本文選題：靈芝孢子粉 + MDA　； 參考：《遵義醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:研究靈芝孢子粉對(duì)亞慢性染鎘大鼠肝臟損傷的保護(hù)作用,并探討其可能的保護(hù)途徑。方法:健康雄性SD大鼠84只,體重(90±10)g,隨機(jī)分為:正常對(duì)照組(C)、單獨(dú)染鎘組(Cadmium,Cd)和2個(gè)給藥劑量組(靈芝孢子粉0.5g/kg,1.0g/kg+Cd Cl2,即GCd L和GCdH組),每組各21只。大鼠適應(yīng)性喂養(yǎng)7d后,每天對(duì)GCdL組和GCdH組大鼠灌相應(yīng)劑量的靈芝孢子粉,C組和Cd組灌等體積蒸餾水。Cd組、GCdL組和GCd H組每隔一天腹腔注射一次氯化鎘(1.0mg/kg),每周稱重1次,并觀察大鼠的一般情況,并在第30天、60天、90天分批處死,每組各處死7只;頸動(dòng)脈取血,快速取肝臟,生理鹽水沖洗,濾紙吸干多余的水分,稱重,記錄,-80℃保存;全自動(dòng)血生化儀檢測(cè)大鼠丙氨酸氨基轉(zhuǎn)移酶(Alanine aminotransferase,ALT)和天門冬氨酸氨基轉(zhuǎn)移酶(Aspartate transaminase,AST)的濃度;光學(xué)顯微鏡觀察大鼠肝臟病理組織學(xué)變化;比色法檢測(cè)肝臟丙二醛(Malondialdehyde,MDA)含量;酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)大鼠肝臟IL-6含量;Real time RT-PCR檢測(cè)大鼠肝臟p65 m RNA、Mt-1 m RNA、Mt-2 m RNA的表達(dá)量。結(jié)果:與正常對(duì)照組比較,60d、90d的Cd組大鼠體重明顯降低(P0.05),給予靈芝孢子粉干預(yù)后,大鼠體重均有不同程度增加,在90天時(shí),GCdH組大鼠體重明顯高于Cd組(P0.05);與空白組相比,大鼠染鎘后肝臟臟器系數(shù)明顯增大(P0.05),給藥劑量組大鼠肝臟臟器系數(shù)與Cd組比較均有不同程度降低(P0.05);與空白組相比,單獨(dú)染鎘組ALT、AST明顯升高(P0.05),而靈芝孢子粉各劑量組ALT、AST均有不同程度降低,且與單獨(dú)染鎘組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);HE染色結(jié)果顯示,單獨(dú)染鎘組肝組織細(xì)胞間質(zhì)充血嚴(yán)重,細(xì)胞排列雜亂,細(xì)胞界限不清晰,而給藥組以上情況均有不同程度改善,并且高劑量組改善情況優(yōu)于中劑量組;比色法檢測(cè)MDA結(jié)果顯示,經(jīng)鎘處理大鼠肝臟MDA含量較空白組明顯增高(P0.05),給予靈芝孢子粉干預(yù)后,在60d、90d均有降低(P0.05);在30、60、90天,與空白組相比,單獨(dú)染鎘組大鼠肝臟細(xì)胞因子IL-6(白介素6)含量明顯升高(P0.05);隨著時(shí)間的延長,Cd組、GCdL組、GCdH組IL-6含量也逐漸增加(P0.05);與單獨(dú)染鎘組相比,給藥組大鼠肝臟Mt-1 m RNA、Mt-2 m RNA的表達(dá)量明顯升高(P0.05),而p65 m RNA表達(dá)明顯降低(P0.05)。結(jié)論:靈芝孢子粉對(duì)亞慢性染鎘大鼠肝臟損傷有保護(hù)作用,其機(jī)制可能與抗氧化應(yīng)激,降低炎性細(xì)胞因子IL-6表達(dá)和誘導(dǎo)MT產(chǎn)生有關(guān)。
[Abstract]:Aim: to study the protective effect of ganoderma lucidum spores powder on liver injury in rats with subchronic cadmium exposure and explore its possible protective pathway. Methods: 84 healthy male Sprague-Dawley rats, weighing 90 鹵10 g / g, were randomly divided into two groups: normal control group, single cadmium group and two dosage groups (Ganoderma lucidum spores 0.5 g / kg 路kg ~ (-1) 路kg ~ (-1) CD _ (Cl _ 2), I. e., GCd L group and GCdH group, 21 rats in each group. After 7 days of adaptive feeding, rats in GCdL group and GCdH group were given the same dose of Ganoderma lucidum spores powder group C and CD group with the same volume distilled water. CD group and GCd H group were intraperitoneally injected with 1.0 mg / kg cadmium chloride once every other day and weighed once a week. The general condition of the rats was observed, and the rats were killed in batches on the 30th day, the 60th day and the 90th day, 7 rats were killed in each group, the blood was taken from the carotid artery, the liver was taken quickly, the saline was washed, the filter paper was sucked to dry the excess water, the weight was weighed, and the records were stored at -80 鈩，

本文編號(hào)：1877853