大豆皂甙在小鼠體內(nèi)抗慢性炎癥的活性研究
發(fā)布時(shí)間：2019-02-17 09:46
【摘要】：研究背景慢性炎癥是促進(jìn)慢性非傳染性疾病(NCDs)發(fā)生和發(fā)展的關(guān)鍵因素之一,運(yùn)用膳食來源的具有抗炎活性的植物化學(xué)物來防治NCDs具有重要實(shí)用價(jià)值。大豆皂甙(SS)是來源于大豆及其制品中的一類五環(huán)三萜類植物化學(xué)物,近年來,研究表明SS具有體外抗炎活性。然而,有關(guān)不同SS單體的體內(nèi)抗炎活性鮮見報(bào)道,亟待進(jìn)一步證實(shí)。目的通過建立兩種小鼠體內(nèi)慢性炎癥模型,研究三種SS單體(SS-A1、SS-A2與SS-I)對炎癥標(biāo)記物的調(diào)節(jié)作用,旨在弄清它們在小鼠體內(nèi)的抗慢性炎癥活性,為合理利用SS防治NCDs提供理論依據(jù)。方法(1)將72只ICR雄性小鼠隨機(jī)分為6組(12只/組),對照組3組,注射生理鹽水;處理組3組,尾靜脈間斷(1次/周)注射低劑量脂多糖(LPS),分別處理6周、8周和10周,分析炎癥標(biāo)記物含量以確定建立慢性炎癥模型的處理時(shí)長。(2)將135只ICR雄性小鼠隨機(jī)分為9組(15只/組),對照組注射生理鹽水,第2-9組注射低劑量LPS,共8周,然后分別再用生理鹽水、阿司匹林及不同濃度的SS-A1、SS-A2與SS-I灌胃8周,分析炎癥標(biāo)記物含量。(3)將225只C57BL/6J雄性小鼠隨機(jī)分為對照組(25只)和高脂組(200只),飼喂18周,按體重和肥胖度確定肥胖小鼠,并每組屠宰10只分析炎癥標(biāo)記物含量。(4)將選出的120只肥胖小鼠隨機(jī)分為8組,每組15只,分別飼喂含阿司匹林以及不同濃度的SS-A1、SS-A2及SS-I的高脂飼料8周。分析炎癥標(biāo)記物。(5)數(shù)據(jù)采用SPSS 20.0分析,結(jié)果以x±SD表示。結(jié)果(1)LPS處理8周和10周的ICR小鼠血清或組織(肝、腎、腎周脂肪與睪周脂肪)中的炎癥標(biāo)記物(TNF-α、IL-6、iNOS、COX-2、NO和PGE2)的含量或mRNA表達(dá)量均有不同程度升高(p0.05),我們選擇8周作為ICR小鼠慢性炎癥模型的建立時(shí)長。(2)高脂飼料飼喂18周,按體重和肥胖度的雙重標(biāo)準(zhǔn)選擇120只肥胖小鼠進(jìn)入后續(xù)干預(yù)實(shí)驗(yàn);肥胖小鼠血清或組織中的炎癥標(biāo)記物含量或mRNA表達(dá)量顯著高于對照組小鼠(p0.05)。(3)在兩種小鼠慢性炎癥模型中,阿司匹林可有效降低血清或組織中炎癥標(biāo)記物含量或mRNA表達(dá)量(p0.05);SS-A1、SS-A2與SS-I也可不同程度地降低炎癥標(biāo)記物含量(p0.05),但未發(fā)現(xiàn)濃度依賴效應(yīng),它們還可增加血清中抗氧化酶SOD和GSH-Px的水平(p0.05);SS與阿司匹林的抗炎活性未發(fā)現(xiàn)有規(guī)律的高低差異性。結(jié)論(1)以 TNF-α、IL-1β、IL-6、iNOS/NO、COX-2/PGE2 作為炎癥標(biāo)記物,低劑量LPS間斷尾靜脈注射ICR小鼠8周和高脂飼喂C57BL/6J小鼠18周均可誘導(dǎo)建立體內(nèi)慢性炎癥模型。(2)在兩種小鼠體內(nèi)慢性炎癥模型中,SS-A1、SS-A2和SS-I均可抑制炎癥標(biāo)記物的含量或mRNA表達(dá)量,表明它們在小鼠體內(nèi)具有抗慢性炎癥活性。
[Abstract]:Background chronic inflammation is one of the key factors to promote the occurrence and development of chronic non-communicable disease (NCDs). It is of great practical value to use the anti-inflammatory phytochemicals from dietary sources to prevent and cure NCDs. Soybean saponins (SS) are a class of pentacyclic triterpenoids derived from soybean and its products. In recent years, studies have shown that SS has anti-inflammatory activity in vitro. However, there are few reports about the anti-inflammatory activity of different SS monomers in vivo, which needs to be further confirmed. Objective to study the effects of three kinds of SS monomers (SS-A1,SS-A2 and SS-I) on the regulation of inflammatory markers in mice by establishing two kinds of chronic inflammatory models in order to find out their anti-chronic inflammatory activities in mice. To provide a theoretical basis for the rational use of SS to prevent and cure NCDs. Methods (1) Seventy-two male ICR mice were randomly divided into 6 groups (n = 12) and control group (n = 3). In the treatment group, low dose lipopolysaccharide (LPS),) was injected intermittently (once a week) into caudal vein for 6 weeks, 8 weeks and 10 weeks, respectively. (2) 135 ICR male mice were randomly divided into 9 groups (15 / group), the control group was injected with normal saline, and the 2-9 group was injected with low dose LPS, for 8 weeks. Then they were given saline, aspirin and different concentrations of SS-A1,SS-A2 and SS-I respectively for 8 weeks. (3) 225 male C57BL/6J mice were randomly divided into control group (25) and hyperlipidemia group (200). (4) 120 obese mice were randomly divided into 8 groups, 15 in each group, fed with high fat diet containing aspirin and different concentrations of SS-A1,SS-A2 and SS-I for 8 weeks. (5) the data were analyzed by SPSS 20.0, and the results were expressed as x 鹵SD. Results (1) the inflammatory markers (TNF- 偽, IL-6,iNOS,COX-2,) in serum or tissue (liver, perirenal fat and periorchidism fat) of ICR mice treated with LPS for 8 and 10 weeks. The content of NO and PGE2 or the expression of mRNA increased in varying degrees (p0.05). We chose 8 weeks as the time to establish chronic inflammation model of ICR mice. (2) High fat diet was fed for 18 weeks. According to the double standards of body weight and fat degree, 120 obese mice were selected to participate in the follow-up intervention experiment. The content of inflammatory markers or mRNA expression in serum or tissue of obese mice was significantly higher than that in control mice (p0.05). (3). Aspirin could effectively reduce the content of inflammatory markers or the expression of mRNA in serum or tissue (p0.05). SS-A1,SS-A2 and SS-I also decreased the content of inflammatory markers in varying degrees (p0.05), but there was no concentration-dependent