【摘要】：目的探討980 nm紅外連續(xù)激光對(duì)金屬醫(yī)療器械表面高效滅菌的功率和時(shí)間閾值關(guān)系。方法采用人工污染實(shí)驗(yàn)法,以無(wú)菌鋼制手術(shù)刀為載體,用金黃色葡萄球菌和大腸桿菌分別進(jìn)行污染,經(jīng)不同功率、對(duì)應(yīng)不同輻照時(shí)間的激光消毒滅菌后用細(xì)菌培養(yǎng)基清洗刀片采樣,采用增菌培養(yǎng)來(lái)檢測(cè)紅外激光對(duì)大腸桿菌和金黃色葡萄球菌的滅活情況。結(jié)果 980 nm激光在5.24 W時(shí)輻照29 s,6.87 W時(shí)輻照24 s,8.33 W時(shí)輻照14 s,10.11 W時(shí)輻照14 s都能徹底殺死金黃色葡萄球菌;980 nm激光在5.24 W時(shí)輻照22 s,6.87 W時(shí)輻照12 s,8.33 W時(shí)輻照11 s,10.11 W時(shí)輻照9 s都能徹底殺死大腸桿菌。大腸桿菌比金黃色葡萄球菌更易滅活。依據(jù)實(shí)驗(yàn)數(shù)據(jù)擬合,在同一激光功率下,兩種細(xì)菌存活率與輻照時(shí)間的函數(shù)符合一階反應(yīng)動(dòng)力學(xué)形式。結(jié)論 980 nm紅外激光能夠?qū)崿F(xiàn)金屬醫(yī)療器械表面的快速消毒滅菌,并且功率越高則時(shí)間越短、效果越好。
[Abstract]:Objective to investigate the relationship between power and time threshold of 980 nm infrared continuous laser sterilization on the surface of metal medical instruments. Methods the method of artificial pollution experiment was used, the sterile steel scalpel was used as the carrier, Staphylococcus aureus and Escherichia coli were used to pollute the scalpel with different power. After sterilizing and sterilizing with laser at different irradiation time, the blade samples were washed with bacterial culture medium, and the inactivation of Escherichia coli and Staphylococcus aureus by infrared laser was detected by bacteria culture. Results 980 nm laser irradiation at 5.24 W, 29 s-1, 6.87 W, 24 s-1, 8.33 W, 14 sL, 10.11 W for 14 s, can completely kill Staphylococcus aureus at 980 nm laser at 5.24 W, 22 sL, 6.87 W, 12 sL, 8.33 W, 11 s10.11, respectively. When exposed to W for 9 s, Escherichia coli can be killed completely. E. coli is more inactivated than Staphylococcus aureus. According to the experimental data fitting, under the same laser power, the function of the survival rate and irradiation time of the two bacteria accords with the first-order reaction kinetics. Conclusion 980 nm infrared laser can quickly sterilize the surface of metal medical instruments, and the higher the power is, the shorter the time is and the better the effect is.
【作者單位】： 黃山學(xué)院信息工程學(xué)院;工程物理研究院流體物理研究所;第三軍醫(yī)大學(xué)西南醫(yī)院神經(jīng)外科;西南醫(yī)科大學(xué)第一附屬醫(yī)院神經(jīng)外科;
【基金】：中國(guó)博士后科學(xué)基金(2014M552542XB,2015T80989) 國(guó)家自然科學(xué)基金面上項(xiàng)目(81402067) 黃山學(xué)院人才啟動(dòng)項(xiàng)目(2016xkjq001) 中國(guó)工程物理研究院基金(JM20150);中國(guó)工程物理研究院物理與生物醫(yī)學(xué)交叉實(shí)驗(yàn)室孵化基金(JCFH)~~
【分類號(hào)】：R187

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