【摘要】：建立HPLC法測定寶塔菜中的水蘇糖含量。采用島津2010AHT色譜儀,菲羅門NH2色譜柱(5.0mm×150mm,4.6μm);流動相乙腈-水(67:33);柱溫為30℃,流速為1.0ml/min。以水蘇糖進(jìn)樣量X為橫坐標(biāo),峰面積Y為縱坐標(biāo),求得回歸方程:Y=10381X+9888.8,水蘇糖在5.004μg至400.32μg范圍內(nèi)線性關(guān)系良好(R2=0.9993),平均回收率為99.68%,RSD為0.42%。該方法結(jié)果準(zhǔn)確,可作為寶塔菜中水蘇糖含量的測定方法。
[Abstract]:A HPLC method was established for the determination of threonose in Pagoda. A 2010AHT chromatograph of Shimadzu was used. The mobile phase of acetonitrile-water (67:33) was performed on a column of 5.0mm 脳 150mm ~ (4.6) 渭 m);, the column temperature was 30 鈩，

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