本文選題：傳遞體 + 經(jīng)皮制劑��； 參考：《暨南大學》2012年碩士論文

【摘要】：目的: .探索制備超氧化物歧化酶傳遞體的合適工藝，分析其理化特征,最后考察其透皮吸收情況。 方法： 以薄膜-擠壓法制備超氧化物岐化酶（SOD）傳遞體，以BCA蛋白濃度檢測法測定其包封率。采用微量連苯三酚自氧化法（325nm法）來評價超氧化物歧化酶的活性。另外，考察了SOD傳遞體在大鼠皮膚的體外經(jīng)皮吸收情況。結果： 制備了粒徑均一的SOD傳遞體制劑。用BCA蛋白檢測法測定SOD濃度，在5-1000μg/ml的濃度區(qū)間內(nèi)，具有較好的線性。制備的傳遞體包封率為（57.3±0.5）%，平均粒徑是(158.4±12.5)nm，與SOD溶液以及普通脂質體相比，SOD傳遞體能透過皮膚，而前兩者未檢測到SOD。 結論： 該超氧化物歧化酶傳遞體制備方法可行，其分析方法穩(wěn)定。制備的超氧化物歧化酶傳遞體有較高的包封率，，并能透過大鼠的皮膚。
[Abstract]:Objective:
A suitable process for preparing superoxide dismutase (SOD) transfer body was explored, and its physicochemical characteristics were analyzed. Finally, the transdermal absorption of the superoxide dismutase was investigated.
Method:
The film prepared by extrusion of superoxide dismutase (SOD) transfer, the encapsulation rate was determined by BCA assay. The protein concentration of Trace Benzene even three phenolic autoxidation method (325nm method) to evaluate the superoxide dismutase activity. In addition, the effects of SOD transfer in rat skin in vitro percutaneous absorption results:
The preparation of uniform size SOD transfer agent system. The concentration of SOD protein was detected with BCA, in the concentration range of 5-1000 g/ml, with good linearity. The preparation of transfersome encapsulation was (57.3 + 0.5)%, the average particle size is (158.4 + 12.5) nm, compared with SOD solution and ordinary liposomes, SOD physical transmission through the skin, and both were not detected by SOD.
Conclusion:
The preparation method of the superoxide dismutase delivery system is feasible, and its analytical method is stable. The prepared superoxide dismutase transporter has high encapsulation efficiency and can penetrate rat skin.

【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R11

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