本文選題：輔助改善記憶　切入點：保健食品　出處：《湖南師范大學》2012年碩士論文　論文類型：學位論文

【摘要】：隨著社會競爭壓力的不斷增大和老年化社會的擴大,輔助改善記憶力類保健品正步入人們的學習工作和日常生活中,相關(guān)治療和預防老年人神經(jīng)退行性疾病的保健食品種類數(shù)目也日益增多。但是由于保健食品中違禁添加藥物的現(xiàn)象屢禁不止,而檢測方法相對被動滯后,其添加的化學藥物的副作用嚴重影響了人們(尤其是肝、腎功能減弱的中老年人)的身體健康。因此,建立有效、快速的監(jiān)控手段對提前預防和實時檢測違禁添加物的工作具有重要的現(xiàn)實意義。本論文概述了改善記憶力類保健食品的概念及作用、促智藥的分類、作用機理和代表藥物,通過比較現(xiàn)有常用的違禁藥物檢測方法,得出高效液相色譜與質(zhì)譜技術(shù)在篩選和檢測未知的違禁藥物領(lǐng)域所具有的特點及優(yōu)勢。然后利用HPLC-DAD聯(lián)用、HPLC-MS譜聯(lián)用的方法測定改善記憶力類保健食品中潛在添加多種違禁藥物,比較了不同質(zhì)譜條件下目標物的響應和特征碎片離子情況,并初步建立了促進記憶力類的化學合成藥物相關(guān)質(zhì)譜庫,以期達到有效篩選和監(jiān)控非法添加藥物的目的。 本論文主要研究工作如下： 1.分析常見的6種益智類藥物在不同的質(zhì)譜條件下的響應情況。6種等摩爾濃度的目標物用不同的流動相(甲醇：水=1：1和乙腈：水=1：1)、流動注射到不同的離子源(ESI源、APCI源及DCBI源),得到相應的質(zhì)譜圖和特征碎片離子,通過比較每個物質(zhì)的質(zhì)譜行為的差異性來分析其響應差異的主要原因。 2. HPLC-DAD聯(lián)用檢測輔助改善記憶力類保健食品中12種潛在的促智類藥物。優(yōu)化12種目標物的液相色譜分離條件,根據(jù)其目標物的保留值和DAD光譜圖能快速、準確的同時測定復雜樣品中的疑似違禁添加物,該方法樣品處理簡單,具有檢測限低、可信度高、靈敏度高的特點,應用范圍廣,可為質(zhì)量監(jiān)督部門提供有力依據(jù)。 3.建立了HPLC-MS聯(lián)用檢測保健食品中11種鈣拮抗劑類藥物。通過優(yōu)化11種鈣拮抗劑類藥物的液相色譜分離條件和質(zhì)譜的條件,使目標物具有更好的分離效果和質(zhì)譜響應值,運用質(zhì)譜進行定性和定量分析,其結(jié)果準確度高、檢測限低、靈敏度好,通過解析其質(zhì)譜裂解規(guī)律可為未知樣品中添加該類藥物或結(jié)構(gòu)衍生物提供參照。 4.建立輔助改善記憶力類藥物多級質(zhì)譜數(shù)據(jù)庫。為建立一種有效的篩選違禁藥物添加的方法,本實驗室有必要建立一套準確性高,信息豐富的質(zhì)譜數(shù)據(jù)庫,未知樣品可根據(jù)這些特征譜圖(包括一級、二級及多級質(zhì)譜圖)在缺乏對照品或非法添加了未知化學藥的情況下,將所采集的信息搜索相匹配的化學藥物,由此可實現(xiàn)短時間內(nèi)對未知成分進行初步篩選和確認的目的。
[Abstract]:With the increasing competitive pressure in society and the expansion of aging society, health products such as auxiliary memory improvement are stepping into people's study work and daily life. The number of health foods related to the treatment and prevention of neurodegenerative diseases in the elderly is also increasing. The side effects of the addition of chemical drugs seriously affect the health of people, especially the middle and old people with weakened liver and kidney functions. Rapid monitoring is of great practical significance for the prevention and real-time detection of prohibited additives in advance. This paper summarizes the concept and role of memory health food, the classification, action mechanism and representative drugs of antipsychotic drugs. By comparing the commonly used methods for detecting illicit drugs, The characteristics and advantages of high performance liquid chromatography (HPLC) and mass spectrometry in the field of screening and detection of unknown prohibited drugs were obtained. Then, the potential addition of various prohibited drugs in memory improving health foods was determined by HPLC-DAD combined with HPLC-MS. The response and characteristic fragment ions of the target were compared under different mass spectra, and a memory promoting chemosynthetic drug related mass spectrometry library was established in order to screen and monitor the illicit drug addition effectively. The main work of this thesis is as follows:. 1. Analysis of the response of 6 kinds of common probiotics under different mass spectrometry conditions. 6 different mobile phases with equal molar concentrations (methanol: water 1: 1 and acetonitrile: water 1: 1), flow injection into different ion source / ESI source (methanol: water 1: 1) and acetonitrile (acetonitrile: water 1: 1), flow injection into different ion source ESI source. The corresponding mass spectra and characteristic fragment ions were obtained from APCI source and DCBI source. The main reasons for the difference in response were analyzed by comparing the mass spectrum behavior of each substance. 2. Twelve potential intelligence promoting drugs in health food for memory improvement were detected by HPLC-DAD. The liquid chromatographic separation conditions of the 12 target compounds were optimized, and the retention values of the target compounds and the DAD spectrogram were rapidly obtained. The method has the advantages of simple sample treatment, low detection limit, high reliability, high sensitivity and wide application range, which can provide strong basis for quality supervision department. 3. HPLC-MS was used to detect 11 calcium antagonists in health food. By optimizing the separation conditions of 11 calcium antagonists by liquid chromatography and mass spectrometry, the target had better separation effect and mass spectrum response value. The results of qualitative and quantitative analysis by mass spectrometry are of high accuracy, low detection limit and good sensitivity. It can be used as a reference for adding this kind of drugs or structural derivatives to unknown samples by analyzing the cracking law of mass spectrometry. 4. To establish a multistage mass spectrometry database for auxiliary memory improvement drugs. In order to establish an effective method for screening illicit drug additions, it is necessary for our laboratory to establish a highly accurate and informative mass spectrometry database. Unknown samples may search for the collected information on the basis of these characteristic spectra (including primary, secondary and multistage mass spectrometry) in the absence of a reference substance or in the case of the illegal addition of unknown chemicals, to match the collected information, Therefore, the objective of screening and confirming unknown components in a short time can be achieved.
【學位授予單位】：湖南師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R155.5;R917

