本文選題：燕窩 + 肺陰虛小鼠��； 參考：《福建農(nóng)林大學(xué)》2017年碩士論文

【摘要】：燕窩是幾種金絲燕在繁殖的季節(jié)用唾液或唾液與其絨羽混合凝結(jié)成的巢窩。燕窩多產(chǎn)自印度尼西亞、泰國、我國西(南)部等東南亞國家以及北太平洋的澳大利亞等國。李時珍的《本草綱目》描述了燕窩入肺生氣、入腎滋水、入胃補脾、補而不燥的功能。燕窩因其含有豐富的營養(yǎng)物質(zhì),藥用價值極高,在全球范圍內(nèi)都深受歡迎。目前,對燕窩功能的研究很多,但燕窩對特定受損器官的調(diào)養(yǎng)與恢復(fù)影響的相關(guān)研究較少,燕窩對肺陰虛動物免疫功能影響的研究未見報道。本課題研究了燕窩對肺陰虛小鼠免疫功能的影響及機(jī)理,這將為證實燕窩具有滋養(yǎng)肺陰的功能,并應(yīng)用于未來開發(fā)燕窩調(diào)養(yǎng)與陰虛癥相關(guān)的諸多肺損傷疾病的產(chǎn)品,提供理論依據(jù),研究結(jié)果如下:1.燕窩營養(yǎng)價值高,對其氨基酸、無機(jī)元素、唾液酸、蛋白質(zhì)、脂肪、總糖和灰分含量的測定結(jié)果為:燕窩勻漿物的總氨基酸含量占49.49%(含9種必需/半必需氨基酸),蛋白質(zhì)含量占59.53%,唾液酸含量占9.47%,脂肪、總糖和灰分分別占0.04%、0.08%和2.39%,無機(jī)元素種類多樣,其中宏量元素Ca、Mg、Na和K含量豐富,且含多種人體所需的微量元素。2.利用S02熏蒸法構(gòu)建肺陰虛動物模型,研究不同濃度燕窩勻漿物對肺陰虛小鼠體征、體重、免疫器官指數(shù)、肺部病理變化及血清中細(xì)胞因子IL-2、IL-4和IFN-γ含量的影響。結(jié)果表明,燕窩中、高劑量勻漿物對提高肺陰虛小鼠的機(jī)體免疫功能有一定的作用。燕窩中、高劑量組小鼠和正常對照組一樣,沒有出現(xiàn)模型對照組小鼠嗆咳、倦伏、皮毛光澤差、凌亂脫落、煩躁易怒、食少、體重顯著下降等肺陰虛癥狀;燕窩中、高劑量組和陽性對照組小鼠的免疫器官指數(shù)與正常對照組相近,但顯著高于模型對照組(P0.05),說明燕窩中、高劑量組小鼠肺部炎癥較模型對照組輕微;小鼠肺部HE染色結(jié)果顯示,燕窩中、高劑量組小鼠肺部的炎癥情況介于正常小鼠及模型小鼠之間;燕窩中、高劑量組的IL-2、IFN-γ水平趨近于正常對照組,而顯著低于模型對照組(P0.05),各組實驗小鼠間IL-4水平無顯著差異,提示燕窩對肺陰虛小鼠的恢復(fù)可能有一定作用,且燕窩中劑量組的效果較好。3.研究燕窩中劑量對小鼠脾淋巴細(xì)胞增殖,脾淋巴細(xì)胞凋亡,肺組織中IL-8、丙二醛(MDA)水平的影響及小鼠肺組織基因的變化。燕窩中劑量組的脾淋巴細(xì)胞增殖效果顯著高于正常對照組(P0.05),而顯著低于模型對照組(P0.05),說明燕窩勻漿物能有效減緩肺陰虛小鼠的免疫應(yīng)激反應(yīng)。對于小鼠脾淋巴細(xì)胞凋亡總比例和早期凋亡比例,模型對照組均顯著高于正常對照組(P0.05),燕窩中劑量組早期凋亡細(xì)胞比例顯著低于模型對照組(P0.05),說明燕窩勻漿物對抑制脾淋巴細(xì)胞凋亡具有一定的作用。模型對照組小鼠肺組織的IL-8、MDA比其他組小鼠的水平都高,燕窩中劑量組小鼠肺組織中IL-8、MDA含量均接近正常對照組,提示燕窩勻漿物可能具有抑制機(jī)體產(chǎn)生IL-8、MDA的作用,從而抑制炎癥反應(yīng)的發(fā)生,減輕小鼠肺組織的損傷。與正常對照組相比,其他三組小鼠肺組織的共同差異基因有三個,分別為:Atrophin-1,Cytokeratin-5 和 Cyclooxygenase-2,燕窩可通過抑制Atrophin-1,Cyclooxygenase-2兩種基因表達(dá)的上調(diào)來延緩肺陰虛小鼠肺組織的損傷,從而增強機(jī)體的免疫作用。
[Abstract]:The bird's nest is a nest of saliva and saliva mixed with its feathers in the breeding season. The bird's nest is produced from Indonesia, Thailand, western (South) and other Southeast Asian countries as well as Australia and other countries in the North Pacific. Li Shizhen's < Compendium of Materia Medica > describes the bird's nest into the lungs, enters the kidney and nourish the spleen, and does not fill the spleen. Because of its rich nutrients and high medicinal value, the bird's nest is very popular in the world. At present, many studies have been made on the function of bird's nest, but few studies have been made on the effect of bird's nest on the regulation and recovery of specific damaged organs. The study of the effect of bird's nest on the immune function of lung yin asthenia animals has not been reported. The effect and mechanism of the bird's nest on the immune function of the lung yin deficiency mice will be used to confirm the function of the bird's nest to nourish the lung yin, and to provide the theoretical basis for the future development of the products related to the deficiency of the bird's nest and the deficiency of the Yin. The results are as follows: 1. the nutritive value of the bird's nest is high, and its amino acids, inorganic elements, sialic acid, and eggs are of high value. The results of the determination of white matter, fat, total sugar and ash content were as follows: the total amino acid content of the bird's nest homogenate was 49.49% (including 9 essential / semi essential amino acids), protein content was 59.53%, the content of sialic acid accounted for 9.47%, fat, total sugar and ash accounted for 0.04%, 0.08% and 2.39%, and the inorganic elements were diverse, including the macroelements Ca, Mg, Na and K. The effects of different