青島城鄉(xiāng)居民葉黃素?cái)z入調(diào)查及吸收代謝研究
發(fā)布時(shí)間：2018-10-30 17:32
【摘要】：目的:為指導(dǎo)居民合理膳食攝入葉黃素及科學(xué)補(bǔ)充葉黃素,現(xiàn)對(duì)青島市城鄉(xiāng)居民日常膳食葉黃素?cái)z入情況進(jìn)行調(diào)查并對(duì)葉黃素在青年體內(nèi)的吸收代謝狀況進(jìn)行試驗(yàn)研究。方法:按照類型抽樣的方法,隨機(jī)抽取青島市市北區(qū)某社區(qū)(城市)和青島市即墨市某自然村(農(nóng)村)為調(diào)查點(diǎn),自2015年1月至2016年1月,由經(jīng)培訓(xùn)的專業(yè)調(diào)查員進(jìn)行基本情況調(diào)查、3天24小時(shí)膳食回顧調(diào)查,采用“知情同意”的原則,共調(diào)查居民308名(城市居民198名,農(nóng)村居民110名),其中青年(18歲-44歲)89名,中年(45歲-59歲)124名,老年(60歲以上)95名。調(diào)查者詳細(xì)詢問(wèn)并填寫調(diào)查問(wèn)卷,記錄居民性別、年齡、身高、體重、學(xué)歷、月收入、身體健康狀況等基本情況,保證基本信息的完整性。采用問(wèn)答的方式參考膳食圖譜調(diào)查近期連續(xù)三天的飲食情況(避免節(jié)假日或非常規(guī)聚餐),將調(diào)查結(jié)果進(jìn)行雙錄入統(tǒng)計(jì)分析。隨機(jī)選取青島市某高校20名健康青年志愿者,隨機(jī)分為葉黃素組和正常對(duì)照組,葉黃素組每天補(bǔ)充葉黃素20毫克,連續(xù)補(bǔ)充20天,正常對(duì)照組未給予任何補(bǔ)充。試驗(yàn)前(0小時(shí))及首次口服后的12小時(shí),24小時(shí),72小時(shí),144小時(shí),240小時(shí),480小時(shí),720小時(shí)分別留取清晨空腹靜脈血5毫升,分離血漿。HPLC檢測(cè)血漿葉黃素濃度。采用SPSS17.0統(tǒng)計(jì)軟件對(duì)數(shù)據(jù)進(jìn)行錄入分析,檢驗(yàn)水準(zhǔn)α=0.05。結(jié)果:城鄉(xiāng)居民膳食葉黃素?cái)z入量的中位數(shù)為2709μg/d,農(nóng)村居民葉黃素?cái)z入量顯著高于城市(P0.05),且農(nóng)村男女居民葉黃素?cái)z入量分別顯著高于城市男女居民(P0.05)。蔬菜是青島市城鄉(xiāng)居民膳食葉黃素的主要來(lái)源,所占比重高達(dá)95%,其中菠菜、韭菜、小白菜是城鄉(xiāng)居民膳食葉黃素的最主要來(lái)源。農(nóng)村居民豆類及水果中葉黃素?cái)z入量顯著低于城市居民(P0.05),城市居民糧谷類及蔬菜中葉黃素?cái)z入量顯著低于農(nóng)村居民(P0.05)。中年組居民膳食葉黃素?cái)z入量顯著高于青年組及老年組居民(P0.05)。青年組通過(guò)水果類、奶制品攝入的葉黃素顯著高于中年組及老年組(P0.05);中年組通過(guò)糧谷類、蔬菜類攝入的葉黃素顯著高于青年組及老年組(P0.05),中年組通過(guò)蛋類攝入的葉黃素顯著高于老年組(P0.05);老年組通過(guò)豆類攝入的葉黃素顯著高于青年組及中年組(P0.05)。在葉黃素的吸收代謝研究中,20名青年志愿者的試驗(yàn)前血漿葉黃素本底水平為0.361±0.095μg/m L,隨著葉黃素膠囊的持續(xù)性補(bǔ)充,志愿者的血漿葉黃素濃度呈穩(wěn)定性上升趨勢(shì),連續(xù)服用葉黃素膠囊20天后,葉黃素組血漿葉黃素濃度為1.112±0.559μg/m L,與補(bǔ)充前(0小時(shí))相比,葉黃素組血漿葉黃素濃度在第一次口服膠囊后的72小時(shí),144小時(shí),240小時(shí),480小時(shí)分別升高59%,115%,164%,214%(P均0.05)。結(jié)論:青島市城鄉(xiāng)居民每日膳食葉黃素?cái)z入量的中位數(shù)為2709μg,農(nóng)村居民每日膳食葉黃素?cái)z入量高于城市居民,中年居民每日膳食葉黃素?cái)z入量高于青年及老年居民。城市居民水果及豆類中葉黃素?cái)z入量較高,農(nóng)村居民糧谷類及蔬菜中葉黃素?cái)z入量較高;青年組居民水果及奶制品中葉黃素?cái)z入量較高,中年組居民蛋類、蔬菜及糧谷類中葉黃素?cái)z入量較高,老年組居民豆類中葉黃素?cái)z入量較高。來(lái)源于蔬菜的葉黃素?cái)z入量達(dá)95%,菠菜、韭菜、小白菜是居民膳食葉黃素的最主要來(lái)源。連續(xù)口服葉黃素膠囊,血漿葉黃素水平呈穩(wěn)定上升趨勢(shì),口服20天后,血漿葉黃素水平升高為1.112μg/m L,是口服前的3.28倍。
[Abstract]:Objective: To investigate the daily dietary lutein intake of urban and rural residents in Qingdao, and to study the absorption and metabolism of lutein in young people. Methods: According to the method of sampling, a community (city) of Qingdao City and a natural village (rural) in Qingdao, Jimo City, were randomly selected as an investigation point, from January 2015 to January 2016. Basic information surveys conducted by trained professional investigators, a 3-day 24-hour diet review survey, Informed Consent According to the principle, 308 residents (198 urban residents and 110 rural residents) were investigated, among which 89 were young (18-44 years old), 124 in middle-aged (45-59) and 95 in old age (over 60 years). The investigator asked and filled out the questionnaire to record the basic information such as sex, age, height, weight, educational background, monthly income and health status of the residents, so as to ensure the integrity of basic information. Use a question-and-answer mode to reference the diet map to investigate the recent three-day diet (avoid holidays or irregular holidays), and double-entry statistical analysis of the results. Twenty healthy young volunteers were randomly divided into Lutein group and normal control group. The lutein group was supplemented with 20 mg lutein daily for 20 days and no supplementation was given to normal control group. Before the test (0 hours) and 12 hours, 24 hours, 72 hours, 144 hours, 240 hours, 480 hours and 720 hours after the first oral administration, 5 ml of fasting venous blood were taken for 720 hours, respectively, and the plasma was separated. Plasma lutein concentration was detected by HPLC. The data were entered and analyzed using SPSS 17. 