【參考文獻】

相關(guān)期刊論文 前10條

1 張志濤;;5種吡咯烷酮衍生物的電噴霧離子阱質(zhì)譜分析初探[J];安徽醫(yī)藥;2009年05期

2 劉飛;;色譜-質(zhì)譜聯(lián)用技術(shù)在食品分析中的應用[J];質(zhì)量技術(shù)監(jiān)督研究;2009年06期

3 陳煥文;胡斌;張燮;;復雜樣品質(zhì)譜分析技術(shù)的原理與應用[J];分析化學;2010年08期

4 張娟紅;王榮;謝華;賈正平;張強;;快速建立LC-MS/MS分析生物樣品的方法[J];分析儀器;2010年03期

5 李曉雯;沈保華;卓先義;;液相色譜-高分辨質(zhì)譜聯(lián)用技術(shù)在毒物篩選分析中的應用[J];法醫(yī)學雜志;2011年05期

6 黃國燕,張永慧,黃清梅;保健食品改善記憶作用人體試食試驗效果觀察[J];廣東衛(wèi)生防疫;2000年02期

7 許晉星;;HPLC法測定茴拉西坦分散片的有關(guān)物質(zhì)[J];廣東藥學院學報;2009年04期

8 管孝鞠;促智藥研究進展[J];國外醫(yī)藥.合成藥.生化藥.制劑分冊;1996年04期

9 陳子晟;治療阿爾茨海默病的非膽堿能藥物研究[J];國外醫(yī)學.藥學分冊;2001年04期

10 白滿英;李翌晨;;談健腦益智食品的功能成分及其保健機理[J];東方食療與保健;2007年09期

，

本文編號：1652116