本文選題：鄰苯二甲酸二(-乙基)己酯 + 雙酚A��； 參考：《環(huán)境與健康雜志》2015年03期

【摘要】：目的研究環(huán)境內(nèi)分泌污染物鄰苯二甲酸二(2-乙基)己酯(DEHP)和雙酚A(BPA)對(duì)原代大鼠睪丸間質(zhì)細(xì)胞活性的影響。方法采用酶消化與貼壁法相結(jié)合提取大鼠睪丸間質(zhì)細(xì)胞,分別暴露于終濃度0(對(duì)照)、1、10、100、1 000μmol/L的DEHP和(或)BPA溶液24 h。采用CCK8法檢測(cè)細(xì)胞活性。結(jié)果與對(duì)照組比較,100、1 000μmol/L的DEHP暴露組和10、100、1 000μmol/L BPA暴露組及各濃度DEHP+BPA聯(lián)合暴露組大鼠睪丸間質(zhì)細(xì)胞的抑制率均較高,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);且隨著DEHP和(或)BPA暴露濃度的升高,大鼠睪丸間質(zhì)細(xì)胞的抑制率均呈上升趨勢(shì)。與相同濃度DEHP+BPA聯(lián)合暴露組比較,1、1 000μmol/L的DEHP暴露組大鼠睪丸間質(zhì)細(xì)胞的抑制率較低,1 000μmol/L的BPA暴露組大鼠睪丸間質(zhì)細(xì)胞的抑制率較高,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。1μmol/L的DEHP與BPA聯(lián)合暴露對(duì)細(xì)胞活性抑制表現(xiàn)為協(xié)同作用,1 000μmol/L的DEHP與BPA聯(lián)合暴露表現(xiàn)為拮抗作用,10和100μmol/L的DEHP與BPA聯(lián)合暴露對(duì)抑制細(xì)胞活性未表現(xiàn)出交互作用。結(jié)論 DEHP和BPA均能顯著影響睪丸間質(zhì)細(xì)胞活性,且以BPA作用更加明顯;低劑量(1μmol/L)的DEHP和BPA聯(lián)合作用為協(xié)同效應(yīng),高劑量(1 000μmol/L)的DEHP和BPA聯(lián)合作用為拮抗效應(yīng)。
[Abstract]:Objective to study the effects of environmental endocrine pollutants (DEHP) and bisphenol A (BPA) on the activity of primary rat testicular stromal cells. Methods Leydig cells of rat testis were extracted by enzyme digestion and adherent method. The cells were exposed to DEHP and / or BPA solution of 100 渭 mol / L 0 (control) for 24 h. The cell activity was detected by CCK 8 method. Results compared with the control group, the inhibition rate of Leydig cells was higher in DEHP exposure group (100 渭 mol / L), BPA exposure group (100 渭 mol / L), and DEHP BPA combined exposure group (P0.05), and with the increase of DEHP and (or) BPA exposure concentration, the inhibition rate of Leydig cells in testis was significantly higher than that in control group (P0.05), and with the increase of DEHP and (or) BPA exposure concentration, the inhibition rate of Leydig cells was significantly higher than that of control group (P0.05). The inhibitory rate of Leydig cells in rat testis showed an increasing trend. Compared with the same concentration DEHP BPA combined exposure group, the inhibition rate of testicular interstitial cells in DEHP exposure group was lower than that in BPA exposure group at 1 000 渭 mol / L, and the inhibition rate of testicular interstitial cells in DEHP exposure group was higher than that in BPA exposure group at 1 000 渭 mol / L. The difference was statistically significant (P0.05). 1 渭 mol / L combined exposure of DEHP and BPA showed synergistic effect on cell activity inhibition. The combined exposure of DEHP and BPA to 1 000 渭 mol / L showed antagonistic effect and 100 渭 mol / L DEHP and BPA combined exposure showed no interaction on the inhibition of cell activity. Conclusion both DEHP and BPA can significantly affect the activity of Leydig cells, especially BPA, and the combination of DEHP and BPA at a low dose (1 渭 mol / L) is a synergistic effect, and the combination of DEHP and BPA at a high dose (1 000 渭 mol / L) is an antagonistic effect.
【作者單位】： 沈陽醫(yī)學(xué)院公共衛(wèi)生學(xué)院;
【基金】：國家自然科學(xué)基金(81373029) 遼寧省教育廳科學(xué)技術(shù)研究項(xiàng)目(L2012377) 沈陽市科技計(jì)劃項(xiàng)目(F13-302-1-00) 遼寧省高等學(xué)校杰出青年學(xué)者成長(zhǎng)計(jì)劃(LJQ2014114) 沈陽醫(yī)學(xué)院科技基金(20121005)
【分類號(hào)】：R114

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本文編號(hào)：2091927