0 statistical software, and the inspection level was 0. 05. Results: The median of lutein intake of urban and rural residents was 2709 ug/ d, and the intake of lutein in rural residents was significantly higher than that in cities (P0.05). Vegetable is the main source of dietary lutein from urban and rural residents in Qingdao, accounting for 95% of the proportion, among which spinach, leek and Chinese cabbage are the most important sources of dietary lutein from urban and rural residents. The intake of lutein in beans and fruits of rural residents was significantly lower than that of urban residents (P0.05). The intake of dietary lutein in middle-aged group was significantly higher than that of young group and elderly group (P0.05). The xanthophyll intake of the young group was significantly higher than that in the middle-aged group and the elderly group (P <0.05). The lutein content of the middle-aged group was significantly higher than that in the old group and the old group (P0.05). Lutein intake was significantly higher in the aged group than in the young group and middle-aged group (P0.05). In the study of the absorption and metabolism of lutein, the background level of lutein in the plasma of 20 young volunteers was 0. 361 bb0. 095 ug/ m L. With the continuous replenishment of lutein capsule, the plasma lutein concentration of volunteers increased steadily, and the lutein capsule was administered continuously for 20 days. The concentration of lutein in lutein group was 1. 112% 0. 559 ug/ m L. Compared with the supplementation (0 h), the concentration of lutein in lutein group increased 59%, 115%, 164%, 214% (P 0.05) in 72 hours, 144 hours, 240 hours and 480 hours after the first oral capsule. Conclusion: The median dietary lutein intake of urban and rural residents in Qingdao is 2709 碌g, and the daily dietary lutein intake of rural residents is higher than that of urban residents. The daily dietary lutein intake of middle-aged residents is higher than that of young people and elderly residents. The intake of lutein in the fruits and beans of urban residents is higher, the intake of lutein in grain and vegetables of rural residents is higher, the intake of lutein in fruit and dairy products of young people is higher, and the intake of lutein in the middle-aged group, the eggs, vegetables and grains is higher, The intake of lutein in legumes in the aged group was higher. The intake of lutein from vegetables reached 95%, spinach, leek and cabbage were the main sources of dietary lutein. After 20 days after oral administration, the level of lutein in plasma increased to 1. 112 ug/ m L, which was 3. 28 times before oral administration.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R151.42
本文編號(hào)：2300690
[Abstract]:Objective: To investigate the daily dietary lutein intake of urban and rural residents in Qingdao, and to study the absorption and metabolism of lutein in young people. Methods: According to the method of sampling, a community (city) of Qingdao City and a natural village (rural) in Qingdao, Jimo City, were randomly selected as an investigation point, from January 2015 to January 2016. Basic information surveys conducted by trained professional investigators, a 3-day 24-hour diet review survey, Informed Consent According to the principle, 308 residents (198 urban residents and 110 rural residents) were investigated, among which 89 were young (18-44 years old), 124 in middle-aged (45-59) and 95 in old age (over 60 years). The investigator asked and filled out the questionnaire to record