本文選題：竹蓀醇提物　切入點：RAW.細胞　出處：《營養(yǎng)學報》2017年04期

【摘要】：目的探討竹蓀醇提物對LPS誘導的RAW264.7細胞腫瘤壞死因子(TNF-α)、一氧化氮(NO)的分泌,TNF-α、誘導型一氧化氮合酶(iNOS)、白細胞介素6(IL-6)、白細胞介素10(IL-10)的m RNA水平以及核轉錄因子(NF-κB)p65蛋白表達的影響及可能的抗炎機制。方法以不同濃度(100、200、400μg/ml)的竹蓀醇提物作用于LPS(1μg/ml)誘導的RAW264.7小鼠巨噬細胞,采用ELISA法和Griess法檢測炎性介質TNF-α和NO的分泌量;RT-PCR法測定促炎介質TNF-α、iNOS、IL-6和抗炎介質IL-10的m RNA水平;Western blot檢測NF-κB p65蛋白的表達水平。結果與LPS組相比,200、400μg/ml的竹蓀醇提物能顯著抑制LPS誘導的RAW264.7細胞TNF-α和NO的釋放(P0.01);且不同程度抑制RAW264.7細胞中促炎介質iNOS、TNF-α和IL-6的m RNA表達水平(P0.01),劑量依賴性的促進抗炎介質IL-10的m RNA表達水平(P0.01);不同濃度的竹蓀醇提物均可顯著抑制LPS誘導RAW264.7細胞中NF-κB p65蛋白的表達,差異有統(tǒng)計學意義(P0.01)。結論竹蓀醇提物可抑制LPS誘導的RAW264.7細胞TNF-α和NO的釋放,其抗炎作用的發(fā)揮可能與抑制NF-κB p65蛋白的表達,從而調(diào)節(jié)炎性介質的基因水平有關。
[Abstract]:Objective to investigate the level of m RNA and the transcription factor NF- 魏 B)p65 protein of LPS induced tumor necrosis factor TNF- 偽 (no), inducible nitric oxide synthase (iNOS), interleukin 6 (IL 6) and interleukin 10 (IL 10) in RAW264.7 cells induced by alcohol extract of bamboo sun. Methods LPS(1 渭 g / ml (100 渭 g / ml) was used to induce macrophages in RAW264.7 mice. ELISA and Griess were used to detect the secretion of TNF- 偽 and no. RT-PCR was used to detect the levels of IL-6 and m RNA of TNF- 偽 and IL-10. The expression of NF- 魏 B p65 protein was detected by Western blot. To inhibit the release of TNF- 偽 and no in RAW264.7 cells induced by LPS, and to inhibit the expression level of m RNA of iNOS TNF- 偽 and IL-6 in RAW264.7 cells to varying degrees, and to promote the expression level of m RNA of IL-10 in a dose-dependent manner, and to increase the expression level of m RNA of IL-10 in a dose-dependent manner. The alcohol extract could significantly inhibit the expression of NF- 魏 B p65 protein in RAW264.7 cells induced by LPS. Conclusion the alcohol extract can inhibit the release of TNF- 偽 and no in RAW264.7 cells induced by LPS, and its anti-inflammatory effect may be related to the inhibition of the expression of NF- 魏 B p65 protein and the regulation of the gene level of inflammatory mediators.
【作者單位】： 遼寧師范大學生命科學學院遼寧省生物技術與分子藥物研發(fā)重點實驗室;
【基金】：國家級大學生創(chuàng)新創(chuàng)業(yè)訓練計劃(No.201610165042) 遼寧省教育廳科學研究一般項目(No.L201683675)
【分類號】：R151

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