本文選題：副溶血性弧菌 + 流行株　； 參考：《中國病原生物學(xué)雜志》2015年06期

【摘要】：目的了解上海市嘉定區(qū)副溶血性弧菌腹瀉患者和食品分離株的血清型、毒力基因和分子分型特征及其流行狀況。方法對2014年分離自腹瀉患者和食品的菌株進(jìn)行血清分型,以PCR檢測菌株的毒力基因tdh和trh及大流行株分子標(biāo)識GS-PCR和orf8基因,通過PFGE分型分析菌株間的遺傳關(guān)系。結(jié)果嘉定區(qū)副溶血性弧菌導(dǎo)致的腹瀉病例主要集中在5~10月份,高峰出現(xiàn)在8月份,患者年齡分布呈雙峰現(xiàn)象,女性感染者中檢出弱產(chǎn)毒或不產(chǎn)毒菌株的比例高于男性。腹瀉患者分離株中居前7位的血清型分別為O3:K6、O4:K9、O4:K8、O4:K68、O4:K12、O1:KUT、O3:KUT,產(chǎn)毒株占92.3%,流行株占61.9%,主要以O(shè)3:K6為主,orf8基因陰性流行株約占流行株的20.1%。323株副溶血性弧菌分為121個PFGE型,大流行株之間遺傳距離更為接近,均處于同一聚類單元;非流行株構(gòu)成復(fù)雜,形成以O(shè)4:K8、O4:K9、O4:K12、O3:K29(O3:KUT)為中心的4個聚類單元,且與大流行株距離較遠(yuǎn),非流行株之間在PFGE條帶上差異也較大。食品分離株均為非產(chǎn)毒株,未發(fā)現(xiàn)患者分離株和食品分離株具有相同的PFGE型,且未見形成明顯的PFGE聚類單元。結(jié)論副溶血性弧菌是嘉定區(qū)重要的腹瀉病原,其感染控制應(yīng)以大流行株和產(chǎn)毒株為主,食品監(jiān)測應(yīng)充分考慮副溶血性弧菌在病例中的分布情況。
[Abstract]:Objective to investigate the serotype, virulence gene and molecular typing of Vibrio parahaemolyticus from diarrhea patients and food isolates in Jiading District, Shanghai. Methods Serum typing of strains isolated from diarrhea patients and food in 2014 was carried out. The virulence genes tdh and trh of the strains were detected by PCR and the GS-PCR and orf8 genes were identified by the molecular markers of the pandemic strains. The genetic relationship between the strains was analyzed by PFGE typing. Results the diarrhea cases caused by Vibrio parahaemolyticus in Jiading district were mainly concentrated in May and October, the peak occurred in August, and the age distribution of the patients was double peak. The proportion of weakly producing or non-virulent strains detected in female infected persons was higher than that in male. The first 7 serotypes of diarrhea patients were O3: K6O4: K9O4: K8: K8O4: K68 / O4: K12O1: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUTO3: KUT@@ The genetic distance among the pandemic strains was closer and all were located in the same cluster unit, and the non-endemic strains were complicated and formed four cluster units with the center of O4: K8, O4: K9, O4: K12O3: K29: O3KUT.Moreover, they were far away from the pandemic strains, and the differences between the non-endemic strains on the PFGE strip were also great. All the food isolates were non-virulent, and no PFGE type was found in the patient and food isolates, and no obvious PFGE cluster unit was found. Conclusion Vibrio parahaemolyticus is an important pathogen of diarrhea in Jiading district, and the distribution of Vibrio parahaemolyticus in cases should be considered in food monitoring.
【作者單位】： 上海市嘉定區(qū)疾病預(yù)防控制中心;上海市疾病預(yù)防控制中心;
【基金】：上海青年醫(yī)師培養(yǎng)資助計劃(滬衛(wèi)人事[2012]114號)依托項目
【分類號】：R155.3

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本文編號：1939856