the basic information such as sex, age, height, weight, educational background, monthly income and health status of the residents, so as to ensure the integrity of basic information. Use a question-and-answer mode to reference the diet map to investigate the recent three-day diet (avoid holidays or irregular holidays), and double-entry statistical analysis of the results. Twenty healthy young volunteers were randomly divided into Lutein group and normal control group. The lutein group was supplemented with 20 mg lutein daily for 20 days and no supplementation was given to normal control group. Before the test (0 hours) and 12 hours, 24 hours, 72 hours, 144 hours, 240 hours, 480 hours and 720 hours after the first oral administration, 5 ml of fasting venous blood were taken for 720 hours, respectively, and the plasma was separated. Plasma lutein concentration was detected by HPLC. The data were entered and analyzed using SPSS 17. 0 statistical software, and the inspection level was 0. 05. Results: The median of lutein intake of urban and rural residents was 2709 ug/ d, and the intake of lutein in rural residents was significantly higher than that in cities (P0.05). Vegetable is the main source of dietary lutein from urban and rural residents in Qingdao, accounting for 95% of the proportion, among which spinach, leek and Chinese cabbage are the most important sources of dietary lutein from urban and rural residents. The intake of lutein in beans and fruits of rural residents was significantly lower than that of urban residents (P0.05). The intake of dietary lutein in middle-aged group was significantly higher than that of young group and elderly group (P0.05). The xanthophyll intake of the young group was significantly higher than that in the middle-aged group and the elderly group (P <0.05). The lutein content of the middle-aged group was significantly higher than that in the old group and the old group (P0.05). Lutein intake was significantly higher in the aged group than in the young group and middle-aged group (P0.05). In the study of the absorption and metabolism of lutein, the background level of lutein in the plasma of 20 young volunteers was 0. 361 bb0. 095 ug/ m L. With the continuous replenishment of lutein capsule, the plasma lutein concentration of volunteers increased steadily, and the lutein capsule was administered continuously for 20 days. The concentration of lutein in lutein group was 1. 112% 0. 559 ug/ m L. Compared with the supplementation (0 h), the concentration of lutein in lutein group increased 59%, 115%, 164%, 214% (P 0.05) in 72 hours, 144 hours, 240 hours and 480 hours after the first oral capsule. Conclusion: The median dietary lutein intake of urban and rural residents in Qingdao is 2709 碌g, and the daily dietary lutein intake of rural residents is higher than that of urban residents. The daily dietary lutein intake of middle-aged residents is higher than that of young people and elderly residents. The intake of lutein in the fruits and beans of urban residents is higher, the intake of lutein in grain and vegetables of rural residents is higher, the intake of lutein in fruit and dairy products of young people is higher, and the intake of lutein in the middle-aged group, the eggs, vegetables and grains is higher, The intake of lutein in legumes in the aged group was higher. The intake of lutein from vegetables reached 95%, spinach, leek and cabbage were the main sources of dietary lutein. After 20 days after oral administration, the level of lutein in plasma increased to 1. 112 ug/ m L, which was 3. 28 times before oral administration.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R151.42
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