【摘要】：為探究從壇紫菜中提取的類(lèi)菌孢素氨基酸(MAAs)化合物對(duì)紫外線(xiàn)誘導(dǎo)產(chǎn)生的小鼠皮膚光老化作用的保護(hù)機(jī)制,實(shí)驗(yàn)采用乙醇熱水提取法,提取壇紫菜中的MAAs,對(duì)其化合物的基本成分組成進(jìn)行鑒定;通過(guò)觀測(cè)小鼠造模部位皮膚的損傷情況,結(jié)合皮膚病理切片來(lái)評(píng)價(jià)壇紫菜MAAs對(duì)光老化小鼠的保護(hù)作用,并通過(guò)測(cè)定小鼠組織中超氧化物歧化酶(T-SOD)、谷胱甘肽過(guò)氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量和皮膚組織中羥脯氨酸(Hyp)的含量,分析MAAs化合物對(duì)光老化皮膚的保護(hù)機(jī)制。結(jié)果顯示,從壇紫菜中提取的類(lèi)菌孢素氨基酸類(lèi)化合物主要組分為Porphyra-334和Shinorine;紫菜MAAs可有效改善因紫外線(xiàn)誘導(dǎo)而產(chǎn)生的皮膚光老化的發(fā)生;與模型組小鼠相比,外涂給藥組小鼠皮膚組織中SOD、GSH-Px的活性及Hyp的含量顯著性提高,MDA的含量顯著降低;而灌胃給藥組與空白對(duì)照組小鼠相比體內(nèi)抗氧化酶活性作用水平變化不明顯。研究表明,紫菜MAAs對(duì)紫外線(xiàn)誘導(dǎo)的皮膚光老化具有保護(hù)作用。
[Abstract]:In order to explore the protective mechanism of (MAAs) compounds extracted from porphyra chinensis on ultraviolet induced photoaging of mouse skin, ethanol hot water extraction was used in the experiment. The basic composition of the compound was identified by extracting MAAs.The protective effect of porphyra chinensis MAAs on photoaging mice was evaluated by observing the skin damage at the model site and combining the pathological sections of the skin. The activities of superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and hydroxyproline (Hyp) in skin were determined to analyze the protective mechanism of MAAs compounds on photoaging skin. The results showed that the main components of the amino acids extracted from porphyra chinensis were Porphyra-334 and Shinorine, and MAAs could effectively improve the occurrence of skin photoaging induced by ultraviolet radiation. The activity of SOD- GSH-Px and the content of Hyp in skin tissue of mice treated with the drug were significantly decreased, but the level of antioxidant enzyme activity in mice treated with oral administration had no obvious change compared with the control group. The results showed that MAAs had protective effect on UV-induced photoaging of skin.
【作者單位】： 中國(guó)海洋大學(xué)食品科學(xué)與工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31272705)~~
【分類(lèi)號(hào)】：R151;TS974.1

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1 吳寧霜;朱玉霞;程虹;;皮膚光老化及其防治[J];科技信息(學(xué)術(shù)研究);2008年18期

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本文編號(hào)：2180169