本文選題：海地瓜 + 巖藻聚糖硫酸酯��； 參考：《營養(yǎng)學報》2015年04期

【摘要】：目的研究海地瓜巖藻聚糖硫酸酯(Fucoidan isolated from Acaudina molpadioides,Am-FUC)對胰島素抵抗小鼠肝糖原合成的影響。方法以高脂高糖飼料飼喂雄性C57BL/6J小鼠,建立胰島素抵抗小鼠模型。模型小鼠飼喂Am-FUC 19w后,檢測其空腹血糖、葡萄糖耐受性、胰島素水平、肝糖原含量及糖代謝關鍵酶活力。采用q RT-PCR法進一步研究小鼠肝糖原合成通路PKB/GSK-3β中關鍵基因m RNA表達。結果 Am-FUC能顯著降低模型小鼠空腹血糖水平,改善葡萄糖耐受量,提高肝糖原含量;增加肝臟中己糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)等糖原合成相關酶活力,降低糖原磷酸化酶(glycogen phosphorylase,GP)和葡萄糖-6-磷酸酶(glucose-6-phosphatase,G6Pase)等糖異生關鍵酶活力;顯著上調(diào)肝臟中IR(insulin receptor,)、IRS-2(insulin receptor substrate-2)、PI3K(phosphatidylinositol 3-hydroxy kinase,)、AKT(Protein Kinase B,)、GS(glycogen synthase)的m RNA及磷酸化蛋白的表達,抑制糖原合成負調(diào)節(jié)基因GSK-3β(glycogen synthase kinase-3β)的m RNA及磷酸化蛋白的表達。結論 Am-FUC能顯著促進模型小鼠合成肝糖原,改善胰島素抵抗,其作用機制與調(diào)控肝臟中糖代謝關鍵酶活力及PI3K/AKT/GSK-3β糖原合成通路有關。
[Abstract]:Objective to study the effect of Fucoidan isolated from Acaudina molpadioidesa Am-FUCon on hepatic glycogen synthesis in insulin-resistant mice.Methods male C57BL/6J mice were fed with high fat and high sugar diet to establish insulin resistance model.The fasting blood glucose, glucose tolerance, insulin level, liver glycogen content and the key enzyme activity of glucose metabolism were measured in model mice fed with Am-FUC for 19 weeks.The expression of m RNA, a key gene in mouse liver glycogen synthesis pathway PKB/GSK-3 尾, was further studied by Q RT-PCR method.Results Am-FUC could significantly decrease fasting blood glucose level, improve glucose tolerance, increase liver glycogen content, and increase the activities of glycogen synthase related enzymes such as hexokinase (Hexokinase) and pyruvate kinase (pyruvate kinase) in the liver.To inhibit the expression of m RNA and phosphorylated protein of GSK-3 尾 -glycogen synthase kinase-3 尾.Conclusion Am-FUC can significantly promote the synthesis of liver glycogen and improve insulin resistance in model mice. The mechanism is related to the regulation of the activity of key enzymes of glucose metabolism and the PI3K/AKT/GSK-3 尾 glycogen synthesis pathway in the liver.
【作者單位】： 中國海洋大學食品科學與工程學院;
【基金】：國家自然科學基金(No.31371876,31471684) 海洋公益性行業(yè)科研專項(No.201105029)
【分類號】：R151

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