發(fā)布時間：2018-05-21 05:42

  本文選題：甲醛 + 甘油三酯　； 參考：《環(huán)境與健康雜志》2017年03期

【摘要】：目的觀察甲醛對肝癌細(xì)胞株HepG2細(xì)胞內(nèi)外甘油三酯(TG)含量及細(xì)胞內(nèi)極低密度脂蛋白(VLDL)分泌外排相關(guān)基因表達(dá)水平的影響。方法以0、0.004、0.02、0.1 mmol/L甲醛分別處理人肝癌HepG2細(xì)胞24、48 h,采用甘油磷酸氧化酶法(GPO-POD)測定細(xì)胞內(nèi)外TG含量,采用QRT-PCR法檢測細(xì)胞內(nèi)載脂蛋白B100(apo B100)、微粒體TG轉(zhuǎn)運(yùn)蛋白(MTTP)、載脂蛋白E(apo E)、TG水解酶(CES3)、二酰基甘油酰轉(zhuǎn)移酶1(DGAT1)、包被蛋白Ⅱ(COPⅡ)、Sar1b、低密度脂蛋白受體(LDLR)基因的表達(dá)水平。結(jié)果與陰性對照組相比,0.004、0.1 mmol/L甲醛染毒48 h后HepG2細(xì)胞內(nèi)TG含量減少,染毒24 h后上清中TG含量增加;染毒24 h后0.004、0.02、0.1 mmol/L甲醛組或染毒48 h后0.004 mmol/L甲醛組細(xì)胞內(nèi)apo B100基因表達(dá)水平升高;染毒24 h后0.004 mmol/L甲醛組或染毒48 h后0.004、0.02、0.1 mmol/L甲醛組細(xì)胞內(nèi)MTTP基因表達(dá)水平升高;染毒24 h后0.1 mmol/L甲醛組細(xì)胞內(nèi)LDLR基因表達(dá)水平降低,染毒48 h后0.02、0.1 mmol/L甲醛組細(xì)胞內(nèi)LDLR基因表達(dá)水平升高;上述差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論甲醛可能通過增加肝細(xì)胞內(nèi)apo B100和MTTP表達(dá)來促進(jìn)VLDL的分泌外排。
[Abstract]:Objective to observe the effect of formaldehyde on the content of triglyceride (TG) in HepG2 cells and the expression level of efflux related genes secreted by very low density lipoprotein (VLDL). Methods Human hepatoma HepG2 cells were treated with 0. 004 and 0. 02 mmol/L formaldehyde for 24 h, and the content of TG was measured by glycerophosphate oxidase assay (GPO-POD). QRT-PCR assay was used to detect the expression of apolipoprotein B100(apo B100, microsomal TG transporter MTTPN, apolipoprotein E(apo EtoTG hydrolase CES3, diacylglyceryl transferase 1 (DGAT1), encapsulated protein 鈪，

本文編號：1917972