本文選題：恩諾沙星 + 免疫層析技術(shù)��； 參考：《食品科學(xué)》2017年22期

【摘要】：目的:結(jié)合膠體金標(biāo)記技術(shù)和免疫層析技術(shù)建立膠體金免疫層析毛細(xì)管檢測(cè)技術(shù),用于水產(chǎn)品中恩諾沙星殘留的快速現(xiàn)場(chǎng)檢測(cè)。方法:以間接競(jìng)爭(zhēng)的實(shí)驗(yàn)原理,通過(guò)優(yōu)化檢測(cè)區(qū)抗原、質(zhì)控區(qū)二抗、金標(biāo)一抗檢測(cè)濃度初步建立膠體金免疫層析毛細(xì)管檢測(cè)方法,并進(jìn)一步確定其檢測(cè)靈敏度、特異性、重復(fù)性等及前處理的優(yōu)化操作。結(jié)果:該方法檢測(cè)恩諾沙星的視覺(jué)檢測(cè)限為5 ng/mL,半定量檢測(cè)限為1.29 ng/mL。在大菱鲆、鲅魚(yú)、舌鰨、南美白對(duì)蝦陰性樣品中的平均添加回收率在78.3%~129%之間,相對(duì)標(biāo)準(zhǔn)偏差均小于10%。特異性實(shí)驗(yàn)表明,除對(duì)環(huán)丙沙星外,對(duì)其他相似物的特異性較好;環(huán)丙沙星的視覺(jué)檢測(cè)限為10 ng/mL,半定量檢測(cè)限為4.37 ng/mL,滿足國(guó)標(biāo)檢測(cè)的限量要求。同時(shí),針對(duì)免疫層析毛細(xì)管檢測(cè)的前處理方法進(jìn)行初步探究,最終確定采用常溫條件下磺基水楊酸提取的前處理方法。結(jié)論:恩諾沙星膠體金免疫層析毛細(xì)管檢測(cè)技術(shù)具有簡(jiǎn)單、快速、易操作、重復(fù)性好的優(yōu)勢(shì),為食品藥物殘留檢測(cè)領(lǐng)域提供了檢測(cè)新方法。同時(shí),選擇新型試劑作為膠體金免疫層析毛細(xì)管檢測(cè)技術(shù)的前處理方法,該方法可以為多種快檢技術(shù)的前處理提供理論基礎(chǔ)與技術(shù)支撐。
[Abstract]:Objective: to establish a capillary detection technique for the detection of enrofloxacin residues in aquatic products by using colloidal gold labeling technique and immunochromatographic technique. Methods: based on the principle of indirect competition, the capillary detection method of colloidal gold immunochromatography was established by optimizing the detection concentration of antigen, second antibody in quality control area and first antibody in gold standard, and the detection sensitivity and specificity were further determined. Repetitive and pre-processing optimization operation. Results: the visual detection limit and semi-quantitative detection limit of enrofloxacin were 5 ng / mL and 1.29 ng / mL, respectively. The average recovery rate in negative samples of turbot, Spanish mackerel, sole and Penaeus vannamei was 78.3% and 12.9%, and the relative standard deviation was less than 10%. The specificity of ciprofloxacin was better than that of ciprofloxacin, and the visual detection limit of ciprofloxacin was 10 ng / mL and the semi-quantitative detection limit was 4.37 ng / mL. At the same time, the preprocessing method of capillary detection by immunochromatography was preliminarily explored, and the pretreatment method of sulfosalicylic acid extraction at room temperature was finally determined. Conclusion: the capillary detection technique of enrofloxacin colloidal gold immunochromatography is simple, rapid, easy to operate and reproducible, which provides a new method for the detection of food and drug residues. At the same time, a new reagent is chosen as the pretreatment method of colloidal gold immunochromatographic capillary detection technology, which can provide theoretical basis and technical support for the pretreatment of many rapid detection techniques.
【作者單位】： 中國(guó)海洋大學(xué)食品安全實(shí)驗(yàn)室;
【分類號(hào)】：R155.5

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本文編號(hào)：1993208