本文選題：學(xué)習(xí)記憶 + 水迷宮��； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2015年01期

【摘要】：目的探討蘆丁對(duì)三甲基錫(Trimethyltin,TMT)損害小鼠學(xué)習(xí)記憶功能的保護(hù)作用及可能機(jī)制。方法以6~9周齡雄性BALB/c小鼠為研究對(duì)象,隨機(jī)分為生理鹽水組(對(duì)照)、TMT組、TMT+蘆丁組、蘆丁組,每組各10只;建立TMT(2.25 mg/kg·B.W.)急性暴露模型,后兩組蘆丁(10 mg/kg·B.W.)預(yù)處理1周,均腹腔注射;TMT給藥后24 h,Morris水迷宮測(cè)試各組的逃逸潛伏期,Western檢測(cè)各組海馬和皮層腦區(qū)突觸囊泡蛋白(Synaptophysin,SYP)的表達(dá)情況。結(jié)果 Morris水迷宮顯示TMT給藥后24 h,與TMT組相比,對(duì)照組和蘆丁組及TMT+蘆丁組逃逸潛伏期顯著縮短(P0.05),與蘆丁組及對(duì)照組相比,TMT+蘆丁組逃逸潛伏期無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);Western顯示TMT給藥后24 h,與對(duì)照組相比,TMT組海馬和皮層腦區(qū)SYP蛋白表達(dá)顯著降低(P0.05);與TMT組相比,TMT+蘆丁組海馬和皮層腦區(qū)SYP蛋白表達(dá)顯著升高(P0.05),與蘆丁組及對(duì)照組相比,TMT+蘆丁組海馬和皮層腦區(qū)SYP蛋白表達(dá)無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論蘆丁預(yù)處理對(duì)TMT急性暴露損害小鼠學(xué)習(xí)記憶功能有保護(hù)作用,其保護(hù)作用可能與拮抗海馬和皮層腦區(qū)SYP表達(dá)下調(diào)有關(guān)。
[Abstract]:Objective to investigate the protective effect of rutin on learning and memory impairment in mice and its possible mechanism. Methods male BALB/c mice aged 6 to 9 weeks were randomly divided into normal saline group (control group, 10 rats in each group), and TMT(2.25 mg/kg B.W. Acute exposure model, the latter two groups of rutin 10 mg/kg B.W. After preconditioning for 1 week, the escape latency and the expression of synaptic vesicle protein (Synaptophysin sinusii SYP) in hippocampus and cortical brain were detected by Morris water maze test 24 hours after intraperitoneal injection of TMT. Results Morris water maze showed that 24 hours after administration of TMT, compared with TMT group. The escape latency of control group, rutin group and TMT rutin group was significantly shortened than that of rutin group and control group. There was no significant difference in escape latency between rutin group and rutin group. Western analysis showed that the escape latency of rutin group was 24 hours after administration of TMT, and that of hippocampus and cortical brain in TMT group was higher than that in control group. Compared with TMT group, the expression of SYP protein in hippocampus and cortical brain was significantly increased in SYP group, and no significant difference was found in SYP protein expression in hippocampus and cortical brain in Rutin group compared with rutin group and control group. Conclusion Rutin preconditioning has protective effect on learning and memory function in mice with acute exposure to TMT, which may be related to the antagonism of down-regulation of SYP expression in hippocampus and cortical brain.
【作者單位】： 重慶醫(yī)科大學(xué)實(shí)驗(yàn)教學(xué)管理中心;重慶市疾病預(yù)防控制中心;第三軍醫(yī)大學(xué)勞動(dòng)衛(wèi)生學(xué)教研室;
【分類號(hào)】：R114

【共引文獻(xiàn)】

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