本文選題：海兔素　切入點(diǎn)：酒精性肝損傷　出處：《衛(wèi)生研究》2015年02期 　論文類型：期刊論文

【摘要】：目的通過觀察酒精性肝損傷大鼠NF-кB p65、i NOS和COX-2的表達(dá),探討海兔素對(duì)酒精性肝損傷的保護(hù)機(jī)制。方法 (1)8周齡雄性Wistar大鼠,按體重隨機(jī)分為6組(空白對(duì)照組,酒精模型組,陽(yáng)性對(duì)照組,海兔素低、中、高劑量組),每組10只。各組均采用灌胃方式給藥,每日一次,連續(xù)6周。除空白對(duì)照組每日灌胃生理鹽水外,其余各組前2周每日給予50%乙醇8 ml/kg,后4周提高至12 ml/kg。海兔素低、中、高劑量組每日還同時(shí)分別給予海兔素50、100和150 mg/kg BW,陽(yáng)性對(duì)照組則每日給予200 mg/kg甘草酸二銨。實(shí)驗(yàn)結(jié)束后ELISA法檢測(cè)大鼠血漿中i NOS和COX-2活性。(2)8周齡成年雄性Wistar大鼠45只,按體重隨機(jī)分為3組,空白對(duì)照組每日生理鹽水灌胃,酒精模型組和海兔素組每日給予50%乙醇8 ml/kg灌胃2周,然后將劑量提高至12 ml/kg,持續(xù)6周。海兔素組每日酒精灌胃前4 h,先給予150 mg/kg海兔素灌胃。8周后經(jīng)二步膠原酶技術(shù)分離培養(yǎng)獲取大鼠原代肝細(xì)胞,每組大鼠各獲得一組原代肝細(xì)胞。Western Blot法檢測(cè)大鼠原代肝細(xì)胞NF-кB p65蛋白的表達(dá)。結(jié)果酒精模型組i NOS與COX-2活性均明顯高于空白對(duì)照組(P0.05);與酒精模型組相比,海兔素低、中、高劑量組i NOS、COX-2活性均有所降低(P0.05)。并且海兔素高劑量組i NOS及COX-2的活性與陽(yáng)性對(duì)照組相比差異無統(tǒng)計(jì)學(xué)意義。酒精模型組NF-кB p65蛋白的表達(dá)明顯高于空白對(duì)照組(P0.05),是空白對(duì)照組的1.5倍;而海兔素干預(yù)組NF-кB p65蛋白表達(dá)明顯被抑制(P0.05),與酒精模型組相比,下降52.7%。結(jié)論海兔素可通過抑制NF-кB、i NOS及COX-2的表達(dá),對(duì)酒精性肝損傷起到一定的保護(hù)作用。
[Abstract]:Objective to observe the expression of NOS and COX-2 in rats with alcoholic liver injury and to explore the protective mechanism of haitulin on alcoholic liver injury. Methods male Wistar rats of 8 weeks old were randomly divided into 6 groups according to their body weight (blank control group, alcohol model group). Positive control group, low, medium and high dose groups, 10 rats in each group. Each group was administered by gavage once a day for 6 weeks, except for the blank control group. The other groups were given 50% ethanol 8 ml / kg per day for the first 2 weeks and increased to 12 ml / kg for 4 weeks. The high dose group was also given 50,100 and 150 mg/kg BW daily, while the positive control group was given 200 mg/kg diammonium glycyrrhizinate daily. After the experiment, the plasma I NOS and COX-2 activities were detected by ELISA method in 45 adult male Wistar rats at the age of 8 weeks. The rats were randomly divided into 3 groups according to their body weight. The blank control group was fed with normal saline daily, the alcohol model group and the sea rabbit group were given 50% ethanol 8 ml/kg daily for 2 weeks. Then the dose was increased to 12 ml / kg for 6 weeks. The primary rat hepatocytes were isolated and cultured by two-step collagenase technique 4 hours before alcohol was given to the rats in the sea rabbit injection group for 4 hours before oral administration for 1. 8 weeks, and then cultured by two steps collagenase technique, and the primary rat hepatocytes were isolated and cultured by two steps collagenase technique. One group of primary hepatocytes was obtained from each group. Western Blot assay was used to detect the expression of NF- NOS B p65 protein in primary rat hepatocytes. Results the activities of I NOS and COX-2 in the alcohol model group were significantly higher than those in the control group (P 0.05). The activity of COX-2 was decreased in high dose group, and the activity of I NOS and COX-2 in high dose group was not significantly different from that in positive control group. The expression of NF- FB p65 protein in alcohol model group was significantly higher than that in control group (P 0. 05), and the expression of I NOS and COX-2 in high dose group was significantly higher than that in control group (P 0. 05). 1.5 times of the blank control group; However, the expression of NF-1 B p65 protein was significantly inhibited in the sea rabbit intervention group, which was 52.7% lower than that in the alcohol model group. Conclusion it can protect the alcoholic liver injury by inhibiting the expression of NOS and COX-2.
【作者單位】： 青島大學(xué)醫(yī)學(xué)院營(yíng)養(yǎng)研究所;青島大學(xué)醫(yī)學(xué)院細(xì)胞與分子生物學(xué)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(No.31171671) “十二五”農(nóng)村領(lǐng)域國(guó)家科技計(jì)劃課題(No.2012BAD33B01-2)
【分類號(hào)】：R151

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相關(guān)期刊論文 前6條

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