發(fā)布時(shí)間：2018-08-14 19:05

【摘要】：目的：研究PAHs暴露致工人早期外周血淋巴細(xì)胞DNA損傷情況，并進(jìn)一步探討尿多環(huán)芳烴代謝物、血漿BPDE-白蛋白加合物與DNA損傷之間的關(guān)系。 方法：選取湖北省武漢市某焦化廠1167名男性工人作為研究對(duì)象，選取辦公室、輔助車(chē)間、爐底和爐側(cè)、爐頂?shù)却硇怨ぷ鲘徫贿M(jìn)行工作場(chǎng)所PAHs濃度的檢測(cè)；對(duì)所有工人都進(jìn)行職業(yè)健康體檢；同時(shí)采用調(diào)查表的形式收集每個(gè)工人的人口學(xué)信息、吸煙飲酒史、既往史、家族遺傳史以及職業(yè)暴露史；采集每個(gè)工人尿液，使用氣相色譜-質(zhì)譜聯(lián)用儀（GC-MS）檢測(cè)尿液中10種多環(huán)芳烴代謝物的含量，，評(píng)價(jià)工人職業(yè)性PAHs內(nèi)暴露水平；酶聯(lián)免疫吸附試驗(yàn)（ELISA）檢測(cè)血漿BPDE-白蛋白濃度，評(píng)價(jià)PAHs暴露有效劑量水平；彗星試驗(yàn)檢測(cè)外周血淋巴細(xì)胞DNA損傷程度，采用Olive尾矩（Olive tail moment, OTM）指標(biāo)來(lái)評(píng)價(jià)PAHs致機(jī)體早期健康損害情況。將各代謝物四分位以分析其與DNA損傷之間劑量-效應(yīng)關(guān)系。 結(jié)果：除四羥基菲外其余九種尿多環(huán)芳烴代謝物、BPDE-白蛋白加合物及OTM均呈現(xiàn)辦公室組＜輔助崗位組＜爐底和爐側(cè)組＜爐頂組的趨勢(shì)，且四組間差異具有統(tǒng)計(jì)學(xué)意義；Pearson相關(guān)分析發(fā)現(xiàn)1-羥基萘與所有OH-PAHs相關(guān)性最好，而1-羥基芘與BPDE-白蛋白相關(guān)性最好，除9-羥基芴、3-，4-羥基菲外其余七種尿OH-PAHs、BPDE-白蛋白加合物均與OTM呈正相關(guān)；以正相關(guān)的OH-PAHs濃度及BPDE-白蛋白加合物濃度四分位分組，都與OTM呈劑量-效應(yīng)關(guān)系；1-羥基萘與BPDE-白蛋白加合物聯(lián)合分析表明，與1-羥基萘第一組和BPDE-白蛋白加合物濃度最低組相比，1-羥基萘第四組和BPDE-白蛋白加合物濃度最高組DNA損傷水平升高有顯著性（p＜0.001），而且與其他組相比升高的水平最多。 結(jié)論：外周血淋巴細(xì)胞DNA損傷隨職業(yè)性PAHs暴露水平增高而增加；尿7種OH-PAHs、血漿BPDE-白蛋白加合物與DNA損傷呈顯著劑量-效應(yīng)關(guān)系；1-羥基萘與BPDE-白蛋白加合物對(duì)PAHs暴露所造成的焦?fàn)t工外周血淋巴細(xì)胞DNA損傷具有聯(lián)合作用。
[Abstract]:Objective: to study the DNA damage in peripheral blood lymphocytes of workers exposed to PAHs in early stage, and to explore the relationship between urinary polycyclic aromatic hydrocarbons metabolites, plasma BPDE-albumin adducts and DNA damage. Methods: 1167 male workers from a coking plant in Wuhan, Hubei Province were selected as the research objects. The PAHs concentration in the workplace was measured in representative work positions such as office, auxiliary workshop, furnace bottom, furnace side, furnace top and so on. All workers were examined for occupational health; demographics of each worker were collected in the form of questionnaires; smoking and drinking history, past history, family genetic history and occupational exposure history were collected; urine samples were collected from each worker. Ten polycyclic aromatic hydrocarbons (PAHs) metabolites in urine were detected by gas chromatography-mass spectrometry (GC-MS) to evaluate the exposure level of occupational PAHs in workers, the plasma BPDE- albumin concentration was detected by Elisa (ELISA), and the effective dose level of PAHs exposure was evaluated. Comet assay was used to detect the degree of DNA damage in peripheral blood lymphocytes and Olive tail moment (Olive tail moment, OTM) index was used to evaluate the early health damage caused by PAHs. Four quartiles of metabolites were used to analyze the dose-effect relationship between the metabolites and DNA damage. Results: nine urinary polycyclic aromatic hydrocarbons metabolites (BPDE- albumin adducts and OTM) except tetrahydroxy phenanthrene showed the trend of office group < auxiliary post group < furnace bottom group and furnace side group < furnace top group, and the differences among the four groups were statistically significant. Pearson correlation analysis showed that 1-hydroxynaphthalene had the best correlation with all OH-PAHs, while 1-hydroxypyrene had the best correlation with BPDE-albumin. The other seven urinary OH-PAHsBPDE-albumin adducts were positively correlated with OTM except 9-hydroxyfluorene 3-4-hydroxyphenanthrene. According to the positive correlation of OH-PAHs concentration and the concentration of BPDE- albumin adducts, there was a dose-effect relationship between BPDE-albumin adducts and 1-hydroxylnaphthalene and BPDE-albumin adducts. Compared with the first group of 1-hydroxynaphthalene and the lowest concentration group of BPDE-albumin adducts, the level of DNA damage in the fourth group and the highest concentration group was significantly increased (p < 0.001), and the highest level was found in the other groups. Conclusion: the DNA damage of peripheral blood lymphocytes increased with the increase of occupational PAHs exposure, and there was a significant dose-effect relationship between urinary seven OH-PAHs, plasma BPDE-albumin adducts and DNA damage. 1-hydroxynaphthalene and BPDE-albumin adducts have a combined effect on DNA damage in peripheral blood lymphocytes of coke oven workers caused by PAHs exposure.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R131

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