本文選題：果蔬發(fā)酵液 + 酒精肝損傷��； 參考：《營養(yǎng)學(xué)報》2015年04期

【摘要】：目的研究果蔬發(fā)酵液(vegetables and fruits ferment liquid,VFFL)對小鼠酒精性肝損傷的保護作用及其氧化應(yīng)激機制。方法 50只雄性ICR小鼠隨機分為正常對照組、乙醇模型組、果蔬發(fā)酵液組[10、30、60ml/(kg·d)],正常對照組和乙醇模型組蒸餾水灌胃,其余組用不同劑量果蔬發(fā)酵液灌胃,連續(xù)30d,最后一次灌胃16h后,乙醇模型組和各果蔬發(fā)酵液組均1次性50%乙醇12ml/kg·bw灌胃,6h后處死、取血和肝組織。分別采用HE染色觀察肝組織,試劑盒測定血清中谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、堿性磷酸酶(AKP)含量及肝組織超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽過氧化物酶(GSH-Px)、丙二醛(MDA)、蛋白質(zhì)羰基、活性氧(ROS)含量,TUNEL法測定肝細胞凋亡,蛋白免疫印跡法檢測凋亡相關(guān)蛋白表達。結(jié)果與乙醇模型組比較,果蔬發(fā)酵液組的肝組織水腫和壞死明顯減輕;血清中的ALT、AST、AKP水平和肝細胞凋亡明顯低于乙醇模型組(P0.05);肝組織中SOD、GSH、GSH-Px水平明顯高于乙醇模型組(P0.05),而ROS、MDA、蛋白質(zhì)羰基含量明顯低于乙醇模型組(P0.05);果蔬發(fā)酵液組的VDAC(電壓依賴性陰離子通道蛋白)、Bcl-2蛋白表達增高(P0.05),細胞色素C、caspase-9、caspase-3、Bax蛋白表達下降(P0.05)。結(jié)論果蔬發(fā)酵液具有較好的抗氧化作用,它可通過阻止ROS介導(dǎo)的線粒體信號通路,抑制肝細胞凋亡,從而保護小鼠酒精性肝損傷。
[Abstract]:Objective to study the protective effect of vegetable and fruits ferment liquid (VFFLL) on alcoholic liver injury in mice and the mechanism of oxidative stress. Methods Fifty male ICR mice were randomly divided into three groups: normal control group, ethanol model group, fruit and vegetable fermentation broth group [1030ml / kg / d], normal control group and ethanol model group were fed with distilled water, and other groups were fed with different doses of fruit and vegetable fermentation liquid. For 30 consecutive days, after 16 hours of the last oral administration, 50% ethanol 12ml/kg BW was given once for 6 hours in the ethanol model group and every fruit and vegetable fermentation broth group, and the blood and liver tissues were taken out. Liver tissues were observed by HE staining. Determination of serum alanine aminotransferase (alt), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and liver tissue superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-PxN), malondialdehyde (MDAA), protein carbonyl, The content of reactive oxygen species (Ros) was determined by Tunel and the expression of apoptosis-related proteins was detected by Western blot. Results compared with the ethanol model group, the edema and necrosis of liver tissue in the fruit and vegetable fermentation broth group were obviously alleviated. The levels of AKP and apoptosis of liver cells in serum were significantly lower than those in ethanol model group, and the level of GSH-Px in liver tissue was significantly higher than that in ethanol model group, while the content of protein carbonyl group in ROSN MDAs was significantly lower than that in ethanol model group, and the VDAC5 (voltage dependent on voltage) in fruit and vegetable fermentation broth group was significantly lower than that in ethanol model group. The expression of Bcl 2 protein increased and the expression of cytochrome Caspase-9, caspase-3, Bax protein decreased (P 0.05). Conclusion Fruit and vegetable fermentation broth has good antioxidation effect. It can inhibit hepatocyte apoptosis by blocking mitochondrial signaling pathway mediated by ROS and thus protect mice from alcoholic liver injury.
【作者單位】： 南京醫(yī)科大學(xué)公共衛(wèi)生學(xué)院營養(yǎng)與食品衛(wèi)生系;中國食品發(fā)酵工業(yè)研究院;
【基金】：江蘇高校優(yōu)勢學(xué)科建設(shè)工程資助項目(2011)
【分類號】：R151

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