本文選題：納米氧化銅 + 家兔��； 參考：《環(huán)境與健康雜志》2017年07期

【摘要】：目的觀察納米氧化銅(Cu O)對家兔急性眼刺激性作用以及及時沖洗措施對于緩解急性刺激癥狀的有效性。方法40、80和200 nm近球形納米Cu O顆粒用于試驗(yàn)。納米Cu O先以0.5%羥丙基甲基纖維素(HPMC)分散后以生理鹽水稀釋配制成染毒分散懸液。新西蘭家兔用于試驗(yàn)。試驗(yàn)設(shè)40、80和200 nm納米Cu O組(分別按照10、40和160 mg/L劑量分組)和0.5%HPMC組(分散劑對照),每組3只動物。試驗(yàn)按照染毒不沖洗和染毒后4 s沖洗兩種方案進(jìn)行。不沖洗試驗(yàn):將納米Cu O測試液0.1 ml分別滴入到3只家兔左眼結(jié)膜囊中,并使眼睛被動閉合1 s,右眼不處理作自身對照;4 s沖洗試驗(yàn):家兔染毒后第4秒,用無菌生理鹽水徹底沖洗眼部30 s。并于染毒后1、24、48、72 h、4和7 d對動物眼睛進(jìn)行檢查和評分。結(jié)果 40 nm粒徑三個劑量組和80 nm粒徑40和160 mg/L劑量組動物左眼于染毒后(不沖洗)1 h出現(xiàn)眼結(jié)膜明顯充血,至24 h出現(xiàn)進(jìn)行性加重。80 nm和40 nm納米Cu O染毒組動物分別于染毒后48和72 h眼結(jié)膜充血時癥狀明顯緩解,至觀察第4天,所有動物充血癥狀消散。上述動物角膜和虹膜未觀察到損傷改變。200 nm粒徑納米Cu O所有劑量組動物均未觀察到眼部刺激癥狀。根據(jù)急性眼刺激性試驗(yàn)結(jié)果顯示,40 nm和80 nm粒徑納米Cu O眼刺激性反應(yīng)分級為可逆性眼損傷。在4 s沖洗試驗(yàn)中,40、80和200 nm組所有動物均未出現(xiàn)明顯眼部刺激癥狀。試驗(yàn)中0.5%HPMC對所有動物不具有眼刺激性。結(jié)論小粒經(jīng)的40和80 nm納米Cu O具有結(jié)膜損傷眼刺激性。及時(4 s)采取眼部沖洗措施能有效降低納米Cu O接觸引起的急性眼刺激癥狀。
[Abstract]:Objective to observe the effect of nano-cupric oxide (CuO) on acute eye irritation in rabbits and the effectiveness of timely washing measures in relieving acute irritation symptoms. Methods 40,80 nm and 200 nm spherical nano-CuO particles were used in the experiment. Nano CuO was first dispersed with 0.5% hydroxypropyl methyl cellulose (HPMC) and then diluted with normal saline to form the dispersive suspension. New Zealand rabbits are used in experiments. The rats were divided into 4080 nm and 200 nm nano-CuO groups (10 40 and 160 mg / L respectively) and 0.5 HPMC (dispersant control group, 3 animals in each group). The experiment was carried out according to the two schemes of no washing after exposure and washing at 4 s after exposure. No washing test: 0.1 ml nano-CuO test solution was dripped into the conjunctival sac of 3 rabbits, and the eyes were passively closed for 1 s, and the right eye was not treated for 4 s as a self-control flushing test: the rabbits were treated 4 seconds after exposure. The eyes were washed thoroughly with sterile saline for 30 s. The eyes of the animals were examined and scored on the 4th and 7th day after exposure. Results the conjunctiva hyperemia was observed in the 40 nm diameter three dose groups and the 80 nm 40 and 160 mg / L groups after exposure (1 h without washing). The symptoms of conjunctival hyperemia were alleviated at 48 and 72 hours after 24 h of progressive exacerbation at 80 nm and 40 nm nano-CuO, respectively. At the 4th day of observation, the hyperemia symptoms of all animals dissipated. No damage changes were observed in the cornea and iris of the above animals. No ocular irritation was observed in all dose groups. According to the results of acute eye irritation test, the irritation reaction of 40 nm and 80 nm nanocrystalline CuO eyes was classified as reversible eye injury. In the 4 s irrigation test, no obvious ocular irritation symptoms were found in all the animals in the 4080 and 200 nm groups. In the experiment, 0.5 HPMC was not eye irritating to all animals. Conclusion 40 nm and 80 nm nanocrystalline CuO particles have ocular irritation in conjunctival injury. Eye washing can effectively reduce the symptoms of acute eye irritation caused by nano Cu O contact.
【作者單位】： 中國疾病預(yù)防控制中心環(huán)境與健康相關(guān)產(chǎn)品安全所環(huán)境毒理室;北京工業(yè)大學(xué)環(huán)境與能源工程學(xué)院;
【基金】：中國疾病預(yù)防控制中心環(huán)境與健康相關(guān)產(chǎn)品安全所發(fā)展項(xiàng)目基金(FZ001)
【分類號】：R114
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本文編號：2046924