本文選題：50Hz磁場 + 大鼠��； 參考：《第三軍醫(yī)大學》2012年碩士論文

【摘要】：目的隨著經濟的發(fā)展和信息時代的來臨，各種類型的電子產品及電子設備在家庭和工作環(huán)境中的大量使用，使人們暴露于50Hz電磁場的機會與日俱增。電器產品給人們帶來了諸多方便的同時，其電磁輻射等負面效應逐步引起人們的廣泛關注。本研究以臨床常用血液檢驗和病理檢測技術為手段，系統(tǒng)觀察50Hz磁場三種等比劑量暴露對大鼠血液學和生物化學指標和相關臟器組織形態(tài)學的影響規(guī)律，旨在進一步研究觀察50Hz磁場的生物學效應，，嘗試尋找相關血清標志物，為接觸者健康監(jiān)護和衛(wèi)生事業(yè)管理（包括特殊健康檢查項目的確定、健康監(jiān)護檔案管理、健康狀況分析、評價預防措施效果、修訂衛(wèi)生標準等）提供線索和依據(jù)。 方法將SD雄性大鼠隨機分為1個對照組和3個輻照組，分別全身暴露于0μT（對照組）、20μT、100μT及500μT50Hz磁場中，每天2小時，連續(xù)4周，每周測其體重變化，在暴露結束后采集外周血和心、肝、腎、睪丸等組織，檢測血清肝功能、腎功能、血脂、心肌酶譜、睪酮水平和外周血細胞數(shù)量等指標，稱重并計算心、肝、腎、睪丸的臟器系數(shù)，光鏡病理學分析各臟器組織形態(tài)結構的變化。 結果50Hz磁場暴露期間各組大鼠體重無明顯差異。20μT和100μT劑量暴露對血清肝功能、腎功能、血脂、心肌酶譜、睪酮水平、各組織臟器系數(shù)及外周血細胞數(shù)量均無明顯影響。500μT劑量暴露后大鼠血清肌酐水平較對照組顯著升高（P0.05），總膽固醇水平較對照組明顯降低（P0.05），血清睪酮水平較對照組顯著升高（P0.05），心、肝、腎、睪丸組織臟器系數(shù)均無明顯差異（P0.05），外周血白細胞數(shù)量較對照組顯著增加（P0.05）。進一步相關分析發(fā)現(xiàn)，血清肌酐、總膽固醇、睪酮、外周血白細胞數(shù)量與50Hz磁場暴露水平之間存在明顯的相關關系（p均0.05）；血清葡萄糖和肌酸激酶水平與50Hz磁場暴露水平之間均存在正相關關系趨勢（p均0.05）。HE染色發(fā)現(xiàn)，大鼠心肌間質血管擴張充血；肝中央靜脈及肝竇擴張充血，周圍肝細胞水腫；腎小管上皮細胞濁腫，間質血管擴張充血；睪丸間質組織可見局灶性充血水腫現(xiàn)象；以上各組織形態(tài)結構的變化隨著暴露劑量的增加，充血水腫越明顯，呈一定的劑量-效應關系。 結論在本實驗條件下，50Hz磁場暴露對雄性大鼠生長發(fā)育無明顯影響，可引起臟器組織呈現(xiàn)一定的血管通透性改變；高劑量（500μT）暴露可引起雄性大鼠腎臟功能、睪丸功能、血脂代謝以及外周血白細胞數(shù)量的變化；血清肌酐、總膽固醇、睪酮、外周血白細胞數(shù)量可能是50Hz磁場暴露生物學效應潛在的血清標志物，需要進一步研究證實。
[Abstract]:Objective with the development of economy and the advent of the information age, various types of electronic products and electronic devices are widely used in the home and work environment, which make people exposed to 50Hz electromagnetic field day by day. Electrical products bring people a lot of convenience, at the same time, its electromagnetic radiation and other negative effects gradually aroused widespread concern. In this study, the effects of 50 Hz magnetic field on hematological and biochemical indexes and histomorphology of related organs in rats were systematically observed by means of blood test and pathological examination. The aim of this study was to investigate the biological effects of 50Hz magnetic field and to search for serum markers for health monitoring and health management (including the determination of special health examination items, health monitoring file management, health status analysis). To evaluate the effect of preventive measures, to revise the health standards, etc.) to provide clues and evidence. Methods Sprague-Dawley male rats were randomly divided into one control group and three irradiation groups. The rats were exposed to 0 渭 T 100 渭 T 100 渭 T and 500 渭 T 50Hz magnetic field for 2 hours a day for 4 weeks. The body weight was measured weekly. The peripheral blood and heart were collected after exposure. Liver, kidney, testis, serum liver function, renal function, blood lipid, myocardial enzyme spectrum, testosterone level and the number of peripheral blood cells were measured, and the organ coefficients of heart, liver, kidney and testis were calculated. Histological changes of various organs were analyzed by light microscopy. Results during 50 Hz magnetic field exposure, there was no significant difference in body weight between each group. The dose of 20 渭 T and 100 渭 T had no effect on serum liver function, renal function, blood lipid, myocardial enzyme spectrum and testosterone level. The serum creatinine level, total cholesterol level and serum testosterone level were significantly higher than those in the control group (P0.05), and the serum testosterone level was significantly higher than that of the control group (P0.05), and the serum testosterone level was significantly higher than that of the control group (P0.05), and the total cholesterol level was significantly lower than that of the control group (P0.05), and the serum testosterone level was significantly higher than the control group (P0.05). There was no significant difference in organ coefficient among liver, kidney and testis (P0.05), and the number of peripheral blood leukocytes was significantly increased compared with control group (P0.05). Further correlation analysis showed that there was a significant correlation between serum creatinine, total cholesterol, testosterone, the number of peripheral white blood cells and 50 Hz magnetic field exposure (p0.05). There was a positive correlation between serum glucose and creatine kinase levels and 50 Hz magnetic field exposure (p0.05) .HE staining showed that the myocardial interstitial vasodilation of rats was congested, the central hepatic vein and sinusoidal dilatation hyperemia, and the peripheral hepatocyte edema. Renal tubule epithelial cell turbid, interstitial vascular dilatation and hyperemia; testicular interstitial tissue showed focal hyperemia and edema. The changes of morphology and structure of the above tissues increased with the increase of exposure dose, the hyperemia and edema became more obvious. There is a dose-effect relationship. Conclusion under this condition, 50 Hz magnetic field exposure has no obvious effect on the growth and development of male rats, and can cause certain vascular permeability changes in visceral tissues, and high dose (500 渭 T) exposure can induce renal function and testicular function in male rats. Serum creatinine, total cholesterol, testosterone and the number of peripheral white blood leukocytes may be potential serum markers for biological effects exposed to 50 Hz magnetic field.
【學位授予單位】：第三軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R114

