【摘要】：分析芹菜素(apigenin,AP)對(duì)丙烯腈(acrylonitrile,ACN)引起的大鼠精子脂質(zhì)過氧化和DNA損傷的影響,并探討其可能的機(jī)制。將50只SPF級(jí)SD成年雄性大鼠隨機(jī)分為陰性對(duì)照組(玉米油)、ACN組(50 mg·kg~(-1)ACN)、低AP組(50 mg·kg~(-1)ACN+234 mg·kg~(-1)AP)、高AP組(50 mg·kg~(-1)ACN+468 mg·kg~(-1)AP)、N-乙酰半胱氨酸(N-acetylcysteine,NAC)組(50 mg·kg~(-1)ACN+300mg·kg~(-1)NAC),以5 m L·(kg bw)~(-1)灌胃染毒,1次·d~(-1),6 d·周~(-1),連續(xù)13周。檢測(cè)大鼠精子活性氧(ROS)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及精子DNA損傷情況。結(jié)果發(fā)現(xiàn),ACN組、低AP組、高AP組、NAC組精子ROS、MDA含量顯著升高,SOD活力顯著降低,精子尾部DNA含量百分比、尾長(zhǎng)、尾距、Olive尾距均顯著增高于對(duì)照組(均P0.05);而低AP組、高AP組、NAC組精子ROS、MDA含量、SOD活性和精子DNA損傷情況與ACN組相比差異均無統(tǒng)計(jì)學(xué)意義(P0.05)。提示ACN可引起大鼠精子脂質(zhì)過氧化和DNA損傷,而AP、NAC對(duì)其無干預(yù)作用。
[Abstract]:The effects of apigenin (apigenin,AP) on lipid peroxidation and DNA damage in rat sperm induced by acrylonitrile (acrylonitrile,ACN) were analyzed and its possible mechanism was discussed. Fifty SPF grade SD adult male rats were randomly divided into negative control group (50 mg kg~ (-1) ACN), low AP) ACN 234 mg kg~ (-1) AP),) group (50 mg kg~ (-1) ACN), low AP group). High AP group (50 mg kg~ (-1) ACN 468 mg kg~ (-1) AP), N-acetylcysteine NAC) group (50 mg kg~ (-1) ACN 300mg kg~ (-1) NAC),) 5 m L (kg bw) ~ (-1) was administered intragastrically, once for d ~ (-1), 6 d ~ (-1) for 13 weeks. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and the damage of sperm DNA were measured. The results showed that in ACN group, low AP group, high AP group, NAC group, the content of ROS,MDA in spermatozoa increased significantly, and the activity of SOD decreased significantly. The percentage of DNA content, tail length, tail distance and Olive tail distance in ACN group, low AP group, high AP group and NAC group were significantly higher than those in control group (P0.05). However, there was no significant difference between the low AP group, the high AP group and the NAC group in the content of ROS,MDA, the activity of SOD and the damage of DNA in the sperm compared with the ACN group (P0.05). These results suggest that ACN can induce lipid peroxidation and DNA damage in rat spermatozoa, but AP,NAC has no effect on it.
【作者單位】： 蘭州大學(xué)公共衛(wèi)生學(xué)院;
【基金】：中央高校基本科研業(yè)務(wù)專項(xiàng)資金項(xiàng)目(lzujbky-2014-221)
【分類號(hào)】：R151

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 高娟;賈青;楊博;劉麗均;郁麗麗;芮榮;徐平;;大鼠精子冷凍技術(shù)研究進(jìn)展[J];實(shí)驗(yàn)動(dòng)物與比較醫(yī)學(xué);2008年03期

2 張皓;鄭唯椺;王霞;劉莉;屈衛(wèi)東;;S型氯代甘油醇對(duì)大鼠精子運(yùn)動(dòng)和超活化抑制作用研究[J];衛(wèi)生研究;2012年05期

3 才秀蓮;郭海;李豐;;錳對(duì)大鼠精子形態(tài)的影響及意義[J];遵義醫(yī)學(xué)院學(xué)報(bào);2009年06期

4 余傳林，單春文，，張家新，孫莉莎，駱蘇芳，楊景琴，陳興堅(jiān)，樊紅鷹;亞硒酸鈉對(duì)雄性大鼠精子生成數(shù)的影響[J];第一軍醫(yī)大學(xué)學(xué)報(bào);1994年01期

5 江俊康,翁詩君;鉛與乙醇對(duì)大鼠精子的聯(lián)合毒性[J];中國工業(yè)醫(yī)學(xué)雜志;2005年02期

6 張華,盧國s

本文編號(hào)：2333623