【摘要】：目的探討一次性尾靜脈注射納米氧化石墨烯顆粒(GO)對小鼠心肌ATP酶、LDH、AST活性影響,為其在生物學領域的安全應用提供科學依據(jù)。方法選擇80只清潔級ICR小鼠,體重(18±2)g,雌雄各半,隨機分為4組,分別以劑量為1.40、0.70、0.35 mg/kg氧化石墨烯對小鼠一次性尾靜脈注射,對照組注射蒸餾水。并于注射后3 d和15 d將小鼠脫臼處死,取心臟,測定心肌勻漿液中的Na~+,K~+-ATP酶、Ca~(2+),Mg~(2+)-ATP酶、LDH、AST活性測定。結(jié)果注射后3 d,雌、雄中、高劑量Na~+,K~+-ATP酶活性及Ca~(2+),Mg~(2+)-ATP酶活性均高于對照組(P0.05)。15 d后,雌、雄各劑量組Na~+,K~+-ATP酶及Ca~(2+),Mg~(2+)-ATP酶活性均低于對照組(P0.05)。注射后3 d,雌、雄各組間AST活性無統(tǒng)計學意義(P0.05),15 d后,雌、雄各劑量GO組明顯低于對照組(P0.05)。注射3 d,雄性各劑量GO組LDH活性明顯低于對照組(P0.05),雌性高劑量GO組明顯低于對照組和低劑量GO組。注射后15 d,雄性高劑量GO組顯著低于中、低劑量GO組(P0.05),雌性中、高劑GO量組明顯低于對照、低劑量GO組(P0.05)。結(jié)論一次性尾靜脈注射氧化石墨烯可干擾Na~+,K~+-ATP酶、Ca~(2+),Mg~(2+)-ATP酶和AST、LDH等心肌酶活性的作用。
[Abstract]:Objective to investigate the effect of single tail vein injection of nano-graphene oxide granule (GO) on the activity of ATP and LDH,AST in myocardium of mice, and to provide scientific basis for its safe application in biological field. Methods Eighty clean grade ICR mice, weighing (18 鹵2) g, half male and half female, were randomly divided into 4 groups. The mice were injected with graphene oxide at a dose of 1.40U 0.700.35 mg/kg via a single tail vein, while the control group was injected with distilled water. The mice were killed 3 and 15 days after injection, and the Na~, K- ATP, Ca~ (2), Mg~ (2)-ATP and LDH,AST activities in myocardial homogenate were determined. Results the activities of Na~, K- ATP, Ca~ (2), Mg~ (2)-ATP in female and male were higher than those in control group 3 days after injection (P0.05). 15 days after injection, Na~, K- ATP enzyme and Ca~ (2) in female and male groups were higher than those in control group. The activity of Mg~ (2)-ATP was lower than that of control group (P0.05). There was no significant difference in AST activity between female and male groups at 3 d after injection (P0.05). 15 days later, the activity of GO in female and male groups was significantly lower than that in control group (P0.05). The LDH activity of male GO group was significantly lower than that of control group (P0.05), and that of female high dose GO group was significantly lower than that of control group and low dose GO group. 15 days after injection, male high dose GO group was significantly lower than middle dose group, low dose GO group (P0.05), female, high dose GO group was significantly lower than control group, low dose GO group (P0.05). Conclusion single tail vein injection of graphene oxide can interfere with the activities of myocardial enzymes such as Na~, K- ATP, Ca~ (2), Mg~ (2)-ATP and AST,LDH.
【作者單位】： 內(nèi)蒙古醫(yī)科大學公共衛(wèi)生學院環(huán)境與職業(yè)衛(wèi)生學教研室;吉林大學公共衛(wèi)生學院環(huán)境衛(wèi)生與勞動衛(wèi)生學教研室;
【分類號】：R114

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