動,并在運(yùn)動前后不同時(shí)間補(bǔ)充不同劑量的補(bǔ)充葡萄糖,采用Western blot測定大鼠心肌AMPK活性的動態(tài)變化,采用蒽酮法測定心肌糖原含量。結(jié)果運(yùn)動誘發(fā)大鼠心肌AMPK活性顯著增高并在急性運(yùn)動后1 h保持在較高水平,然而運(yùn)動補(bǔ)糖大鼠心肌AMPK活性卻無顯著增高。運(yùn)動或小劑量補(bǔ)糖都無法引起大鼠糖原含量的顯著變化,只有大劑量補(bǔ)糖能使糖原含量在運(yùn)動后24 h顯著增高。結(jié)論 (1)急性運(yùn)動可使大鼠心肌AMPK活性升高,補(bǔ)糖能顯著抑制急性運(yùn)動中和運(yùn)動后AMPK的激活。(2)運(yùn)動后大劑量補(bǔ)糖能有效提高運(yùn)動后24 h心肌糖原含量。
[Abstract]:Aim to investigate the effect of glucose supplementation on myocardial AMPK activation by measuring the changes of adenosine monophosphate activated protein kinase (AMP-activated protein kinase,AMPK) activity and glycogen content after acute exercise in rats. Methods the rats were given acute endurance exercise and supplemented with different doses of glucose at different times before and after exercise. The dynamic changes of myocardial AMPK activity were measured by Western blot and the content of myocardial glycogen was measured by anthrone method. Results the activity of myocardial AMPK in exercise-induced rats was significantly increased and maintained at a high level at 1 hour after acute exercise. However, there was no significant increase in myocardial AMPK activity in exercise-supplemented rats. Exercise or low-dose glucose supplementation could not cause significant changes in glycogen content in rats, but high-dose glucose supplementation could significantly increase glycogen content in rats at 24 h after exercise. Conclusion (1) Acute exercise can increase the activity of myocardial AMPK and supplement sugar can significantly inhibit the activation of AMPK after acute exercise. (2) High dose of glucose supplementation after exercise can effectively increase the content of myocardial glycogen 24 hours after exercise.
【作者單位】： 首都體育學(xué)院;
【基金】：北京市教委項(xiàng)目(編號:PXM2015_014206_000040和PXM2015_014206_000072)
【分類號】：R151
本文編號：2468835
[Abstract]:Aim to investigate the effect of glucose supplementation on myocardial AMPK activation by measuring the changes of adenosine monophosphate activated protein kinase (AMP-activated protein kinase,AMPK) activity and glycogen content after acute exercise in rats. Methods the rats were given acute endurance exercise and supplemented with different doses of glucose at different times before and after exercise. The dynamic changes of myocardial AMPK activity were measured by Western blot and the content of myocardial glycogen was measured by anthrone method. Results the activity of myocardial AMPK in exercise-induced rats was significantly increased and maintained at a high level at 1 hour after acute exercise. However, there was no significant increase in myocardial AMPK activity in exercise-supplemented rats. Exercise or low-dose glucose supplementation could not cause significant changes in glycogen content in rats, but high-dose glucose supplementation could significantly increase glycogen content in rats at 24 h after exercise. Conclusion (1) Acute exercise can increase the activity of myocardial AMPK and supplement sugar can significantly inhibit the activation of AMPK after acute exercise. (2) High dose of glucose supplementation after exercise can effectively increase the content of myocardial glycogen 24 hours after exercise.
【作者單位】： 首都體育學(xué)院;
【基金】：北京市教委項(xiàng)目(編號:PXM2015_014206_000040和PXM2015_014206_000072)
【分類號】：R151
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1 趙紀(jì)生;運(yùn)動超1小時(shí)再補(bǔ)糖[N];健康時(shí)報(bào);2006年
2 中國體育科學(xué)學(xué)會 秘書長、研究員 杜利軍 北京康比特運(yùn)動營養(yǎng)研究所 運(yùn)動營養(yǎng)碩士 廉景麗;他們在喝什么？[N];中國體育報(bào);2001年
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