不同劑量果糖攝入對孕期大鼠糖代謝影響的研究
發(fā)布時間：2018-10-14 12:11
【摘要】：目的:給予孕期大鼠不同劑量果糖灌胃,觀察孕期大鼠血糖、胰島素水平及胚胎發(fā)育情況,比較不同劑量果糖攝入對孕期大鼠糖代謝的影響,初步探討高果糖攝入致孕期大鼠糖代謝異常的機制。方法:成年雌性與雄性SD大鼠適應(yīng)性喂養(yǎng)1周,以2:1比例合籠交配,次日晨做陰道涂片,顯微鏡下檢查發(fā)現(xiàn)精子者記孕第0天,將48只孕鼠隨機分為4組,即空白對照組(BC)、正常劑量果糖組(NF)、高劑量果糖組(HF)、超高劑量果糖組(SHF),分別給蒸餾水、2.0g/kg、5.0g/kg和8.0g/kg果糖各1ml灌胃,于妊娠第18天行口服葡萄糖耐量試驗(OGTT),孕第20天處死。1)每日記錄攝食量,每周稱量體重并比較各組進食量及體重增長情況;2)孕第18天行OGTT檢查,評估糖耐量;3)檢測空腹胰島素水平,計算胰島素敏感指數(shù)和胰島素抵抗指數(shù),評估胰島功能;4)留取胰腺組織蘇木精-伊紅染色法觀察其組織形態(tài)學(xué)的變化;5)剖腹取胎鼠,觀察其一般狀況,比較各組胎鼠體重、著床數(shù)、死胎及吸收胎的差異。6)分離血清檢測總膽固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白水平;7)留取孕鼠肝臟組織觀察稱重,蘇木精-伊紅染色法觀察其組織形態(tài)學(xué)的變化;8)酶聯(lián)免疫吸附法檢測炎性因子:腫瘤壞死因-α(TNF-α)、白介素-6(IL-6)的水平。結(jié)果:1)NF組、HF組和SHF組大鼠體重增加在喂養(yǎng)的第3周時明顯高于BC組,差異有統(tǒng)計學(xué)意義(P0.05);2)孕第18天OGTT結(jié)果顯示與BC組相比,HF組服糖15min后血糖吸收的峰值出現(xiàn)且明顯高于BC組和NF組(P0.05),SHF組服糖30min后出現(xiàn)峰值,較其它各組明顯延遲(P0.05);3)孕第20天,高劑量和超高劑量果糖孕鼠胰島素水平升高、胰島素敏感指數(shù)(ISI)及胰島素抵抗指數(shù)(HOMA-IR)降低,差異顯著(P0.05)。胰腺組織病理切片示HF組和SHF組胰島β細胞出現(xiàn)不同程度的肥大、增生。4)孕期結(jié)束,HF組死胎比例分別為9.1%,明顯高于BC組,SHF組吸收胎、死胎比例分別為10.0%和21.81%,明顯高于BC組且SHF組胎鼠體重明顯低于BC組(P0.05)。5)各果糖干預(yù)組血脂水平出現(xiàn)不同水平的升高,與BC組相比,HF和SHF組TC、TG水平明顯升高,SHF組LDL水平明顯升高,差異有統(tǒng)計學(xué)意義(P0.05)。肝臟病理切片觀察顯示,HF、SHF組有大小不等的脂質(zhì)空泡,出現(xiàn)輕度的脂肪沉積。6)HF和SHF組血清TNF-α和IL-6水平顯著升高(P0.05)。結(jié)論:1)孕期高劑量和超高劑量的果糖攝入可導(dǎo)致葡萄糖耐量受損和胰島素抵抗發(fā)生。2)孕期高劑量和超高劑量的果糖攝入可致不良妊娠結(jié)局。3)高劑量和超高劑量果糖攝入致孕期糖代謝異常與果糖攝入引起的炎癥因子水平升高有關(guān)。
[Abstract]:Objective: to observe the effect of fructose intake on glucose metabolism in pregnant rats by intragastric administration of fructose in different doses, and to observe the levels of blood glucose, insulin and embryonic development in pregnant rats, and to compare the effects of fructose intake with different doses on glucose metabolism in pregnant rats. To explore the mechanism of abnormal glucose metabolism in pregnant rats induced by high fructose intake. Methods: adult female and male SD rats were fed adaptively for one week, mating at 2:1. Vaginal smears were taken in the next morning. Under microscope, 48 pregnant rats were randomly divided into 4 groups. In the blank control group, (BC), normal dose fructose group, (NF), high dose fructose group, (HF), ultrahigh dose fructose group (SHF), were given distilled water, 2.0 g / kg + 5.0 g / kg and 8.0g/kg fructose 1ml, respectively. On the 18th day of pregnancy, oral glucose tolerance test (OGTT),) was performed on the 20th day of gestation. 1) daily food intake was recorded, weight was weighed weekly, and food intake and weight gain were compared. 2) OGTT examination was performed on the 18th day of pregnancy. To assess glucose tolerance; 3) to measure fasting insulin levels, calculate insulin sensitivity index and insulin resistance index, and evaluate islet function; 4) to observe histomorphologic changes of pancreatic tissue by hematoxylin-eosin staining; 5) to take fetal mice by laparotomy. Observe its general condition, compare the difference of body weight, implantation number, dead fetus and absorbent fetus in each group. 6) detect total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein in serum. 7) observe the weight of liver tissue of pregnant rats. The histomorphologic changes were observed by hematoxylin-eosin staining, and the levels of inflammatory cytokines, tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (Elisa). Results: 1) the weight gain of NF group, HF group and SHF group was significantly higher than that of BC group at the 3rd week of feeding. The results of OGTT on the 18th day of gestation showed that the peak value of glucose absorption in HF group was significantly higher than that in BC group and NF group (P0.05) on the 18th day of pregnancy compared with that in BC group (P0.05), which was significantly delayed than that in other groups (P0.05); (3) the peak value of glucose absorption in HF group was higher than that in BC group and NF group (P0.05). The insulin level increased and the insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) decreased in high dose and high dose fructose pregnant rats (P0.05). Pancreatic histopathological sections showed that islet 尾 cells were hypertrophy in different degrees in HF group and SHF group. 4) at the end of pregnancy, the proportion of dead fetus in HF group was 9.1 significantly higher than that in BC group, and that in SHF group was higher than that in BC group. The proportion of dead fetus was 10.0% and 21.81%, respectively, which was significantly higher than that in BC group, and the body weight of fetal rats in SHF group was significantly lower than that in BC group (P0.05). Compared with BC group, TC,TG level in HF and SHF group was significantly higher, LDL level in SHF group was significantly higher than that in BC group (P0.05). The difference was statistically significant (P0.05). Liver pathological examination showed that there were lipid vacuoles with slight fat deposition in HF,SHF group and serum TNF- 偽 and IL-6 levels in SHF group were significantly higher than those in HF group (P0.05). Conclusion: 1) High-dose and ultra-high fructose intake during pregnancy can lead to impaired glucose tolerance and insulin resistance. 2) High-dose and ultra-high fructose intake during pregnancy can result in adverse pregnancy outcome. Abnormal glucose metabolism during pregnancy induced by glucose intake was associated with increased levels of inflammatory factors induced by fructose intake.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R153.1
