【摘要】：根據(jù)羊cyt B基因設(shè)計3對特異性引物,運用GenieⅡ等溫擴增熒光檢測系統(tǒng),以LAMP熒光檢測方法為基礎(chǔ),建立了檢測羊源性成分的LAMP方法,并對該方法進行了反應(yīng)體系和條件的優(yōu)化。試驗結(jié)果表明:該方法操作簡單、特異性好;靈敏度比普通PCR方法高100倍,比q PCR方法低10倍,達到5.928×10-3μg/μL;反應(yīng)時間短,最快10 min即可完成反應(yīng)。
[Abstract]:Three pairs of specific primers were designed according to sheep cyt B gene. The LAMP method for detection of goat derived components was established by using Genie 鈪，

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