本文選題：辣椒素 + 血脂　； 參考：《西南大學(xué)》2012年碩士論文

【摘要】：本文以辣椒素為原料,通過化學(xué)分析、儀器分析和動(dòng)物模型建立,對(duì)辣椒素對(duì)大鼠體內(nèi)膽固醇代謝機(jī)理進(jìn)行了研究。為辣椒素在食品加工和飲食健康方面的應(yīng)用提供了理論依據(jù)。其研究主要結(jié)論如下： (1)采用32只SD大鼠,雌雄各半,適應(yīng)性飼養(yǎng)一周后隨機(jī)分為四組,空白組、低劑量組、中劑量組、高劑量組,每天分別灌胃0、2.5、5.0、7.5mg/kg辣椒素,基礎(chǔ)飼料飼養(yǎng)28天。經(jīng)檢測發(fā)現(xiàn)辣椒素能降低大鼠的體重,對(duì)飼料效率沒有顯著性影響。辣椒素能降低大鼠血漿和肝臟中膽固醇含量,這種效果存在性別差異,雌性大鼠對(duì)辣椒素功效的敏感性高于雄性。辣椒素能降低大鼠肝臟重量、肝臟脂肪含量、肝臟中總膽固醇和甘油三酯總含量。高劑量辣椒素使肝臟內(nèi)TRPV1的mRNA表達(dá)量增加,使CYP7A1、FXR和HMG-CoA的mRNA表達(dá)量降低(P0.05),提高大鼠回腸中ASBT和IBABP mRNA的表達(dá)量(P0.05)。本實(shí)驗(yàn)證明每天灌胃高劑量組辣椒素對(duì)刺激大鼠食欲,降低大鼠血脂和肝脂,影響膽固醇代謝循環(huán)有較好效果。辣椒素通過降低肝臟膽固醇代謝基因和升高回腸膽汁酸代謝基因,降低體內(nèi)血脂、肝脂,從而調(diào)節(jié)膽固醇在體內(nèi)的代謝循環(huán)。 (2)模擬更年期婦女建立動(dòng)物模型。采用30只雌性SD大鼠,分組進(jìn)行雙側(cè)卵巢切除(OVX)手術(shù)和偽切除手術(shù), OVX組大鼠隨機(jī)分成4組,空白組、低劑量組、中劑量組、高劑量組,每天分別灌胃0、5.0、10.0、15.0mg/kg辣椒素,喂食相同基礎(chǔ)飼料28天。經(jīng)檢測發(fā)現(xiàn),OVX空白組大鼠的體重、采食量、血脂、肝臟總脂肪、總膽固醇、甘油三酯總含量均顯著高于偽切除組,說明造模成功。辣椒素可降低OVX組大鼠的體重增加量、進(jìn)食量,對(duì)飼料效率沒有影響；辣椒素能降低血漿和肝臟中膽固醇濃度；降低大鼠肝臟中脂肪、總TC和總TG含量。辣椒素能升高肝臟中TRPV1的mRNA表達(dá)(P0.05),降低肝臟中CYP7A1、FXR和HMG-CoA的mRNA表達(dá)(P0.05)；辣椒素能提高大鼠回腸中TRPV1、ASBT和IBABP mRNA的表達(dá)(P0.05)。本實(shí)驗(yàn)證明,辣椒素對(duì)因雌性激素缺乏引起高脂血癥雌性大鼠具有較好的降血脂、降肝脂和調(diào)節(jié)膽固醇代謝的效果,能有效預(yù)防雌性大鼠因雌激素缺乏引起的高脂血癥的發(fā)生。辣椒素通過降低高脂血癥大鼠肝臟內(nèi)膽固醇代謝基因,升高回腸膽汁酸代謝基因,降低體內(nèi)血脂、肝脂,從而調(diào)節(jié)膽固醇在體內(nèi)的代謝循環(huán)。 (3)采用48只SD大鼠,雌雄各半,隨機(jī)分成六組,分別喂食基礎(chǔ)、高脂和高膽固醇飼料,每種飼料組又分為灌胃辣椒素組(10mg/kg/d)和不灌胃辣椒素組。 與基礎(chǔ)飼料組相比,高脂飼料組大鼠血脂和肝脂顯著升高(P0.05),肝臟重量也有升高趨勢。肝臟中TRPV1、HMG-CoA的mRNA表達(dá)量顯著降低(P0.05)；CYP7A1和FXR的mRNA表達(dá)量顯著升高(P0.05),雌性大鼠肝臟中FXR的mRNA表達(dá)量沒有顯著變化,但有升高趨勢。高脂飼料組大鼠回腸中的TRPV1的mRNA顯著升高(P0.05),ASBT和IBABP的mRNA顯著降低(P0.05)。辣椒素可顯著降低高脂飼料組大鼠的血脂和肝脂,升高雄性大鼠肝臟中TRPV1的mRNA的表達(dá)量(P0.05),降低FXR和CYP7A1的mRNA的表達(dá)量(P0.05),對(duì)HMG-CoA的mRNA沒有顯著性影響,但是有降低趨勢。顯著升高雌性大鼠肝臟中TRPV1的表達(dá)量(P0.05),降低FXR、HMG-CoA和CYP7A1的mRNA表達(dá)量(P0.05)。升高大鼠回腸中TRPV1、ASBT、IBABP的mRNA的表達(dá)量。 與基礎(chǔ)飼料組相比,高膽固醇飼料組大鼠血脂和肝脂升高,肝臟重量也有升高趨勢。肝臟中的TRPV1和HMG-CoA的mRNA的表達(dá)量顯著降低(P0.05),FXR和CYP7A1的mRNA的表達(dá)量顯著升高(P0.05),回腸中ASBT和IBABP的mRNA的表達(dá)量顯著降低(P0.05),雌性大鼠回腸中的TRPV1的mRNA顯著升高(P0.05)；雄性大鼠回腸中TRPV1的mRNA沒有顯著變化。辣椒素可降低高膽固醇飼料組大鼠的血脂和肝脂,升高肝臟中TRPV1的mRNA的表達(dá)量(P0.05),顯著降低FXR、HMG-CoA和CYP7A1的mRNA的表達(dá)量(P0.05)；顯著升高高膽固醇飼料組大鼠回腸中TRPV1、ASBT和IBABP的mRNA表達(dá)量(P0.05)。 本實(shí)驗(yàn)證明,辣椒素能有效降低喂食高脂飼料和高膽固醇飼料大鼠的血脂和肝脂,并能有效調(diào)節(jié)體內(nèi)膽固醇代謝循環(huán)�？梢�,辣椒素能有效預(yù)防高脂飼料和高膽固醇飼料引起的高脂血癥。辣椒素通過降低高脂血癥大鼠肝臟內(nèi)膽固醇代謝基因和升高回腸膽汁酸代謝基因,降低體內(nèi)血脂、肝脂,從而調(diào)節(jié)膽固醇在體內(nèi)的代謝循環(huán)。
[Abstract]:With capsaicin as raw material, the mechanism of capsaicin's cholesterol metabolism in rats was studied by chemical analysis, instrumental analysis and animal model. The theoretical basis for the application of capsaicin in food processing and diet health was provided.
