本文選題：伏馬毒素 + 氯霉素　； 參考：《天津科技大學(xué)》2012年碩士論文

【摘要】：論文采用一種免疫親和凝膠柱對食品中伏馬毒素B1(FB1)和氯霉素(CAP)殘留的檢測進行了系統(tǒng)研究,用溴化氫活化的瓊脂糖凝膠偶聯(lián)伏馬毒素抗體或氯霉素抗體制備其分別的抗體膠；偶聯(lián)辣根過氧化氫酶(HRP)抗體制備其HRP膠；將溴化氫活化的瓊脂糖凝膠封閉制備封閉膠。封閉膠與抗體膠混合制備成檢測層,與HRP膠混合制備成對照層,裝入檢測柱,并對影響實驗的各個因素(離子強度,pH值等)進行了優(yōu)化,建立了一種省時、靈敏度高、可視化的檢測方法,可以定性半定量的檢測食品中的伏馬毒素和氯霉素。 對于伏馬毒素B1免疫親和凝膠檢測柱：封閉膠與抗體膠以1：10比例混合可以達到良好的視覺顯色結(jié)果,封閉膠與抗體膠1：90混合制備其對照層,其FB1-HRP以1：8000稀釋用于凝膠檢測柱的檢測,建立伏馬毒素免疫親和凝膠檢測柱的最低限量檢測限(LOD)為40μg/L。對啤酒、白酒、玉米粉、谷物牛奶中伏馬毒素殘留進行了檢測：啤酒和白酒直接稀釋5倍；玉米粉加入PBS提取離心后稀釋5倍；谷物牛奶離心取上層溶液稀釋10倍。 對于氯霉素免疫親和凝膠檢測柱：封閉膠與抗體膠以1：20比例混合是可以達到良好的視覺顯色結(jié)果,封閉膠與抗體膠1：100混合制備其對照層,其CAP-HRP以1：20萬稀釋用于凝膠檢測柱的檢測,建立氯霉素免疫親和凝膠檢測柱的LOD為1μg/L。對牛奶、乳粉、魚塘水、蜂蜜中氯霉素的殘留進行了檢測；牛奶和魚塘水可以直接進行檢測；乳粉5倍加入PBS溶解；蜂蜜5倍稀釋。 該免疫親和凝膠柱檢測食品樣品不需要任何有機溶劑和復(fù)雜的前處理,及大型儀器的輔助,添加3個不同濃度的伏馬毒素(氯霉素)標(biāo)品,用ELISA方法進行了驗證,檢測結(jié)果一致,表明試驗所建立的方法具有很好的準(zhǔn)確性,滿足快速檢測的要求。
[Abstract]:The detection of FB1 and CAP residues in food was systematically studied by an immuno-affinity gel column. The agarose gel coupled with fumaroxin antibody or chloramphenicol antibody was used to prepare its respective antibody glue. Horseradish catalase (HRP) antibody was coupled to prepare the HRP gel, and the agarose gel activated by hydrogen bromide was blocked to prepare the sealant. The sealant and antibody gel were mixed to prepare the detection layer, and the HRP gel was mixed to form the contrast layer. The test column was mounted. The factors affecting the experiment (ionic strength, pH value, etc.) were optimized, and a time-saving and high sensitivity was established. Visual detection method can be used to detect fumaroxin and chloramphenicol in food qualitatively and quantitatively. For Fumatoxin B1 immuno-affinity gel column: the mixture of sealant and antibody glue at 1:10 can achieve good visual color results, and the control layer was prepared by mixing sealant and antibody glue 1:90. The FB1-HRP was diluted with 1: 8000 to detect the gel column. The minimum detection limit (LOD) of the FB1-HRP gel column was 40 渭 g 路L ~ (-1) 路L ~ (-1). The residue of fumarin in beer, liquor, cornmeal and cereal milk was determined: beer and liquor were diluted 5 times directly, corn flour was diluted by 5 times after PBS extraction, and the upper layer solution of cereal milk was diluted by 10 times. For chloramphenicol immunoaffinity gel detection column: the mixture of sealant and antibody glue at 1:20 can achieve good visual color result, and the control layer was prepared by mixing the sealant and antibody glue 1: 100. The CAP-HRP was diluted at 1: 200 000 to detect the gel column. The LOD of the established gel column was 1 渭 g / L. The residues of chloramphenicol in milk, milk powder, fish pond water and honey were detected; milk and fish pond water could be directly detected; milk powder was dissolved by PBS; honey was diluted by 5 times. The immunoaffinity gel column was used to detect food samples without any organic solvent, complex pretreatment, and the aid of large scale instrument. Three different concentrations of chloramphenicol (chloramphenicol) were added to food samples, and the results were verified by Elisa. The results show that the proposed method has good accuracy and meets the requirement of rapid detection.
【學(xué)位授予單位】：天津科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R155.5

【參考文獻】

相關(guān)期刊論文 前10條

1 徐修遠,滑靜,胡建民,楊柳,王建立;氯霉素在動物體內(nèi)殘留問題的研究進展[J];中國畜牧獸醫(yī);2004年10期

2 劉江;伏馬菌素──食品衛(wèi)生及真菌毒素研究中的新熱點[J];國外醫(yī)學(xué).衛(wèi)生學(xué)分冊;1995年06期

3 李春生;;免疫檢測技術(shù)在農(nóng)畜產(chǎn)品安全檢測中的應(yīng)用[J];河北省科學(xué)院學(xué)報;2009年01期

4 盧利軍;于徊萍;周曉;李愛軍;郭錦泉;;固相萃取-流動注射-化學(xué)發(fā)光法測定雞飼料和雞肉中的氯霉素[J];化學(xué)試劑;2007年08期

5 王自良,趙坤,張改平;氯霉素的毒性及其在動物性食品中的殘留與檢測[J];河南科技學(xué)院學(xué)報(自然科學(xué)版);2005年02期

6 沈美芳,趙文亞,費志良,吳光紅,耿雪冰;用酶聯(lián)免疫法測定水產(chǎn)品中氯霉素的殘留量[J];南京師范大學(xué)學(xué)報(工程技術(shù)版);2003年02期

7 陳美蓮;張輝;;乳與乳制品中氯霉素殘留量的檢測方法[J];乳業(yè)科學(xué)與技術(shù);2006年01期

8 郝俊虎,王仙琴,閆永利,李萍,馬昕;氯霉素殘留對出口畜產(chǎn)品的危害[J];飼料研究;2005年05期

9 宮向紅;徐英江;張秀珍;劉麗娟;;水產(chǎn)品中氯霉素殘留量氣相色譜檢測方法的探討[J];食品科學(xué);2006年07期

10 秦紅;韓劍眾;;動物性食品氯霉素殘留檢測技術(shù)研究進展[J];食品研究與開發(fā);2006年10期

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