本文選題：虱螨脲原藥 + 亞慢性毒性　； 參考：《毒理學雜志》2015年06期

【摘要】：目的研究虱螨脲原藥主要亞慢性經口毒性作用,取得虱螨脲原藥亞慢性經口的最大無作用劑量和最小有作用劑量參數(shù),初步確定毒作用的靶器官。方法采用經口喂飼法染毒,設3個染毒劑量組和1個空白對照組,雌、雄各半,分別用含500、2 000和8 000 mg/kg虱螨脲原藥的加藥飼料對大鼠連續(xù)經口喂飼染毒90 d,觀察動物的一般表現(xiàn)、體重、進食量,試驗結束時進行血常規(guī)、尿常規(guī)、血生化指標、臟器重量、臟器系數(shù)以及病理組織學檢查。結果血常規(guī)檢查結果顯示,中、高劑量組雌鼠白細胞計數(shù)(WBC)降低(P0.05和0.01),雄鼠白細胞計數(shù)(WBC)、淋巴細胞比例(LYM)降低(P0.01)、中性粒細胞比例(MON)增高(P0.01),高劑量組雄鼠單核細胞(GRA)比例明顯增高(P0.05);血液生化檢查結果顯示,高劑量組雌鼠尿素氮(BUN)、肌酐(CREA)升高(P0.01),中、高劑量組雄鼠總膽固醇(CHO)、尿素氮(BUN)增高(P0.01);臟器系數(shù)檢查結果顯示,中、高劑量組雌鼠肝體比值增高(P0.05和0.01),雄鼠肝體比值增高(P0.01),睪丸及睪丸體重比值降低(P0.05);病理組織學檢查結果顯示,中、高劑量組雄鼠睪丸曲精小管生精上皮變性、壞死或消失、睪丸萎縮、間質增生,睪丸病變發(fā)生率增高(P0.05);高劑量組雄鼠肝臟脂肪變性發(fā)生率增加(P0.01);各劑量組尿常規(guī)檢查結果顯示與對照組比較,差異無統(tǒng)計學意義(P0.05)。低劑量組雌、雄鼠各項指標與其對照組相比,均未見明顯差異或有臨床意義的改變。結論虱螨脲原藥對大鼠的血液、肝、腎、生殖器官、神經系統(tǒng)具有毒性作用。其對SD大鼠亞慢性(90 d)經口毒性的最大無作用劑量雌、雄分別為為41.4和37.9 mg/kg、最小有作用劑量雌、雄分別為為152.0和147.8 mg/kg。
[Abstract]:Objective to study the main subchronic oral toxicity of Licia ureas, obtain the maximum non action dose and minimum dose parameter of the subchronic oral acaron drug, initially determine the target organ of the toxic effect. Methods by oral feeding, 3 dose groups and 1 blank control groups were set up, female and male were half with 500,2 00, respectively. 0 and 8000 mg/kg acarin feed was fed to rats for 90 d. The general performance, weight, food intake, blood routine, urine routine, blood biochemical index, organ weight, organ coefficient and histopathology examination were observed at the end of the test. Results the results of blood routine examination showed that in the high dose group, the female mice white were white. Cell count (WBC) decreased (P0.05 and 0.01), white cell count (WBC), lymphocyte proportion (LYM) decreased (P0.01), neutrophils ratio (MON) increased (P0.01), and the proportion of mononuclear cells (GRA) in high dose group increased significantly (P0.05). The results of blood biochemical examination showed that the urea nitrogen (BUN) and creatinine (CREA) increased (P0.01) in the high dose group of female rats (P0.01), in the high dose group. In high dose group, the total cholesterol (CHO) and urea nitrogen (BUN) increased (P0.01). The results of organ coefficient examination showed that the ratio of liver body increased (P0.05 and 0.01) in the high dose group (P0.05 and 0.01), the ratio of liver body in male rats increased (P0.01) and the ratio of testis and testis decreased (P0.05). Histopathological examination showed that the high dose group of male rat testis testis tubule tubule Degeneration, necrosis or disappearance of spermatogenic epithelium, atrophy of testis, interstitial hyperplasia and higher incidence of testicular disease (P0.05); the incidence of fatty degeneration in the liver of male rats increased (P0.01) in high dose group (P0.05). The results of urine routine examination in each dose group showed no significant difference between the control group and the control group. Conclusion there were toxic effects on the blood, liver, kidney, reproductive organs and nervous system of rats. The maximum dose of male and male of the subchronic (90 d) SD rats were 41.4 and 37.9 mg/kg, the smallest dose was 152 and 147., respectively. 8 mg/kg.
【作者單位】： 湖南省職業(yè)病防治院;
【分類號】：R114

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