(1) 32 SD rats and male and female rats were divided into four groups after one week of adaptation. The blank group, the low dose group, the middle dose group and the high dose group were fed with capsaicin 0,2.5,5.0,7.5mg/kg and the basal diet for 28 days. The capsaicin could reduce the weight of the rats and had no significant effect on the feed efficiency. Capsaicin could decrease. The cholesterol content in the plasma and liver of the rats had a gender difference. The sensitivity of the female rats to capsaicin was higher than that in the male. Capsaicin could reduce the liver weight, liver fat content, total cholesterol and triglyceride content in the liver. High dose capsicum increased the mRNA expression of TRPV1 in the liver, and made CYP7A1, The mRNA expression of FXR and HMG-CoA decreased (P0.05) and increased the expression of ASBT and IBABP mRNA in the ileum of rats (P0.05). This experiment proved that capsaicin has a good effect on stimulating rats' appetite, lowering blood lipid and liver fat, and affecting the cholesterol metabolism cycle. Capsaicin can reduce the cholesterol metabolism gene and increase in liver. Ileal bile acid metabolism genes reduce blood lipids and liver fat, thereby regulating the metabolic cycle of cholesterol in the body.
(2) to simulate the animal model of the menopause women. 30 female SD rats were used to perform bilateral ovariectomy (OVX) surgery and pseudo excision. Group OVX rats were randomly divided into 4 groups, blank group, low dose group, medium dose group, high dose group, respectively, respectively, and fed with 0,5.0,10.0,15.0mg/kg capsaicin every day, feeding the same basic feed for 28 days. The test was tested. The total content of weight, feed intake, blood lipid, total fat, total cholesterol and triglyceride in the OVX blank group were significantly higher than that in the pseudo excision group, indicating that the model was successful. Capsaicin could reduce the weight gain of rats in group OVX, and had no effect on the feed efficiency; capsaicin could reduce the concentration of cholesterol in the plasma and liver; reduce the large amount of cholesterol in the liver. Capsaicin can increase the mRNA expression of TRPV1 in the liver (P0.05), and reduce the mRNA expression of CYP7A1, FXR and HMG-CoA in the liver (P0.05). Capsaicin can improve the expression of TRPV1, ASBT, and HMG-CoA in the ileum of rats. It is proved that capsaicin can cause hyperlipidemia female with estrogen deficiency. The effect of reducing blood lipid, reducing liver fat and regulating cholesterol metabolism can effectively prevent the occurrence of hyperlipidemia caused by estrogen deficiency in female rats. Capsaicin can reduce the metabolism of bile acids in the ileum by lowering the cholesterol metabolism gene in the liver of hyperlipidemia rats, reduce the blood lipid and liver fat, and regulate the cholesterol. The metabolic cycle in the body.
(3) 48 SD rats and male and female rats were divided into six groups randomly. They were fed on the base, high fat and high cholesterol diet, and each feed group was divided into capsaicin group (10mg/kg/d) and no capsaicin group.
