【摘要】：目的探討深海魚油對大氣細顆粒物(PM2.5)引起的心血管系統(tǒng)中的炎性損傷和氧化應(yīng)激損傷影響。方法將SD雄性大鼠隨機分為魚油低、中、高劑量組,魚油對照組,溶劑對照組(玉米油)和PM2.5染毒組;魚油組大鼠先進行深海魚油灌胃28 d,再進行PM2.5氣管滴注染毒隔天1次,共3次,PM2.5染毒期間同時進行魚油灌胃,PM2.5染毒組先正常飼養(yǎng)28 d,再進行PM2.5染毒;取血清檢測白細胞介素-6(IL-6)、腫瘤壞死因子α(TNF-α)、超敏C反應(yīng)蛋白(hs-CRP)、丙二醛(MDA)、總超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)和谷胱甘肽過氧化物酶(GSH-Px)。結(jié)果與溶劑對照組比較,PM2.5染毒組大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分別為(24.94±2.06)、(821.45±14.26)ng/m L、(3.10±0.28)mg/L、(15.88±1.41)nmol/m L]升高,GSH、GSH-Px、TSOD活性[(4.62±0.37)μmol/L、(289.28±30.65)活力單位、(239.26±4.97)U/m L]降低(均P0.05);與PM2.5染毒組比較,魚油高劑量組大鼠血清中IL-6、TNF-α、hs-CRP、MDA含量[分別為(16.67±0.23)、(73.01±3.80)ng/m L、(1.36±0.22)mg/L、(11.82±0.79)nmol/m L]下降,GSH、GSH-Px、T-SOD活性[(24.05±3.83)μmol/L、(531.33±40.39)活力單位、(273.36±3.92)U/m L]升高,呈劑量效應(yīng)關(guān)系(均P0.05)。結(jié)論深海魚油對大氣PM2.5所致心血管系統(tǒng)炎性反應(yīng)和氧化應(yīng)激損傷具有明顯拮抗作用。
[Abstract]:Objective to investigate the effects of deep-sea fish oil on inflammatory injury and oxidative stress injury in cardiovascular system induced by atmospheric fine particulate matter (PM2.5). Methods male SD rats were randomly divided into low, medium and high dose groups, fish oil control group, solvent control group (corn oil) and PM2.5 exposure group. The rats in the fish oil group were fed with deep-sea fish oil for 28 days and then injected with PM2.5 once every other day for 3 times. The rats in the PM2.5 group were fed with fish oil at the same time during PM2.5 exposure. The rats in the PM2.5 group were fed normally for 28 days and then PM2.5. Serum levels of interleukin-6 (IL-6), tumor necrosis factor 偽 (TNF- 偽), hypersensitive C-reactive protein (hs-CRP) and malondialdehyde (MDA),) total superoxide dismutase (T-SOD) were measured. Glutathione (GSH) and glutathione peroxidase (GSH-Px). Results compared with solvent control group, the contents of IL-6,TNF- 偽 and hs-CRP,MDA in serum of rats exposed to PM2.5 were (24.94 鹵2.06), (821.45 鹵14.26) ng/m L, (3.10 鹵0.28) mg/L, respectively. The activity of GSH,GSH-Px,TSOD [(4.62 鹵0.37) 渭 mol/L, (289.28 鹵30.65), (239.26 鹵4.97) U / mL] was increased (P 0.05). Compared with PM2.5 exposure group, the contents of IL-6,TNF- 偽 and hs-CRP,MDA in serum of high dose group of fish oil were (16.67 鹵0.23), (73.01 鹵3.80) ng/m L, (1.36 鹵0.22) mg/L, respectively. The activity of GSH,GSH-Px,T-SOD [(24.05 鹵3.83) 渭 mol/L, (531.33 鹵40.39), (273.36 鹵3.92) U / mL] decreased, and the activity of GSH,GSH-Px,T-SOD increased. There was a dose-effect relationship (P0.05). Conclusion Deep-sea fish oil has obvious antagonistic effect on cardiovascular system inflammatory response and oxidative stress injury induced by atmospheric PM2.5.
【作者單位】： 復(fù)旦大學(xué)公共衛(wèi)生學(xué)院公共衛(wèi)生安全教育部重點實驗室;
【基金】：國家自然科學(xué)基金(81172617)
【分類號】：R151

【參考文獻】

相關(guān)期刊論文 前6條

1 閆慶倩;趙學(xué)彬;楊莉;彭曉武;王正倫;楊磊;;不同地區(qū)大氣PM2.5致大鼠肺損傷的比較實驗研究[J];環(huán)境與健康雜志;2012年01期

2 張文麗;空氣細顆粒物(PM2.5)生物效應(yīng)指標(biāo)研究進展[J];衛(wèi)生研究;2001年06期

3 扶志敏;王正;;ω-3多不飽和脂肪酸抗炎機制研究進展[J];醫(yī)藥導(dǎo)報;2009年09期

4 肖純凌;李舒音;尚德志;朱小敏;陳冬梅;王任群;;PM_(2.5)大氣污染物致大鼠呼吸系統(tǒng)病理學(xué)變化[J];中國公共衛(wèi)生;2011年12期

5 姜淑卿;李大鳴;張靜姝;;深海魚油中主要生理活性成分影響認知功能的研究進展[J];中國慢性病預(yù)防與控制;2012年02期

6 李娟;楊維超;洪麗娟;姚武;吳衛(wèi)東;吳逸明;燕貞;;PM_(2.5)對BEAS-2B細胞脂質(zhì)過氧化損傷作用[J];中國公共衛(wèi)生;2014年11期

【共引文獻】

相關(guān)期刊論文 前10條

1 楊嵩;張文波;張寶;唐白云;;ω-3多不飽和脂肪酸對冠狀動脈旁路移植術(shù)后炎癥反應(yīng)的影響[J];廣東醫(yī)學(xué);2011年17期

2 劉潔嶺;蔣文舉;;PM_(2.5)的研究現(xiàn)狀及防控對策[J];廣州化工;2012年23期

3 劉朝軍;王熙;張潔;沈建昌;;高容塵空氣過濾材料及其器件的開發(fā)[J];過濾與分離;2014年01期

4 胡潔;臧紅霞;;華北地區(qū)PM2.5的污染特征及來源分析[J];廣東化工;2014年16期

5 朱可s，

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