【參考文獻】

相關期刊論文 前10條

1 林孟端;陳昱;陳華;王修景;;不同強度的低功率微波輻射對雄性小鼠生殖系統(tǒng)的影響[J];癌變.畸變.突變;2007年06期

2 卑偉慧;曹毅;;電磁輻射的生物學效應[J];輻射防護通訊;2007年03期

3 張巍;康鍶鵬;陳清瑞;蔡宗達;王碧霞;王瑋;;巴戟天對微波損傷的雄鼠睪丸生精功能的影響[J];解剖學研究;2010年05期

4 舒如明;谷翊群;;電磁輻射對雄性生殖的影響[J];國際生殖健康/計劃生育雜志;2009年06期

5 鄧樺,王德文,彭瑞云,王水明,陳建魁,張颯,董波,王曉民;非電離輻射對大鼠心肌細胞的損傷及其機制[J];環(huán)境與健康雜志;2004年03期

6 陳浩宇,王水明;電磁輻射對男性生殖系統(tǒng)的影響[J];軍事醫(yī)學科學院院刊;2005年02期

7 楊進清;王虛步;劉勇;;電磁輻照致大鼠睪丸間質細胞超微結構的變化[J];檢驗醫(yī)學與臨床;2010年10期

8 王水明;彭瑞云;高亞兵;馬俊杰;陳浩宇;周紅梅;胡文華;張德添;王德文;;高功率微波輻射所致大鼠睪丸損傷的病理學研究[J];中華男科學雜志;2006年06期

9 閆素文;張寧;湯潔;魯海鷗;王喜良;;低劑量微波輻射對男性生殖力的影響[J];中華男科學雜志;2007年04期

10 康寧;商學軍;黃宇烽;;手機輻射對雄性生殖的影響[J];中華男科學雜志;2010年11期

本文編號：2072593