本文選題：納米二氧化鈦 + 四氧嘧啶��； 參考：《西安交通大學(xué)學(xué)報(醫(yī)學(xué)版)》2015年04期

【摘要】：目的研究二氧化鈦(TiO2)納米材料對正常及氧化應(yīng)激SD大鼠腎組織的不良影響。方法通過肌肉注射四氧嘧啶,構(gòu)建氧化應(yīng)激模型,并分為四氧嘧啶處理組(OS組)、納米TiO2處理組(NM組)及四氧嘧啶與納米TiO2共處理組(OS-NM組),后兩組分別采用一次性腹腔注射0.5、5和50mg/kg體質(zhì)量納米TiO2的方式進行染毒,另設(shè)正常對照組。檢測氧化應(yīng)激指標(biāo)、尿素氮(BUN)水平并制備腎組織病理切片。結(jié)果注射5mg/kg體質(zhì)量納米TiO2時,OS-NM組大鼠O2-·和超氧化物歧化酶(SOD)較OS組和NM組增高,差異均有統(tǒng)計學(xué)意義(P0.05);當(dāng)注射50mg/kg體質(zhì)量納米TiO2時,與OS組及NM組分別比較,OS-NM組大鼠的O2-·、谷胱甘肽(GSH)和SOD水平變化差異均極顯著(P0.01);且OS組與NM組相比較,O2-·和GSH的變化有統(tǒng)計學(xué)意義(P0.05)。與染毒后的正常大鼠相比,納米TiO2導(dǎo)致氧化應(yīng)激大鼠BUN水平的異常升高(P0.01),并引起腎臟出現(xiàn)腎小球體積增大、細(xì)胞數(shù)目增多、腎小管上皮細(xì)胞水腫。方差分析表明四氧嘧啶與納米TiO2在導(dǎo)致O2-·水平升高的過程中存在協(xié)同作用。結(jié)論 TiO2納米材料對正常及氧化應(yīng)激大鼠腎臟均有損傷,且四氧嘧啶與納米TiO2協(xié)同作用能誘發(fā)更為嚴(yán)重的氧化應(yīng)激狀態(tài)從而加重對腎臟的損傷,這為患者安全使用納米材料提供了一定的實驗依據(jù)。
[Abstract]:Objective to study the adverse effects of titanium dioxide (TiO2) nanomaterials on the renal tissue of normal and oxidative stress SD rats. Methods the oxidative stress model was constructed by intramuscular injection of four oxouracil, and divided into four alloxan treatment group (group OS), nanoscale TiO2 treatment group (NM group) and four oximidine and nano TiO2 co processing group (group OS-NM), and the latter two groups were used respectively. One time intraperitoneal injection of 0.5,5 and 50mg/kg body mass nanoscale TiO2 was carried out, and the normal control group was set up. The oxidative stress index, the urea nitrogen (BUN) level and the renal tissue pathological section were detected. The results showed that the O2- and superoxide dismutase (SOD) of the OS-NM group was higher than that of the OS and NM groups when the mass of 5mg/kg body was injected into the nano TiO2. Statistical significance (P0.05); when the mass of 50mg/kg body was injected with nano TiO2, the difference of O2-, O2-, glutathione (GSH) and SOD in group OS-NM was very significant (P0.01) compared with group OS and NM group, and the change of OS group was statistically significant compared with that of NM group. The abnormal increase of BUN level in oxidative stress rats (P0.01) and the increase of glomerular volume, number of cells and edema of renal tubular epithelial cells were caused in the kidney. The analysis of variance analysis showed that four oxalacil and nano TiO2 have synergistic effect in the process of increasing the level of O2-. The TiO2 nanomaterials on normal and oxidative stress rats' kidneys The synergism between four pyrimidine and nanoscale TiO2 can induce more serious oxidative stress and aggravate the damage to the kidney, which provides a certain experimental basis for the safe use of nanomaterials.
【作者單位】： 西安醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)部基礎(chǔ)醫(yī)學(xué)研究所;西安交通大學(xué)醫(yī)學(xué)部第一附屬醫(yī)院麻醉科;
【基金】：國家自然科學(xué)基金青年項目(No.81301308,No.81301040) 陜西省自然科學(xué)基礎(chǔ)研究計劃青年項目(No.2014JQ4158) 陜西省青年科技新星項目(No.2014KJXX-76)~~
【分類號】：R114

【參考文獻】

相關(guān)期刊論文 前1條

1 魏海峰;李才;方艷秋;魏雁虹;譚巖;;單寧酸對高糖和糖化終末產(chǎn)物培養(yǎng)條件下腎小球系膜細(xì)胞氧化應(yīng)激及微炎癥狀態(tài)的改善作用[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2014年05期

【共引文獻】

相關(guān)期刊論文 前10條

1 付艷云;張艷秋;馬書梅;尹立紅;浦躍樸;梁戈玉;;納米二氧化鈦對大鼠NK細(xì)胞活性和相關(guān)細(xì)胞因子的影響[J];癌變.畸變.突變;2014年03期

2 王云;陳章健;巴特;濮吉;崔梟醒;賈光;;納米二氧化鈦對幼年和成年大鼠肝、腎組織抗氧化功能及元素含量的影響[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2014年03期

3 Chen Dawei;Pu Junhua;Tang Xiujun;Lu Junxian;Liu Yinyin;Jia Xiaoxu;Ge Qinglian;Gao Yushi;;Effects of Exposure to Lead and Cadmium on the Oxidative Damage of Livers in Laying Hens[J];Animal Husbandry and Feed Science;2014年05期

4 吳婕;徐晶;李云杰;謝紅軍;;二氧化鈦納米顆粒的成骨細(xì)胞毒性研究[J];口腔醫(yī)學(xué);2013年12期

5 王文斌;李倉廩;安麗紅;張鳳梅;辛菲;趙慧男;馮德達;李杰;李國珍;;納米二氧化鈦對大鼠氧化應(yīng)激水平及海馬組織結(jié)構(gòu)的影響[J];環(huán)境與健康雜志;2013年12期

6 劉衛(wèi)花;胡博驊;馮涓;錢怡;張文平;仇玉蘭;王偃倩;唐仕川;鄭金平;;納米碳酸鈣亞慢性染毒對大鼠神經(jīng)行為的影響[J];環(huán)境與職業(yè)醫(yī)學(xué);2014年05期

7 張陽;朱琳;;不同晶型納米TiO_2在斑馬魚體內(nèi)的富集與清除[J];環(huán)境科學(xué)研究;2015年01期

8 趙峰;孟幻;晏亮;汪冰;趙宇亮;;納米生物效應(yīng)與納米毒理學(xué)中的劑量與表面化學(xué)效應(yīng)關(guān)系（英文）[J];Science Bulletin;2015年01期

9 牛林紅;董會臺;楊麗莉;宋波;張榮;牛玉杰;;不同粒徑納米二氧化鈦對小鼠抗氧化能力的損傷作用[J];河北醫(yī)科大學(xué)學(xué)報;2015年02期

10 宋明芬;王玉文;王，

本文編號：2028141