concentrations of bird's nest homogenate on the physical signs, body weight, immune organ index, lung pathological changes and the content of cytokines IL-2, IL-4 and IFN- in the serum of lung yin asthenia mice were studied by S02 fumigation. The results showed that the high dose homogenate in the bird's nest was extracted by.2.. In the bird's nest, the high dose group, the high dose group and the normal control group, did not appear in the model control group, with no cough, tiredness, poor lustre, disorder and shedding, irritability, less food and a significant decrease in weight, and the immunity of the high dose group and the positive control group in the bird's nest. The organ index was similar to that of the normal control group, but it was significantly higher than the model control group (P0.05). It showed that the lung inflammation in the high dose group was slightly higher in the high dose group than the model control group. The lung HE staining results showed that the lung inflammation in the high dose group of mice was between the normal mice and the model mice; in the bird's nest, the high dose group was IL-2, I The level of FN- gamma is close to the normal control group, but it is significantly lower than the model control group (P0.05). There is no significant difference in the level of IL-4 between the mice in each group. It suggests that the bird's nest may have a certain effect on the recovery of the lung yin deficiency mice, and the effect of the dose group in the bird's nest is better.3. to study the proliferation of splenic lymphocyte in the mouse's nest, the apoptosis of the spleen lymphocyte and the lung. The effect of IL-8, malondialdehyde (MDA) and gene changes in lung tissue in mice. The proliferation effect of spleen lymphocyte in the middle dose group of the bird's nest was significantly higher than that of the normal control group (P0.05), but significantly lower than the model control group (P0.05), indicating that the swallow nest homogenate could effectively alleviate the immune stress response of mice with lung yin deficiency. The proportion of apoptosis and the proportion of early apoptosis in the model control group were significantly higher than that in the normal control group (P0.05). The percentage of early apoptotic cells in the middle dose group of the bird's nest was significantly lower than that of the model control group (P0.05), indicating that the swallow nest homogenate had a certain effect on the inhibition of the apoptosis of the spleen lymphocyte. The IL-8 and MDA of the lung tissue in the model control group were more than that of the other groups. The levels of IL-8 and MDA in the lung tissue of the middle dose group of the bird's nest were all close to the normal control group, suggesting that the swallow nest homogenate may inhibit the production of IL-8 and MDA in the body, thus inhibiting the occurrence of inflammation and reducing the damage of lung tissue in mice. Compared with the normal control group, the common differential genes in the lung tissues of the other three groups of mice were compared. There are three: Atrophin-1, Cytokeratin-5 and Cyclooxygenase-2. The bird's nest can postpone the injury of lung tissue in mice with lung yin deficiency by inhibiting the up regulation of the expression of Atrophin-1 and Cyclooxygenase-2, thus enhancing the immune function of the body.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R151

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