本文選題：N　切入點(diǎn)：N-二烷基脲　出處：《北京服裝學(xué)院》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：本文采用噻唑藍(lán)(MTT)比色法評(píng)價(jià)了4種烷基酚聚氧乙烯醚系列表面活性劑(APEO)對(duì)人宮頸癌細(xì)胞(HeLa)及中華倉(cāng)鼠卵巢細(xì)胞(CHO)的細(xì)胞毒性;采用MTT比色法評(píng)價(jià)了3種N,N-二烷基脲系列化合物對(duì)人胚皮膚成纖維細(xì)胞(CCC-ESF-1)的增殖抑制作用;采用3H-TdR滲入法評(píng)價(jià)了3種脂肪醇聚氧乙烯醚類表面活性劑(AEO)對(duì)人胚皮膚成纖維細(xì)胞的增殖抑制作用。較為系統(tǒng)地研究了三類物質(zhì)的細(xì)胞毒性。實(shí)驗(yàn)結(jié)果表明:三類物質(zhì)對(duì)細(xì)胞的增殖抑制率均隨其濃度的增大而增大,但毒性差異明顯。烷基酚聚氧乙烯醚OP-7、OP-9、NP-10和NP-25的濃度為0.1mg/ml時(shí),其對(duì)HeLa細(xì)胞的增殖抑制率分別為90.23%、87.76%、89.56%和24.86%;對(duì)CHO細(xì)胞的增殖抑率分別為89.34%、85.95%、90.97%和21.31%。AEO-5、AEO-9和AEO-15濃度為0.1mg/ml時(shí),對(duì)皮膚細(xì)胞有較強(qiáng)的增殖抑制作用,抑制率分別為99.53%、98.60%和63.15%。而乙基脲、丙基脲和丁基脲濃度為0.1mg/ml時(shí),對(duì)皮膚細(xì)胞的增殖抑制作用不明顯,當(dāng)濃度為5mg/ml時(shí),對(duì)皮膚細(xì)胞具有了較強(qiáng)的增殖抑制作用,抑制率分別為61.94%、62.50%和86.94%。因此可知,這三類物質(zhì)達(dá)到一定濃度后,均對(duì)細(xì)胞的生長(zhǎng)有明顯的抑制作用,并呈現(xiàn)濃度依賴性,但N,N-二烷基脲對(duì)細(xì)胞的毒性遠(yuǎn)小于烷基酚聚氧乙烯醚和脂肪醇聚氧乙烯醚;脂肪醇聚氧乙烯醚和烷基酚聚氧乙烯醚類化合物EO數(shù)增大,對(duì)細(xì)胞的毒性逐漸減小;三種N,N-二烷基脲類物質(zhì)隨碳鏈長(zhǎng)度的增大,對(duì)細(xì)胞的毒性也逐漸增大。
[Abstract]:The cytotoxicity of four kinds of alkylphenol polyoxyethylene ether (APEO) on human cervical cancer cell line (HeLa) and Chinese hamster ovarian cell (Cho) was evaluated by MTT colorimetry. MTT colorimetric assay was used to evaluate the inhibitory effects of three N- N- dialkylurea series on the proliferation of human embryonic skin fibroblasts (CCC-ESF-1). The inhibitory effects of three fatty alcohol polyoxyethylene ether surfactants (AEO) on the proliferation of human embryonic skin fibroblasts were evaluated by 3H-TdR method. The cytotoxicity of three kinds of substances was systematically studied. The inhibition rate of cell proliferation increased with the increase of its concentration. When the concentration of OP-7 and NP-25 was 0.1 mg / ml, the inhibition rates of the proliferation of HeLa cells were 90.23% and 89.56%, respectively, and the inhibition rates of CHO cells were 89.34% and 21.31%, respectively. AEO-5AEO-9 and AEO-15 concentration were 0.1 mg / ml, respectively, in the presence of alkylphenol polyoxyethylene ether (OP-7OP-9NP-10) and NP-25 (0.1 mg / ml), the inhibitory rates on the proliferation of CHO cells were 87.76% and 24.86%, respectively, when the concentrations of OP-7 and OP-9NP-10 and NP-25 were 0.1 mg / ml, respectively, the inhibitory rates on the proliferation of CHO cells were 89.34 45%, 21.31% and 21.31%, respectively. The inhibition rate was 98.60% and 63.15%, respectively. When the concentration of ethyl urea, propyl urea and Ding Ji urea was 0.1 mg / ml, the inhibitory effect on the proliferation of skin cells was not obvious, and when the concentration was 5 mg / ml, the inhibitory effect on the proliferation of skin cells was not obvious. The inhibition rates were 61.94%, 62.50% and 86.94%, respectively. Therefore, when these three kinds of substances reached a certain concentration, they all had obvious inhibitory effects on the growth of the cells, and showed a concentration-dependent manner. However, the toxicity of N-dialkylurea to cells was much less than that of alkylphenol polyoxyethylene ether and fatty alcohol polyoxyethylene ether, and the EO number of fatty alcohol polyoxyethylene ether and alkylphenol polyoxyethylene ether compounds increased, and the toxicity of fatty alcohol polyoxyethylene ether and alkyl phenol polyoxyethylene ether compounds decreased gradually. The cytotoxicity of three N- N-dialkyl ureas increased with the length of carbon chain.
【學(xué)位授予單位】：北京服裝學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 李燕云,尹振晏,朱嚴(yán)瑾;抗靜電劑綜述[J];北京石油化工學(xué)院學(xué)報(bào);2003年01期

2 郝繼偉;淺談香椿的資源價(jià)值與利用[J];江蘇農(nóng)業(yè)科學(xué);2003年05期

3 許樹文;劉華;;紡織品服裝與皮膚微生態(tài)[J];浙江紡織服裝職業(yè)技術(shù)學(xué)院學(xué)報(bào);2005年04期

4 王學(xué)川,邱白玉;表面活性劑的毒性問題[J];日用化學(xué)品科學(xué);2005年06期

5 張昌輝;謝瑜;;烷基酚聚氧乙烯醚的安全性問題[J];日用化學(xué)品科學(xué);2007年02期

6 陳榮圻;;烷基酚聚氧乙烯醚(APEO)的禁用和代用[J];印染;2006年12期

7 安飛云;廖春華;邊寰峰;;改良MTT法檢測(cè)HePG-2細(xì)胞增殖的研究[J];毒理學(xué)雜志;2006年03期

，

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