【摘要】：目的研究有機(jī)磷與擬除蟲(chóng)菊酯類(lèi)混配農(nóng)藥對(duì)小鼠的細(xì)胞免疫系統(tǒng)的影響及其機(jī)制。方法以GB 2763—2012《食品安全國(guó)家標(biāo)準(zhǔn)食品中農(nóng)藥最大殘留限量》中規(guī)定的每日允許攝入量為基礎(chǔ),將280只健康SPF級(jí)Balb/c小鼠按體重隨機(jī)分成7組,分別為空白對(duì)照(生理鹽水)組、溶劑對(duì)照(玉米油)組和毒死蜱(0.01 mg/kg)、馬拉硫磷(0.3 mg/kg)、氯氰菊酯(0.02 mg/kg)、高效氯氟氰菊酯(0.02 mg/kg)及混配農(nóng)藥組(0.01 mg/kg毒死蜱+0.3 mg/kg馬拉硫磷+0.02 mg/kg氯氰菊酯+0.02 mg/kg高效氯氟氰菊酯),每組20只,雌雄各半。采用灌胃方式進(jìn)行染毒,每日1次,連續(xù)30 d。測(cè)定小鼠外周血白細(xì)胞、淋巴細(xì)胞、單核細(xì)胞計(jì)數(shù)和脾淋巴細(xì)胞轉(zhuǎn)化功能及外周血T淋巴細(xì)胞亞群以及小鼠血清Th1細(xì)胞因子水平。結(jié)果各染毒組小鼠的脾淋巴細(xì)胞轉(zhuǎn)化功能及白細(xì)胞、淋巴細(xì)胞、單核細(xì)胞水平與溶劑對(duì)照組比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。與溶劑對(duì)照組比較,僅混配農(nóng)藥組雄性小鼠外周血CD8細(xì)胞的比例及血清IL-2、IFN-γ、TNF的水平較低,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);各單一染毒組雄性小鼠外周血CD3、CD4細(xì)胞的比例及CD4/CD8值均無(wú)明顯改變;各染毒組雌性小鼠血清IL-2、IFN-γ、TNF的水平及外周血CD3、CD4、CD8細(xì)胞的比例及CD4/CD8值均無(wú)明顯改變。結(jié)論有機(jī)磷與擬除蟲(chóng)菊酯類(lèi)混配農(nóng)藥對(duì)雄性小鼠具有細(xì)胞免疫毒性作用,其毒性強(qiáng)度高于同等劑量的單一種類(lèi)農(nóng)藥。
[Abstract]:Aim to study the effect and mechanism of organophosphorus and pyrethroid pesticides on cellular immune system in mice. Methods based on the daily allowable intake (ADI) specified in GB 2763 / 2012 National Standard for Food Safety, Balb/c mice of SPF grade were randomly divided into 7 groups according to their body weight. They were blank control (saline) group, solvent control (corn oil) group and chlorpyrifos (0.01 mg/kg), malathion (0.3 mg/kg), cypermethrin (0.02 mg/kg), respectively. High efficiency cyhalothrin (0.02 mg/kg) and mixed agricultural medicine group (0.01 mg/kg chlorpyrifos 0.3 mg/kg malathion 0.02 mg/kg cypermethrin 0.02 mg/kg high efficiency cyhalothrin), 20 rats in each group, half male and female. Oral administration was performed once a day for 30 days. The peripheral blood leukocyte, lymphocyte, monocyte count, spleen lymphocyte transformation function, T lymphocyte subsets in peripheral blood and serum Th1 cytokine level in mice were measured. Results there was no significant difference in splenic lymphocyte transformation function, leukocyte, lymphocyte and monocyte levels between each group and the solvent control group (P0.05). Compared with the solvent control group, the proportion of peripheral blood CD8 cells and the levels of serum IL-2,IFN- 緯 and TNF in the mixed agromedicine group were lower than those in the control group (P0.05). The percentage of CD3,CD4 cells and the value of CD4/ CD8 in peripheral blood of male mice exposed to each single dose group had no significant change. The levels of serum IL-2,IFN- 緯 and TNF, the proportion of CD3,CD4,CD8 cells in peripheral blood and the value of CD4/ CD8 in female mice were not significantly changed in all groups. Conclusion the mixture of organophosphorus and pyrethroids has a cytotoxic effect on male mice, and its toxicity intensity is higher than that of the same dose of single pesticide.
【作者單位】： 天津市疾病預(yù)防控制中心毒理室;
【基金】：天津市衛(wèi)生局科技基金項(xiàng)目(2013KR10)
【分類(lèi)號(hào)】：R114

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