本文選題：微波輻射　切入點(diǎn)：能量代謝　出處：《中國(guó)病理生理雜志》2015年04期

【摘要】：目的:探討不同強(qiáng)度微波輻射對(duì)大鼠心臟的損傷作用,并初步探討其作用機(jī)制。方法:分別以強(qiáng)度為500、1 000、1 500和2 000 W/m2,頻率為2 450 MHz微波照射大鼠6 min,照射后6 h取大鼠心臟,檢測(cè)心肌細(xì)胞中ATP含量、線粒體呼吸鏈復(fù)合體Ⅳ和Ⅴ活性,透射電鏡觀察心臟組織超微結(jié)構(gòu)的改變,Muse細(xì)胞分析儀檢測(cè)心肌細(xì)胞凋亡,Western blotting檢測(cè)心肌細(xì)胞內(nèi)cleaved caspase-3蛋白的表達(dá)。結(jié)果:隨著微波輻射強(qiáng)度的增加,大鼠心肌細(xì)胞中ATP含量、線粒體呼吸鏈復(fù)合體Ⅳ和Ⅴ活性呈現(xiàn)降低趨勢(shì),與對(duì)照組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。透射電鏡結(jié)果顯示,微波輻射致細(xì)胞線粒體數(shù)量減少且形態(tài)異常,大部分線粒體溶解空化,基質(zhì)明顯腫脹。心肌細(xì)胞凋亡檢測(cè)結(jié)果顯示,微波輻射能夠誘導(dǎo)大鼠心肌細(xì)胞發(fā)生凋亡,且隨著微波輻射強(qiáng)度的增加,細(xì)胞凋亡率呈上升趨勢(shì)(P0.05)。Western blotting結(jié)果顯示,微波輻射后心肌細(xì)胞內(nèi)cleaved caspase-3蛋白表達(dá)水平隨著輻射強(qiáng)度的增加呈上升趨勢(shì)(P0.05)。結(jié)論:微波輻射對(duì)大鼠心臟具有明顯的損傷作用,能夠引起心臟能量代謝出現(xiàn)異常,誘發(fā)心肌細(xì)胞發(fā)生凋亡。
[Abstract]:Objective: to investigate the effects of microwave irradiation of different intensities on rat heart injury and its mechanism. Methods: the rat hearts were irradiated with microwave at a frequency of 2 450 MHz for 6 mins and 2 000 W / m 2 at the intensities of 500 min and 2 000 W / m 2, respectively, and the hearts of the rats were harvested 6 hours after irradiation. The contents of ATP and the activities of mitochondrial respiratory chain complexes 鈪，

本文編號(hào)：1682997