本文選題：矽肺　切入點(diǎn)：多聚鳥嘌呤核苷酸　出處：《中國職業(yè)醫(yī)學(xué)》2017年01期

【摘要】：目的觀察多聚鳥嘌呤核苷酸(Poly G)對染矽塵大鼠肺纖維化的干預(yù)作用,并探討其作用機(jī)制。方法無特定病原體級健康成年雄性SD大鼠隨機(jī)分為對照組、矽肺模型組和4個(gè)干預(yù)組(即干預(yù)1、2、3、4次組),每組5只。除對照組外,其余5組大鼠均以非暴露式氣管插管法一次性予質(zhì)量濃度為50.0 g/L的矽塵混懸液1.0 m L造模,4個(gè)干預(yù)組大鼠分別于造模后第1~21天內(nèi)腹腔注射1、2、3、4次劑量為2.5 mg/kg體質(zhì)量的Poly G。造模后28 d處死大鼠,觀察肺部組織病理學(xué)改變情況,以Ashcroft評分法評估肺纖維化程度,以蛋白免疫印跡法測定肺組織內(nèi)膠原樣結(jié)構(gòu)巨噬細(xì)胞受體(MARCO)、轉(zhuǎn)化生長因子-β1(TGF-β1)、E-鈣黏蛋白(E-cad)、波形蛋白(Vimentin)和α-平滑肌肌動(dòng)蛋白(α-SMA)的蛋白相對表達(dá)水平。結(jié)果矽肺模型組大鼠肺泡腔內(nèi)有大量塵細(xì)胞聚集,肺間質(zhì)內(nèi)出現(xiàn)彌漫性的膠原沉積,說明矽肺造模成功;干預(yù)1次組大鼠肺泡結(jié)構(gòu)基本完整,肺纖維化程度較矽肺模型組明顯減輕。與對照組比較,矽肺模型組大鼠肺組織Ashcroft評分和MARCO、TGF-β1、Vimentin、α-SMA的蛋白相對表達(dá)水平均升高(P0.05),E-cad相對表達(dá)水平下降(P0.05);與矽肺模型組比較,干預(yù)1次組大鼠肺組織的TGF-β1、Vimentin和α-SMA的蛋白相對表達(dá)水平均下降(P0.05),E-cad相對表達(dá)水平升高(P0.05)。Poly G干預(yù)次數(shù)與大鼠肺組織的Ashcroft評分和MARCO、TGF-β1、E-cad、Vimentin、α-SMA的蛋白相對表達(dá)水平之間均無劑量-效應(yīng)關(guān)系。結(jié)論 Poly G可改善矽塵所致的大鼠肺組織炎癥和纖維化,以在造模后第1天進(jìn)行一次性干預(yù)(2.5 mg/kg體質(zhì)量,腹腔注射)效果較好,其機(jī)制可能與低劑量Poly G抑制上皮間質(zhì)轉(zhuǎn)化的進(jìn)程從而減少膠原合成有關(guān)。
[Abstract]:Objective to observe the effect of Poly G on pulmonary fibrosis in silicotic rats. Methods healthy male SD rats with no specific pathogens were randomly divided into control group. Silicosis model group and 4 intervention groups (5 rats in each group, 5 rats in each group, except the control group), The rest of the five groups were treated with unexposed endotracheal intubation for a single dose of 50.0 g / L silica dust suspension 1.0 mL. The rats in the four intervention groups were injected intraperitoneally with 1 2 渭 g / L 1. 5 mg/kg body mass respectively within 21 days after the establishment of the model, and the other 5 groups were given intraperitoneal injection of 2. 5 mg / L silica dust suspension at a dose of 2. 5 g / L respectively within 21 days after the establishment of the model. The rats were killed 28 days after Poly G. The changes of lung histopathology were observed and the degree of pulmonary fibrosis was evaluated by Ashcroft score. The relative expression levels of collagen like macrophage receptor (MARCOG), transforming growth factor- 尾 1 (TGF- 尾 1) E-cadn (vimentin) and 偽 -SMAs in lung tissue were measured by Western blot. Results the results showed that the expression of 偽 -SMAs in silicosis model group was higher than that in control group. In the alveolar cavity of rats, a large number of dust cells gathered, The diffuse collagen deposition in the interstitial tissue of the lung indicated that the silicosis model was successful, the alveolar structure of the rats in the first intervention group was basically intact, the degree of pulmonary fibrosis was significantly reduced than that in the silicosis model group, and compared with the control group, In silicosis model group, the Ashcroft score of lung tissue and the relative expression level of MARCO-TGF- 尾 1 Vimentin, 偽 -SMA protein were increased. The relative expression levels of TGF- 尾 1 Vimentin and 偽 -SMA in the lung tissue of the first intervention group were decreased. The relative expression level of P0.05TGF- 尾 1 E-cadVimentin, 偽 -SMA protein was not dose-effective between the relative expression level of P0.05TGF- 尾 1 E-cadVimentin, 偽 -SMA protein and the Ashcroft score of lung tissue and the relative expression level of 偽 -SMA protein. Conclusion Poly G can improve pulmonary inflammation and fibrosis induced by silica dust in rats. The effect of one-off intervention on the first day after the model was 2. 5 mg/kg (intraperitoneal injection) might be related to the inhibition of the process of epithelial interstitial transformation by low dose Poly G and the reduction of collagen synthesis.
【作者單位】： 華北理工大學(xué)公共衛(wèi)生學(xué)院;新鄉(xiāng)醫(yī)學(xué)院公共衛(wèi)生學(xué)院;
【基金】：國家自然科學(xué)基金(81273017) 河南省科技攻關(guān)項(xiàng)目(162102310491)
【分類號】：R135.2;R563

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營養(yǎng)學(xué)報(bào);1957年04期

2 陳偉強(qiáng);趙善廣;;自制注射用大鼠固定裝置[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報(bào);2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2001年03期

6 戴英,陸群;復(fù)方H_(505)對Wistar大鼠外周血的血液流變學(xué)指標(biāo)的影響[J];中國血液流變學(xué)雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長斌;+Gz暴露時(shí)間對大鼠記憶功能和行為的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2003年01期

8 呂學(xué)軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國職業(yè)醫(yī)學(xué);2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學(xué)院學(xué)報(bào);2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡易的大鼠保定方法[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2004年01期

相關(guān)會議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：1654413