本文選題：四氯化碳 + 二乙基亞硝胺��； 參考：《動(dòng)物學(xué)雜志》2015年02期

【摘要】：為了研究四氯化碳(CCL4)聯(lián)合二乙基亞硝胺(DEN)對(duì)小鼠(Mus musculus domesticus)精巢的毒性作用。將昆明小鼠隨機(jī)分為對(duì)照組與實(shí)驗(yàn)組,各12只個(gè)體。實(shí)驗(yàn)組每周2次腹腔注射含有15%CCl4的花生油溶液,同時(shí)自由飲用0.07‰的DEN溶液,連續(xù)誘導(dǎo)8周。對(duì)照組每周2次注射花生油,飲用蒸餾水。分別于第3、6、8周處死實(shí)驗(yàn)組及對(duì)照組小鼠各4只,對(duì)其精巢組織進(jìn)行常規(guī)固定、包埋、切片、H.E染色,觀察精巢的形態(tài)及精子數(shù)量的變化。與實(shí)驗(yàn)組比較,對(duì)照組小鼠體重明顯增加,且實(shí)驗(yàn)組與對(duì)照組差異顯著;與對(duì)照組相比,實(shí)驗(yàn)組睪丸系數(shù)以及精子存活率均顯著減少。對(duì)照組小鼠精巢都具有正常的生精小管結(jié)構(gòu),管腔中存在大量精子;第3周,實(shí)驗(yàn)組小鼠的精巢與對(duì)照組類似,生精小管較為完整,生精細(xì)胞稍有散亂;第6周,實(shí)驗(yàn)組生精小管變形,生精細(xì)胞排列疏松,精子數(shù)目減少且畸形;第8周,實(shí)驗(yàn)組生精小管殘缺不全,生精細(xì)胞和精子散亂排列,精子數(shù)量大大減少且變形更為嚴(yán)重。實(shí)驗(yàn)說(shuō)明,CCl4聯(lián)合DEN能夠造成小鼠精巢組織的損害以及精子數(shù)目的減少和畸變。
[Abstract]:In order to study the toxic effects of CCL4 (CCL4) combined with diethylnitrosamine (DEN) on the spermatozoa of mice (Mus musculus domesticus). Kunming mice were randomly divided into control group and experimental group, each with 12 individuals. In the experimental group, peanut oil solution containing 15l4 was injected intraperitoneally twice a week, and 0.07 鈥，

本文編號(hào)：1921134