【摘要】：目的對水產(chǎn)品牡蠣污染耐藥性細菌進行調(diào)查,并檢測其攜帶整合子類型,為細菌耐藥機制研究提供依據(jù)。方法利用抗性培養(yǎng)基平板篩選牡蠣標(biāo)本中的耐藥性細菌,用K-B法進行藥敏試驗,PCR法擴增Ⅰ型、Ⅱ型和Ⅲ型整合酶基因。結(jié)果 34份牡蠣標(biāo)本分離出76株耐藥株,其中超過90%的分離菌對四環(huán)素、氨芐西林耐藥,諾氟沙星、左氧氟沙星、復(fù)方新諾明、環(huán)丙沙星、頭孢唑啉和氯霉素耐藥50%,有2株對耐亞胺培南細菌。整合酶基因檢測結(jié)果顯示80.26%耐藥細菌攜帶Ⅰ型整合子,其中6株同時攜帶Ⅱ型整合子,未檢測到Ⅲ型整合子。所有攜帶整合子的耐藥細菌是多重耐藥,不攜帶整合子耐藥細菌中多重耐藥占60%。結(jié)論牡蠣污染細菌的耐藥性嚴重,且多重耐藥與攜帶整合子有關(guān),攜帶整合子細菌更易產(chǎn)生多重耐藥,提示整合子可作為食品分離菌的耐藥性檢測靶標(biāo)。
[Abstract]:Objective To investigate the drug-resistant bacteria of oyster contaminated with aquatic products and to detect the type of drug-resistant bacteria and provide the basis for the study of the mechanism of bacterial resistance. Methods The resistant bacteria in oyster samples were screened by using resistant culture medium plate. The susceptibility test was carried out by K-B method. PCR method was used to amplify type 鈪，

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