本文選題：榆黃菇 + 毛木耳��； 參考：《食品研究與開發(fā)》2017年08期

【摘要】：通過熱水提取、乙醇沉淀的方式獲得榆黃菇、毛木耳和灰樹花多糖,利用分子量截留的超濾方法獲得15個分級多糖組分。采用酒精和脂肪酸(FFA)共同誘導(dǎo)Hep G2細(xì)胞建立脂肪肝模型,以細(xì)胞存活率和胞內(nèi)甘油三酯含量為檢測指標(biāo)評價分級多糖消脂護(hù)肝作用。研究結(jié)果發(fā)現(xiàn)PS V、AP V和GF V 3個分子量高于100 k Da的組分是各自品種分級多糖中的優(yōu)勢組分,比例分別為71.06%、34%和68.89%;3種分級多糖可以提高肝損傷細(xì)胞的存活率分別為27.61%、37.68%和29.25%,同時減少甘油三酯在細(xì)胞內(nèi)的堆積,具有明顯的消脂護(hù)肝作用。
[Abstract]:Polysaccharides of Ulmus officinalis, Auricularia pubescens and Grifola frondosa were obtained by hot water extraction and ethanol precipitation, and 15 fractionated polysaccharides were obtained by ultrafiltration with molecular weight interception. The fatty liver model of HepG2 cells was induced by alcohol and fatty acid FFA.The survival rate and intracellular triglyceride content of HepG2 cells were used to evaluate the effect of fractionated polysaccharides on lipids and liver protection. The results showed that the three components with molecular weight of more than 100kDa were the dominant components in the classification of polysaccharides. The ratio of 71.06% and 68.89% were increased by 27.61% and 29.25%, respectively, and triglyceride accumulation in the cells was reduced, which had obvious antilipidemic and protective effects on the liver. The results showed that the lipopolysaccharide could increase the survival rate of the injured liver cells by 27.61% and 29.25% respectively, while reducing the accumulation of triglycerides in the cells.
【作者單位】： 北京市農(nóng)林科學(xué)院植物保護(hù)環(huán)境保護(hù)研究所北京市食用菌工程技術(shù)研究中心;農(nóng)業(yè)部都市農(nóng)業(yè)(北方)重點(diǎn)實(shí)驗(yàn)室;
【基金】：北京市優(yōu)秀人才培養(yǎng)資助項(xiàng)目(2015000020060G138) 北京市農(nóng)林科學(xué)院青年基金項(xiàng)目(QNJJ201524);北京市農(nóng)林科學(xué)院科技創(chuàng)新能力建設(shè)專項(xiàng)(KJCX20170205)
【分類號】：R151

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