發(fā)布時間：2018-05-04 18:40

  本文選題：鋁暴露 + 腦皮質(zhì)　； 參考：《衛(wèi)生研究》2017年01期

【摘要】：目的研究慢性鋁暴露對腦皮質(zhì)與外周血淋巴細(xì)胞N-甲基-D-天冬氨酸受體1(NMDAR1)的影響,探討外周血淋巴細(xì)胞NMDAR1作為外周血中鋁暴露生物標(biāo)志物的可能性。方法選取2月齡健康雄性清潔級SD大鼠32只,按體重隨機分為空白組和鋁低、中、高劑量組,空白組飲用自來水,鋁低、中、高劑量組分別給予20、120、720 mg/L Al Cl3飲水,大鼠每日飲水約10 m L/100 g,連續(xù)染毒360 d。染毒結(jié)束,采用石墨爐原子吸收法測定血漿鋁和腦鋁含量,采用實時熒光定量聚合酶鏈反應(yīng)法(RT-PCR)測定腦皮質(zhì)與外周血淋巴細(xì)胞中NMDAR1基因相對表達(dá)量,酶聯(lián)免疫吸附法(ELISA)測定腦皮質(zhì)和外周血淋巴細(xì)胞中NMDAR1蛋白含量。結(jié)果對照組及鋁低、中、高劑量組血漿鋁含量分別為69.88、83.10、87.06和134.60μg/L,鋁低、中、高劑量組高于對照組,差異有統(tǒng)計學(xué)意義(P0.05);腦鋁含量分別為0.065、0.102、0.139和0.228μg/mg,鋁低、中、高劑量組高于對照組,差異有統(tǒng)計學(xué)意義(P0.05)。NMDAR1基因表達(dá)隨鋁劑量升高呈下降趨勢,腦皮質(zhì)基因相對表達(dá)量鋁中、高劑量組低于對照組(P0.05);外周血淋巴細(xì)胞NMDAR1基因表達(dá)鋁中、高劑量組低于鋁低劑量組和對照組(P0.05),鋁中、高劑量組之間比較差異無統(tǒng)計學(xué)意義(P=0.167)。腦皮質(zhì)NMDAR1蛋白含量鋁高劑量組低于對照組、鋁低劑量組(P0.05);鋁中劑量組低于對照組(P0.05);淋巴細(xì)胞NMDAR1蛋白含量鋁高劑量組低于對照組、鋁低劑量組(P0.05),與鋁中劑量組比較差異無統(tǒng)計學(xué)意義(P=0.159)。結(jié)論慢性鋁暴露可影響大鼠腦皮質(zhì)與外周血淋巴細(xì)胞NMDAR1基因相對表達(dá)量和蛋白表達(dá),隨鋁劑量的增加基因相對表達(dá)量和蛋白表達(dá)下降,可將NMDAR1作為鋁暴露外周生物標(biāo)志物進(jìn)一步研究。
[Abstract]:Objective to study the effect of chronic aluminum exposure on N- methyl -D- aspartic acid receptor 1 (NMDAR1) in the cerebral cortex and peripheral blood lymphocytes and to explore the possibility of NMDAR1 as a biomarker in the peripheral blood of the peripheral blood. Methods 32 healthy male SD rats were selected and divided into blank group and low aluminum by weight randomly. High dose group, blank group drinking tap water, aluminum low, middle, high dose group were given 20120720 mg/L Al Cl3 drinking water respectively, rats drinking water about 10 m L/100 g daily, continuous exposure to 360 D. poisoning end, graphite furnace atomic absorption method was used to determine plasma aluminum and brain aluminum content, real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used for the determination of cerebral cortex and cerebral cortex. The relative expression of NMDAR1 gene in peripheral blood lymphocytes and the content of NMDAR1 protein in cerebral cortex and peripheral blood lymphocytes were measured by enzyme linked immunosorbent assay (ELISA). Results of the control group and aluminum low, middle, high dose group, the plasma aluminum content was 69.88,83.10,87.06 and 134.60 mu g/L respectively, and the high dose group was higher than the control group, the difference was statistically significant. The content of Al (P0.05) was 0.065,0.102,0.139 and 0.228 g/mg respectively, and the high dose group of aluminum was higher than that of the control group. The difference was statistically significant (P0.05), the expression of.NMDAR1 gene decreased with the increase of aluminum dose, and the relative expression of aluminum in the cerebral cortex was lower than that of the control group (P0.05), and the NMDAR1 gene expression in peripheral blood lymphocytes was expressed. In aluminum, the high dose group was lower than the low dose al Group and the control group (P0.05), and there was no significant difference between the aluminum and the high dose groups (P=0.167). The high dose group of NMDAR1 protein in the cerebral cortex was lower than the control group, the low dose al Group (P0.05), the middle dose of aluminum group was lower than the control group (P0.05), and the high dose group of lymphocyte NMDAR1 protein content was lower than that of the high dose group. There was no significant difference between the low dose al Group (P0.05) and the aluminum dose group (P=0.159). Conclusion chronic aluminum exposure could affect the relative expression and protein expression of NMDAR1 gene in the cerebral cortex and peripheral blood lymphocytes of rats. The relative expression of gene and the expression of protein decreased with the increase of aluminum dose, and NMDAR1 could be used as the aluminum exposure week. Further study of biomarkers.

【作者單位】： 山西醫(yī)科大學(xué)公共衛(wèi)生學(xué)院勞動衛(wèi)生教研室;
【基金】：國家自然科學(xué)基金重點項目(No.81430078) 山西省教育廳研究生教育創(chuàng)新項目(No.2016SY029)
【分類號】：R114

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本文編號：1844167