effect. They also increased the levels of antioxidant enzymes SOD and GSH-Px in serum (p0.05). There was no regular difference in anti-inflammatory activity between SS and aspirin. Conclusion (1) TNF- 偽, IL-1 尾, IL-6,iNOS/NO,COX-2/PGE2 were used as inflammatory markers. Low dose LPS intermittent tail vein injection of ICR mice for 8 weeks and hyperlipidemia fed to C57BL/6J mice for 18 weeks could induce chronic inflammation models in vivo. (2) in two kinds of chronic inflammatory models in vivo, SS-A1, was used to induce chronic inflammation in mice. Both SS-A2 and SS-I could inhibit the content of inflammatory markers or the expression of mRNA, indicating that they had anti-chronic inflammatory activity in mice.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R151
[Abstract]:Background chronic inflammation is one of the key factors to promote the occurrence and development of chronic non-communicable disease (NCDs). It is of great practical value to use the anti-inflammatory phytochemicals from dietary sources to prevent and cure NCDs. Soybean saponins (SS) are a class of pentacyclic triterpenoids derived from soybean and its products. In recent years, studies have shown that SS has anti-inflammatory activity in vitro. However, there are few reports about the anti-inflammatory activity of different SS monomers in vivo, which needs to be further confirmed. Objective to study the effects of three kinds of SS monomers (SS-A1,SS-A2 and SS-I) on the regulation of inflammatory markers in mice by establishing two kinds of chronic inflammatory models in order to find out their anti-chronic inflammatory activities in mice. To provide a theoretical basis for the rational use of SS to prevent and cure NCDs. Methods (1) Seventy-two male ICR mice were randomly divided into 6 groups (n = 12) and control group (n = 3). In the treatment group, low dose lipopolysaccharide (LPS),) was injected intermittently (once a week) into caudal vein for 6 weeks, 8 weeks and 10 weeks, respectively. (2) 135 ICR male mice were randomly divided into 9 groups (15 / group), the control group was injected with normal saline, and the 2-9 group was injected with low dose LPS, for 8 weeks. Then they were given saline, aspirin and different concentrations of SS-A1,SS-A2 and SS-I respectively for 8 weeks. (3) 225 male C57BL/6J mice were randomly divided into control group (25) and hyperlipidemia group (200). (4) 120 obese mice were randomly divided into 8 groups, 15 in each group, fed with high fat diet containing aspirin and different concentrations of SS-A1,SS-A2 and SS-I for 8 weeks. (5) the data were analyzed by SPSS 20.0, and the results were expressed as x 鹵SD. Results (1) the inflammatory markers (TNF- 偽, IL-6,iNOS,COX-2,) in serum or tissue (liver, perirenal fat and periorchidism fat) of ICR mice treated with LPS for 8 and 10 weeks. The content of NO and PGE2 or the expression of mRNA increased in varying degrees (p0.05). We chose 8 weeks as the time to establish chronic inflammation model of ICR mice. (2) High fat diet was fed for 18 weeks. According to the double standards of body weight and fat degree, 120 obese mice were selected to participate in the follow-up intervention experiment. The content of inflammatory markers or mRNA expression in serum or tissue of obese mice was significantly higher than that in control mice (p0.05). (3). Aspirin could effectively reduce the content of inflammatory markers or the expression of mRNA in serum or tissue (p0.05). SS-A1,SS-A2 and SS-I also decreased the content of inflammatory markers in varying degrees (p0.05), but there was no concentration-dependent effect. They also increased the levels of antioxidant enzymes SOD and GSH-Px in serum (p0.05). There was no regular difference in anti-inflammatory activity between SS and aspirin. Conclusion (1) TNF- 偽, IL-1 尾, IL-6,iNOS/NO,COX-2/PGE2 were used as inflammatory markers. Low dose LPS intermittent tail vein injection of ICR mice for 8 weeks and hyperlipidemia fed to C57BL/6J mice for 18 weeks could induce chronic inflammation models in vivo. (2) in two kinds of chronic inflammatory models in vivo, SS-A1, was used to induce chronic inflammation in mice. Both SS-A2 and SS-I could inhibit the content of inflammatory markers or the expression of mRNA, indicating that they had anti-chronic inflammatory activity in mice.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R151
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