【摘要】：目的研究生活飲用水中損傷性非苛養(yǎng)菌的耐藥性。方法采用增菌培養(yǎng)基和選擇性培養(yǎng)基對某小區(qū)生活飲用水進(jìn)行損傷菌分離,獲得分離株后,利用16S rRNA基因測序法進(jìn)行種屬鑒定;采用Kirby-Bauer紙片擴(kuò)散法進(jìn)行細(xì)菌的抗菌藥物敏感性試驗(yàn)。結(jié)果在分離的58株損傷性非苛養(yǎng)菌中,耐藥菌占51.72%,包括腸球菌屬、腸桿菌屬、葡萄球菌屬、不動(dòng)桿菌屬、假單胞菌屬。其中80%的損傷菌表現(xiàn)出對兩種及以上抗菌藥物耐藥。耐藥菌對復(fù)方新諾明的耐藥最為普遍,耐藥率為63.33%;對磺胺甲VA唑的耐藥次之,占43.33%。結(jié)論測試小區(qū)生活飲用水中的損傷性非苛養(yǎng)菌耐藥性較為普遍,應(yīng)加強(qiáng)關(guān)注其潛在的人群健康風(fēng)險(xiǎn)。
[Abstract]:Objective to study the drug resistance of damaged non-culture bacteria in drinking water. Methods Bacterization medium and selective medium were used to isolate the damaged bacteria from drinking water in a small area. The isolated strains were identified by 16s rRNA gene sequencing method. The antimicrobial susceptibility of bacteria was tested by Kirby-Bauer disk diffusion method. Results among the 58 strains of non-caustic injurious bacteria, drug-resistant bacteria accounted for 51.72%, including Enterococcus, Enterobacter, Staphylococcus, Acinetobacter, Pseudomonas. 80% of them showed resistance to two or more antimicrobial agents. The resistance of drug-resistant bacteria to compound sulfamethoxazole was the most common, the resistance rate was 63.33, and the resistance to sulfamethoxazole was the second, accounting for 43.33%. Conclusion the drug resistance of damaged non-culture bacteria in drinking water in residential areas is more common, and attention should be paid to the potential population health risks.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院衛(wèi)生學(xué)環(huán)境醫(yī)學(xué)研究所;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81372947)
【分類號(hào)】：R123.1

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