【摘要】：通過(guò)溶血試驗(yàn)和小鼠急性毒性實(shí)驗(yàn),評(píng)價(jià)市售水產(chǎn)品中創(chuàng)傷弧菌和標(biāo)準(zhǔn)創(chuàng)傷弧菌ATCC27562菌株毒性大小。從市售水產(chǎn)品中分離獲得的6株疑似創(chuàng)傷弧菌菌株,經(jīng)生化鑒定和溶細(xì)胞素基因擴(kuò)增、基因測(cè)序后證實(shí),除2號(hào)分離菌株基因測(cè)序未測(cè)出外,其它5株確定為創(chuàng)傷弧菌。創(chuàng)傷弧菌標(biāo)準(zhǔn)菌株ATCC27562菌株擴(kuò)增出400 bp左右的基因條帶,而6株分離株擴(kuò)增出222 bp左右的典型創(chuàng)傷弧菌溶細(xì)胞素基因條帶。在溶血試驗(yàn)中,創(chuàng)傷弧菌ATCC27562菌株的溶血圈與菌落直徑之比達(dá)到3.0,1~6號(hào)分離株分別為2.3,2.0,2.8,2.0,2.5和2.1。在小鼠急性毒性實(shí)驗(yàn)中,創(chuàng)傷弧菌ATCC27562菌株的半致死量(LD_(50))是3.776×10~7CFU/m L,而3號(hào)分離株的LD_(50)為2.218×10~8CFU/m L。創(chuàng)傷弧菌ATCC27562菌株為較強(qiáng)毒株,而3號(hào)分離株為弱毒株。
[Abstract]:The toxicity of Vibrio vulnificus and standard Vibrio vulnificus (ATCC27562) in aquatic products was evaluated by hemolysis test and acute toxicity test in mice. Six vibrio vulnificus strains isolated from aquatic products on the market were identified as vibrio vulnificus by biochemical identification and amplification of cytolytic gene. After gene sequencing, it was confirmed that the other 5 strains were confirmed as Vibrio vulnificus, except that the gene sequence of strain 2 was not detected. About 400 bp gene bands were amplified from the standard strain of Vibrio vulnificus (ATCC27562), and about 222 bp of typical Vibrio vulnificus lysogenin gene bands were amplified from 6 strains of Vibrio vulnificus. In the hemolytic test, the ratio of hemolytic circle to colony diameter of Vibrio vulnificus ATCC27562 strain was 2. 3 / 2. 0 ~ 2. 8 / 2. 0 and 2. 1 respectively. The semi-lethal dose (LD_ _ (50) of Vibrio vulnificus ATCC27562 strain was 3.776 脳 10~7CFU/m / L and the LD_ _ (50) of strain 3 was 2.218 脳 10~8CFU/m _ L in acute toxicity test in mice. Vibrio vulnificus ATCC27562 was a strong strain, while strain 3 was a attenuated strain.
【作者單位】： 浙江工商大學(xué)食品與生物工程學(xué)院浙江省食品安全重點(diǎn)實(shí)驗(yàn)室;渤海大學(xué)化學(xué)化工與食品安全學(xué)院遼寧省食品安全重點(diǎn)實(shí)驗(yàn)室;杭州奧泰生物技術(shù)有限公司;
【基金】：“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2012BAD29B06)
【分類號(hào)】：R155.5

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