Compared with the basic diet group, the lipid and liver fat in the high fat diet group increased significantly (P0.05), the liver weight also increased. The mRNA expression of TRPV1 and HMG-CoA in the liver was significantly decreased (P0.05), the mRNA expression in CYP7A1 and FXR increased significantly (P0.05), but the mRNA expression of FXR in the liver of the female rats was not significantly changed, but there was a rising trend. The mRNA of TRPV1 in the ileum of the high fat diet group increased significantly (P0.05), and the mRNA of ASBT and IBABP decreased significantly (P0.05). Capsaicin significantly reduced the blood lipid and liver fat of the rats in the high fat diet group and increased the mRNA expression of TRPV1 in the liver of the male rats (P0.05), and decreased the expression of FXR and CYP7A1. The expression of TRPV1 (P0.05) in the liver of female rats and the mRNA expression of FXR, HMG-CoA and CYP7A1 (P0.05) were significantly increased in the female rat liver. The mRNA expression of TRPV1, ASBT, IBABP in the ileum was increased.
In the high cholesterol diet group, the blood lipid and liver fat increased and the liver weight also increased. The expression of TRPV1 and HMG-CoA in the liver decreased significantly (P0.05), the expression of mRNA in FXR and CYP7A1 increased significantly (P0.05), the mRNA expression of ASBT and IBABP in the ileum was significantly reduced (P0.05), female rats returned. The mRNA of TRPV1 in the intestine increased significantly (P0.05), and the mRNA of the TRPV1 in the ileum of the male rats was not significantly changed. The capsaicin reduced the lipid and liver fat of the high cholesterol diet group, increased the mRNA expression of the TRPV1 in the liver (P0.05), significantly reduced the expression of FXR, HMG-CoA and CYP7A1 mRNA, and significantly increased the high cholesterol feed. The expression of TRPV1, ASBT and IBABP mRNA in the ileum of rats (P0.05).
Capsaicin can effectively reduce blood lipid and liver lipids in high fat feed and high cholesterol diet rats, and can effectively regulate cholesterol metabolism in the body. Capsaicin can effectively prevent hyperlipidemia caused by high fat diet and high cholesterol diet. Capsaicin can reduce the cholesterol metabolism in the liver of rats with hyperlipidemia. Genes and increased ileal bile acid metabolism genes reduce body fat and liver fat, thereby regulating the metabolic cycle of cholesterol in the body.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R151

【引證文獻(xiàn)】

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2 桂余;唐艷;張端莉;劉雄;陳厚榮;;薯渣纖維素粒度對(duì)去勢大鼠腸道健康的影響[J];食品工業(yè)科技;2013年18期

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1 張磊;辣椒堿降大鼠膽固醇效果及其分子機(jī)理的研究[D];西南大學(xué);2013年

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1 彭林;柚皮膳食纖維提取技術(shù)及其功能特性的研究[D];西南大學(xué);2013年

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本文編號(hào)：1941161