本文編號：2270415
[Abstract]:Objective: to observe the effect of fructose intake on glucose metabolism in pregnant rats by intragastric administration of fructose in different doses, and to observe the levels of blood glucose, insulin and embryonic development in pregnant rats, and to compare the effects of fructose intake with different doses on glucose metabolism in pregnant rats. To explore the mechanism of abnormal glucose metabolism in pregnant rats induced by high fructose intake. Methods: adult female and male SD rats were fed adaptively for one week, mating at 2:1. Vaginal smears were taken in the next morning. Under microscope, 48 pregnant rats were randomly divided into 4 groups. In the blank control group, (BC), normal dose fructose group, (NF), high dose fructose group, (HF), ultrahigh dose fructose group (SHF), were given distilled water, 2.0 g / kg + 5.0 g / kg and 8.0g/kg fructose 1ml, respectively. On the 18th day of pregnancy, oral glucose tolerance test (OGTT),) was performed on the 20th day of gestation. 1) daily food intake was recorded, weight was weighed weekly, and food intake and weight gain were compared. 2) OGTT examination was performed on the 18th day of pregnancy. To assess glucose tolerance; 3) to measure fasting insulin levels, calculate insulin sensitivity index and insulin resistance index, and evaluate islet function; 4) to observe histomorphologic changes of pancreatic tissue by hematoxylin-eosin staining; 5) to take fetal mice by laparotomy. Observe its general condition, compare the difference of body weight, implantation number, dead fetus and absorbent fetus in each group. 6) detect total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein in serum. 7) observe the weight of liver tissue of pregnant rats. The histomorphologic changes were observed by hematoxylin-eosin staining, and the levels of inflammatory cytokines, tumor necrosis factor- 偽 (TNF- 偽) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (Elisa). Results: 1) the weight gain of NF group, HF group and SHF group was significantly higher than that of BC group at the 3rd week of feeding. The results of OGTT on the 18th day of gestation showed that the peak value of glucose absorption in HF group was significantly higher than that in BC group and NF group (P0.05) on the 18th day of pregnancy compared with that in BC group (P0.05), which was significantly delayed than that in other groups (P0.05); (3) the peak value of glucose absorption in HF group was higher than that in BC group and NF group (P0.05). The insulin level increased and the insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) decreased in high dose and high dose fructose pregnant rats (P0.05). Pancreatic histopathological sections showed that islet 尾 cells were hypertrophy in different degrees in HF group and SHF group. 4) at the end of pregnancy, the proportion of dead fetus in HF group was 9.1 significantly higher than that in BC group, and that in SHF group was higher than that in BC group. The proportion of dead fetus was 10.0% and 21.81%, respectively, which was significantly higher than that in BC group, and the body weight of fetal rats in SHF group was significantly lower than that in BC group (P0.05). Compared with BC group, TC,TG level in HF and SHF group was significantly higher, LDL level in SHF group was significantly higher than that in BC group (P0.05). The difference was statistically significant (P0.05). Liver pathological examination showed that there were lipid vacuoles with slight fat deposition in HF,SHF group and serum TNF- 偽 and IL-6 levels in SHF group were significantly higher than those in HF group (P0.05). Conclusion: 1) High-dose and ultra-high fructose intake during pregnancy can lead to impaired glucose tolerance and insulin resistance. 2) High-dose and ultra-high fructose intake during pregnancy can result in adverse pregnancy outcome. Abnormal glucose metabolism during pregnancy induced by glucose intake was associated with increased levels of inflammatory factors induced by fructose intake.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R153.1
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