本文選題：分子印跡聚合物 + 硝基酚類污染物��； 參考：《華中科技大學》2013年碩士論文

【摘要】：分子印跡技術（Molecular Imprinting Technique，MIT），是基于抗原/抗體的基本原理而延伸的分子識別技術。分子印跡聚合物(Molecularly Imprinted Polymers，MIPs)是在模板分子存在的情況下，運用分子印跡技術通過功能單體與交聯(lián)劑的共聚反應制得，去除模板分子后，分子印跡聚合物上可形成與模板分子在空間結構上相匹配的三維孔穴，可特異性地吸附模板分子及其結構類似物，因此也被稱為“人工抗體”。本文采用沉淀聚合的方法，獲得了對硝基酚類污染物具有特異性識別作用的親水性分子印跡聚合物（MIPs），并實現(xiàn)水體中痕量硝基酚污染物的選擇性萃取及去除。主要研究內容由以下兩部分組成： 1.為了克服傳統(tǒng)固相萃取所具有的缺點，本研究采用親水性MIPs-固相微柱萃取技術對水樣（東湖，長江，工業(yè)廢水）中硝基酚類污染物進行萃取。固相微柱萃取技術不需要傳統(tǒng)的萃取裝置及蒸發(fā)等復雜操作流程，僅通過離心便可完成對樣品的上樣、淋洗和洗脫，具有操作簡單、快速的優(yōu)點。在最佳萃取條件下，親水性MIPs-固相微萃取柱對硝基酚類化合物的萃取回收率達到87%-92.9%，且具有良好的凈化效果。與常規(guī)的萃取吸附劑（活性炭、多壁碳納米管、LC-C18等）相比，，親水性MIPs顯示出更高的萃取效率和更好的選擇性識別性能。本研究建立親水性MIPs-固相微柱萃取結合HPLC-UV法對實際水樣中硝基酚類污染物進行檢測，結果顯示：該方法的線性范圍為2-1000ng/mL（2,4-二硝基酚，2-硝基酚）、6-1000ng/mL（4-硝基酚），相關系數(shù)（R2）大于0.998，方法檢測限為0.3-0.5ng/mL,日內RSDs值為4.0%-6.7%，日間（5日）RSDs值為5.9%-8.2%。 2.將親水性MIPs作為吸附劑對地表水及工業(yè)廢水中2,4-二硝基酚進行選擇性去除研究。吸附動力學模型及吸附等溫模型結果顯示，親水性MIPs對2,4-二硝基酚的最大吸附容量為138.9mg/g，同時，進一步研究了吸附過程中水樣的流速、初始濃度、pH和腐殖酸含量對去除效果的影響。大體積上樣時（上樣體積高達1L），與活性炭、多壁碳納米管、LC-C18等常規(guī)吸附柱及常規(guī)方法制備的MIPs相比，親水性MIPs（100mg）具有最好的去除效率（92%），并可重復使用5次以上，具有較好的實際應用前景。
[Abstract]:Molecular imprinting technique is an extended molecular recognition technique based on the basic principle of antigen / antibody. Molecularly imprinted polymers (MIPs) were prepared by the copolymerization of functional monomers and crosslinkers in the presence of template molecules, and the template molecules were removed. Molecular imprinted polymers can form three-dimensional pores that match the spatial structure of template molecules, and can specifically adsorb template molecules and their structural analogues, so they are also called "artificial antibodies". In this paper, a hydrophilic molecularly imprinted polymer (MIPsN) with specific recognition effect on nitrophenols was obtained by precipitation polymerization, and the selective extraction and removal of trace nitrophenol pollutants in water were realized. The main research content consists of the following two parts: 1. In order to overcome the shortcomings of traditional solid phase extraction (SPE), the hydrophilic MIPs- solid phase microcolumn extraction (SPME) technique was used to extract nitrophenols from water samples (Donghu, Yangtze River, industrial wastewater). Solid-phase microcolumn extraction technology does not need the traditional extraction equipment and evaporation and other complex operation flow, only by centrifugation can be completed on the sample, elution and elution, has the advantages of simple operation, rapid operation. Under the optimum extraction conditions, the extraction recovery of hydrophilic MIPs- solid phase microextraction column was 87-92. 9%, and it had a good purification effect. Compared with conventional extraction adsorbents (activated carbon, multi-walled carbon nanotubes, LC-C18, etc.), hydrophilic MIPs show higher extraction efficiency and better selective recognition performance. A hydrophilic MIPssolid phase microcolumn extraction (SPME) method combined with HPLC-UV was established for the determination of nitrophenols in real water samples. The results showed that the linear range of the method was 2-1000ng / mL ~ (2) -dinitrophenol ~ (2-nitrophenol) = 6-1000ng / m ~ (-1) N ~ (-1) 路m ~ (-1) N ~ (4-nitrophenol) > 0.998, the detection limit was 0.3-0.5ng 路mL ~ (-1), the value of RSDs within day was 4.0-6.7, and the value of RSDs was 5.9- 8.22. Hydrophilic MIPs were used as adsorbents for selective removal of 2-trichloro-4-dinitrophenol from surface water and industrial wastewater. The results of adsorption kinetics model and adsorption isotherm model show that the maximum adsorption capacity of hydrophilic MIPs for 2o 4-dinitrophenol is 138.9 mg / g. At the same time, the flow rate of water sample during the adsorption process is further studied. Effects of initial concentration pH and humic acid content on removal efficiency. The hydrophilic MIPsn 100mgcompared with conventional adsorption columns such as activated carbon, multi-walled carbon nanotube LC-C18 and MIPs prepared by conventional methods, has the best removal efficiency and can be reused for more than 5 times. It has a good prospect of practical application.
【學位授予單位】：華中科技大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R123.1

【參考文獻】

相關期刊論文 前1條

1 張繼義;王利平;常玉枝;;麥草對水中苯胺的吸附平衡、熱力學和動力學研究[J];農(nóng)業(yè)系統(tǒng)科學與綜合研究;2011年03期

本文編號：2009194